Retention mechanism assessment and method development for the analysis of iohexol and its related compounds in hydrophilic interaction liquid chromatography
Само за регистроване кориснике
2014
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Hydrophilic interaction liquid chromatography (HILIC) has emerged in recent years as a valuable alternative to reversed-phase liquid chromatography in the analysis of polar compounds. Research in HILIC is divided into two directions: the assessment of the retention mechanism and retention behavior, and the development of HILIC methods. In this work, four polar neutral analytes (iohexol and its related compounds A, B, and C) were analyzed on two silica and two diol columns in HILIC mode with the aim to investigate thoroughly the retention mechanisms and retention behavior of polar neutral compounds on these four columns. The adsorption and partition contribution to the overall HILIC retention mechanism was investigated by fitting the retention data to linear (adsorption and partition) and nonlinear (mixed-retention and quadratic) theoretical models. On the other hand, the establishment of empirical second-order polynomial retention models on the basis of D-optimal design made possible t...he estimation of the simultaneous influence of several mobile-phase-related factors. Furthermore, these models were also used as the basis for the application of indirect modeling of the selectivity factor and a grid point search approach in order to achieve the optimal separation of analytes. After the optimization goals had been set, the grids were searched and the optimal conditions were identified. Finally, the optimized method was subjected to validation.
Кључне речи:
Hydrophilic interaction liquid chromatography / Iohexol / Retention models / Retention mechanisms / D-optimal design / Method optimizationИзвор:
Analytical and Bioanalytical Chemistry, 2014, 406, 17, 4217-4232Издавач:
- Springer Heidelberg, Heidelberg
Финансирање / пројекти:
- Моделовање различитих хроматографских система са хемометријским приступом у фармацеутској анализи (RS-MESTD-Basic Research (BR or ON)-172052)
DOI: 10.1007/s00216-014-7808-6
ISSN: 1618-2642
PubMed: 24752695
WoS: 000337787400018
Scopus: 2-s2.0-84903774678
Институција/група
PharmacyTY - JOUR AU - Jovanović, Marko AU - Rakić, Tijana AU - Jančić-Stojanović, Biljana AU - Ivanović, Darko AU - Medenica, Mirjana PY - 2014 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2191 AB - Hydrophilic interaction liquid chromatography (HILIC) has emerged in recent years as a valuable alternative to reversed-phase liquid chromatography in the analysis of polar compounds. Research in HILIC is divided into two directions: the assessment of the retention mechanism and retention behavior, and the development of HILIC methods. In this work, four polar neutral analytes (iohexol and its related compounds A, B, and C) were analyzed on two silica and two diol columns in HILIC mode with the aim to investigate thoroughly the retention mechanisms and retention behavior of polar neutral compounds on these four columns. The adsorption and partition contribution to the overall HILIC retention mechanism was investigated by fitting the retention data to linear (adsorption and partition) and nonlinear (mixed-retention and quadratic) theoretical models. On the other hand, the establishment of empirical second-order polynomial retention models on the basis of D-optimal design made possible the estimation of the simultaneous influence of several mobile-phase-related factors. Furthermore, these models were also used as the basis for the application of indirect modeling of the selectivity factor and a grid point search approach in order to achieve the optimal separation of analytes. After the optimization goals had been set, the grids were searched and the optimal conditions were identified. Finally, the optimized method was subjected to validation. PB - Springer Heidelberg, Heidelberg T2 - Analytical and Bioanalytical Chemistry T1 - Retention mechanism assessment and method development for the analysis of iohexol and its related compounds in hydrophilic interaction liquid chromatography VL - 406 IS - 17 SP - 4217 EP - 4232 DO - 10.1007/s00216-014-7808-6 ER -
@article{ author = "Jovanović, Marko and Rakić, Tijana and Jančić-Stojanović, Biljana and Ivanović, Darko and Medenica, Mirjana", year = "2014", abstract = "Hydrophilic interaction liquid chromatography (HILIC) has emerged in recent years as a valuable alternative to reversed-phase liquid chromatography in the analysis of polar compounds. Research in HILIC is divided into two directions: the assessment of the retention mechanism and retention behavior, and the development of HILIC methods. In this work, four polar neutral analytes (iohexol and its related compounds A, B, and C) were analyzed on two silica and two diol columns in HILIC mode with the aim to investigate thoroughly the retention mechanisms and retention behavior of polar neutral compounds on these four columns. The adsorption and partition contribution to the overall HILIC retention mechanism was investigated by fitting the retention data to linear (adsorption and partition) and nonlinear (mixed-retention and quadratic) theoretical models. On the other hand, the establishment of empirical second-order polynomial retention models on the basis of D-optimal design made possible the estimation of the simultaneous influence of several mobile-phase-related factors. Furthermore, these models were also used as the basis for the application of indirect modeling of the selectivity factor and a grid point search approach in order to achieve the optimal separation of analytes. After the optimization goals had been set, the grids were searched and the optimal conditions were identified. Finally, the optimized method was subjected to validation.", publisher = "Springer Heidelberg, Heidelberg", journal = "Analytical and Bioanalytical Chemistry", title = "Retention mechanism assessment and method development for the analysis of iohexol and its related compounds in hydrophilic interaction liquid chromatography", volume = "406", number = "17", pages = "4217-4232", doi = "10.1007/s00216-014-7808-6" }
Jovanović, M., Rakić, T., Jančić-Stojanović, B., Ivanović, D.,& Medenica, M.. (2014). Retention mechanism assessment and method development for the analysis of iohexol and its related compounds in hydrophilic interaction liquid chromatography. in Analytical and Bioanalytical Chemistry Springer Heidelberg, Heidelberg., 406(17), 4217-4232. https://doi.org/10.1007/s00216-014-7808-6
Jovanović M, Rakić T, Jančić-Stojanović B, Ivanović D, Medenica M. Retention mechanism assessment and method development for the analysis of iohexol and its related compounds in hydrophilic interaction liquid chromatography. in Analytical and Bioanalytical Chemistry. 2014;406(17):4217-4232. doi:10.1007/s00216-014-7808-6 .
Jovanović, Marko, Rakić, Tijana, Jančić-Stojanović, Biljana, Ivanović, Darko, Medenica, Mirjana, "Retention mechanism assessment and method development for the analysis of iohexol and its related compounds in hydrophilic interaction liquid chromatography" in Analytical and Bioanalytical Chemistry, 406, no. 17 (2014):4217-4232, https://doi.org/10.1007/s00216-014-7808-6 . .