dc.creator | Spasojević-Kalimanovska, Vesna | |
dc.creator | Kalimanovska-Oštrić, Dimitra | |
dc.creator | Jelić-Ivanović, Zorana | |
dc.creator | Topić, Aleksandra | |
dc.creator | Stanković, S | |
dc.creator | Stanojević, N | |
dc.date.accessioned | 2019-09-02T10:51:29Z | |
dc.date.available | 2019-09-02T10:51:29Z | |
dc.date.issued | 1999 | |
dc.identifier.issn | 0354-3447 | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/220 | |
dc.description.abstract | Apolipoprotein E is an important genetic determinant of serum lipoprotein concentrations and coronary artery disease risk. The apo E polymorphism is a major determinant of interindividual variation in the initiation and progression of atherosclerosis. Differences in the gene sequence cause structural changes strongly affecting binding properties of the corresponding apo E isoforms. The presence of the epsilon 2 allele leads to LDL receptor upregulation and increased LDL clearance. In contrast the presence of the epsilon 4 allele leads to receptor down regulation and decreased LDL clearance. For most populations, individuals with the apo epsilon 2 allele display lower levels of plasma cholesterol compared with individuals carrying the apo epsilon 3 allele, whereas individuals with the apo epsilon 4 allele show higher levels of plasma cholesterol, especially LDL-cholesterol and an increased risk for cardiovascular disease. ApoE polymorphism explains near 7% of the variation in total, LDL cholesterol and apo B levels. To examine whether the polymorphism in the apo E gene is associated with CAD in our population, we analyzed 94 patients with angiographically verified CAD and in 45 patients with excluded CAD as a control group. The apo E phenotype was determined with isoelectric Focusing and Western immunoblotting technique using commercial antibodies. Patients were divided into three groups according to arteriograms as category: 1. mild, evidence of narrowing of one coronary artery >60% (39); 2. moderate, narrowing of two major coronary arteries (30) and 3. Severe, narrowing of all three major coronary arteries (25). The apo E allele frequencies of patients with CAD vs without CAD were 4.2% vs 7.7% For epsilon 2; 86.7% vs 85.5% for epsilon 3 and 9.1% vs 6.6%; for epsilon 4. The frequency of allele epsilon 4 was higher in patients with 3 vessel CAD than in patients without CAD (12% vs 6.6%) but it did not reach the statistical significance (chi(2)= 0.83, p = 0.36, d.f. 1). The Odds ratio for risk of CAD associated with the presence of apo epsilon 4 allele was 1.39 and for the phenotype E3/4 1.60. Our preliminary results suggest that the presence of apo epsilon 4 allele may impose some risk for developing CAD and that apo E determines the severity of CAD. | en |
dc.publisher | Društvo medicinskih biohemičara Srbije i Crne Gore, Beograd i Univerzitet u Beogradu - Farmaceutski fakultet, Beograd | |
dc.rights | restrictedAccess | |
dc.source | Jugoslovenska medicinska biohemija | |
dc.subject | Allele frequency | en |
dc.subject | Apolipoprotein E polymorphism | en |
dc.subject | Atherogenic allele | en |
dc.subject | Markers of coronary artery disease | en |
dc.subject | Odds ratio | en |
dc.title | Apolipoprotein E polymorphism and severity of angiographically verified coronary artery disease | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Јелић-Ивановић, Зорана; Топић, A; Станојевић, Н; Станковић, С; Спасојевић-Калимановска, Весна; Калимановска-Оштрић, Димитра; | |
dc.citation.volume | 18 | |
dc.citation.issue | 2-3 | |
dc.citation.spage | 99 | |
dc.citation.epage | 105 | |
dc.citation.other | 18(2-3): 99-105 | |
dc.citation.rank | M23 | |
dc.identifier.wos | 000081375900007 | |
dc.identifier.scopus | 2-s2.0-0032769978 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_farfar_220 | |
dc.type.version | publishedVersion | |