Spectrophotometric and electrochemical study of protolytic equilibria of some oximes-acetylcholinesterase reactivators
Abstract
Newly synthesized oximes, mono and bis imidazole derivatives, which promise to be more effective acetylcholinesterase reactivators than standard antidotes used, were investigated by spectrophotometric and electrochemical methods. The electrochemical investigations confirmed the existence of overlapping equilibria, obtained by spectrophotometric methods. Dissociation constants of those oximes were also obtained by numerical treatment of overlapping equilibria, using the Lavendberg-Marquardt least square method, and when compared with the same for some similar compounds, were found to be very effective acetylcholinesterase reactivators. The distribution of ionic forms of the investigated oximes, as a dependence of pH values, was calculated from the obtained values of dissociation constants. The results indicated that many oxime anions will be available at physiological pH 7.4 and a relative increased ability to reactivate inhibited acetylcholinesterase could be expected.
Keywords:
Cyclic voltammetry / Oximes / Protolytic equilibria / Spectrophotometry measurementsSource:
Journal of Pharmaceutical and Biomedical Analysis, 1999, 20, 4, 655-662Publisher:
- Pergamon-Elsevier Science Ltd, Oxford
DOI: 10.1016/S0731-7085(99)00056-4
ISSN: 0731-7085
PubMed: 10704134
WoS: 000080938200004
Scopus: 2-s2.0-0033055524
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Institution/Community
PharmacyTY - JOUR AU - Čakar, Mira AU - Vasić, Vesna AU - Petkovska, LT AU - Stojić, DL AU - Avramov-Ivić, M AU - Milovanović, GA PY - 1999 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/218 AB - Newly synthesized oximes, mono and bis imidazole derivatives, which promise to be more effective acetylcholinesterase reactivators than standard antidotes used, were investigated by spectrophotometric and electrochemical methods. The electrochemical investigations confirmed the existence of overlapping equilibria, obtained by spectrophotometric methods. Dissociation constants of those oximes were also obtained by numerical treatment of overlapping equilibria, using the Lavendberg-Marquardt least square method, and when compared with the same for some similar compounds, were found to be very effective acetylcholinesterase reactivators. The distribution of ionic forms of the investigated oximes, as a dependence of pH values, was calculated from the obtained values of dissociation constants. The results indicated that many oxime anions will be available at physiological pH 7.4 and a relative increased ability to reactivate inhibited acetylcholinesterase could be expected. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Journal of Pharmaceutical and Biomedical Analysis T1 - Spectrophotometric and electrochemical study of protolytic equilibria of some oximes-acetylcholinesterase reactivators VL - 20 IS - 4 SP - 655 EP - 662 DO - 10.1016/S0731-7085(99)00056-4 ER -
@article{ author = "Čakar, Mira and Vasić, Vesna and Petkovska, LT and Stojić, DL and Avramov-Ivić, M and Milovanović, GA", year = "1999", abstract = "Newly synthesized oximes, mono and bis imidazole derivatives, which promise to be more effective acetylcholinesterase reactivators than standard antidotes used, were investigated by spectrophotometric and electrochemical methods. The electrochemical investigations confirmed the existence of overlapping equilibria, obtained by spectrophotometric methods. Dissociation constants of those oximes were also obtained by numerical treatment of overlapping equilibria, using the Lavendberg-Marquardt least square method, and when compared with the same for some similar compounds, were found to be very effective acetylcholinesterase reactivators. The distribution of ionic forms of the investigated oximes, as a dependence of pH values, was calculated from the obtained values of dissociation constants. The results indicated that many oxime anions will be available at physiological pH 7.4 and a relative increased ability to reactivate inhibited acetylcholinesterase could be expected.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Journal of Pharmaceutical and Biomedical Analysis", title = "Spectrophotometric and electrochemical study of protolytic equilibria of some oximes-acetylcholinesterase reactivators", volume = "20", number = "4", pages = "655-662", doi = "10.1016/S0731-7085(99)00056-4" }
Čakar, M., Vasić, V., Petkovska, L., Stojić, D., Avramov-Ivić, M.,& Milovanović, G.. (1999). Spectrophotometric and electrochemical study of protolytic equilibria of some oximes-acetylcholinesterase reactivators. in Journal of Pharmaceutical and Biomedical Analysis Pergamon-Elsevier Science Ltd, Oxford., 20(4), 655-662. https://doi.org/10.1016/S0731-7085(99)00056-4
Čakar M, Vasić V, Petkovska L, Stojić D, Avramov-Ivić M, Milovanović G. Spectrophotometric and electrochemical study of protolytic equilibria of some oximes-acetylcholinesterase reactivators. in Journal of Pharmaceutical and Biomedical Analysis. 1999;20(4):655-662. doi:10.1016/S0731-7085(99)00056-4 .
Čakar, Mira, Vasić, Vesna, Petkovska, LT, Stojić, DL, Avramov-Ivić, M, Milovanović, GA, "Spectrophotometric and electrochemical study of protolytic equilibria of some oximes-acetylcholinesterase reactivators" in Journal of Pharmaceutical and Biomedical Analysis, 20, no. 4 (1999):655-662, https://doi.org/10.1016/S0731-7085(99)00056-4 . .