Phenolic profile, antibacterial, antimutagenic and antitumour evaluation of Veronica urticifolia Jacq.
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Barreira, Joao C. M.
Ferreira, Isabel C. F. R.
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The phenolic profile and antibacterial, antimutagenic and antitumour activities of the methanolic extracts of Veronica urticifolia Jacq. were evaluated. HPLC-DAD/ESI-MS analysis revealed the presence of phenolic acids, flavonoids and phenylethanoids, with acteoside as the main component (14.9 mg/g of extract). Antibacterial effect was determined using the microbroth dilution assay and Staphylococcus aureus was the most sensitive strain (MIC and MBC = 7.5 mg/mL). Antimutagenic activity was evaluated by Ames mutagenicity assay. At 1 mg/plate, the tested extract afforded high protection against the mutagenicity of nitroquinoline-N-oxide (4NQO) to Salmonella typhimurium strain TA100 (inhibition rate 48.3%). Antitumour activity was screened in Ehrlich ascites carcinoma (EAC) model. Pretreatment with 2 mg/kg body weight showed statistically significant decrease in tumour cell viability, while ascites volume and tumour cell count became slightly decreased, but not to a statistically significa...nt extent. The results indicate that V. urticifolia deserves further research into its potential chemoprevention effects.
Keywords:Veronica urticifolia / Phenolic profile / HPLC-DAD/ESI-MS / Antibacterial / Antimutagenicity / Ehrlich carcinoma
Source:Journal of Functional Foods, 2014, 9, 192-201
- Elsevier Science BV, Amsterdam
- Biotechnology in vitro - crop, medicinal and endangered plant species (RS-173015)