Vigabatrin in dried plasma spots: Validation of a novel LC-MS/MS method and application to clinical practice
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2014
Authors
Kostić, NadaDotsikas, Yannis

Jović, Nebojša
Stevanović, Galina
Malenović, Anđelija

Medenica, Mirjana
Article (Published version)

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This paper presents a LC-MS/MS method for the determination of antiepileptic drug vigabatrin in dried plasma spots (DPS). Due to its zwitterionic chemical structure, a pre-column derivatization procedure was performed, aiming to yield enhanced ionization efficiency and improved chromatographic behaviour. Propyl chloroformate, in the presence of propanol, was selected as the best derivatization reagent, providing a strong signal along with reasonable run time. A relatively novel sample collection technique, DPS, was utilized, offering easy sample handling and analysis, using a sample in micro amount (similar to 5 mu L). Derivatized vigabatrin and its internal standard, 4-aminocyclohexanecarboxylic acid, were extracted by liquid-liquid extraction (LLE) and determined in positive ion mode by applying two SRM transitions per analyte. A Zorbax Eclipse XDB-C8 column (150 x 4.6 mm, 5 mu m particle size) maintained at 30 degrees C, was utilized with running mobile phase composed of acetonitril...e: 0.15% formic acid (85:15, v/v). Flow rate was 550 mu L/min and total run time 4.5 min. The assay exhibited excellent linearity over the concentration range of 0.500-50.0 mu g/mL, which is suitable for the determination of vigabatrin level after per os administration in children and youths with epilepsy, who were on vigabatrin therapy, with or without co-medication. Specificity, accuracy, precision, recovery, matrix-effect and stability were also estimated and assessed within acceptance criteria.
Keywords:
Vigabatrin / Chloroformates / Dried plasma spots / Mass spectrometrySource:
Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2014, 962, 102-108Publisher:
- Elsevier Science BV, Amsterdam
Funding / projects:
- Modelling of different chromatographic systems with chemometrical approach in pharmaceutical analysis (RS-172052)
DOI: 10.1016/j.jchromb.2014.05.037
ISSN: 1570-0232
PubMed: 24908379
WoS: 000338406800015
Scopus: 2-s2.0-84902007536
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Institution/Community
PharmacyTY - JOUR AU - Kostić, Nada AU - Dotsikas, Yannis AU - Jović, Nebojša AU - Stevanović, Galina AU - Malenović, Anđelija AU - Medenica, Mirjana PY - 2014 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2212 AB - This paper presents a LC-MS/MS method for the determination of antiepileptic drug vigabatrin in dried plasma spots (DPS). Due to its zwitterionic chemical structure, a pre-column derivatization procedure was performed, aiming to yield enhanced ionization efficiency and improved chromatographic behaviour. Propyl chloroformate, in the presence of propanol, was selected as the best derivatization reagent, providing a strong signal along with reasonable run time. A relatively novel sample collection technique, DPS, was utilized, offering easy sample handling and analysis, using a sample in micro amount (similar to 5 mu L). Derivatized vigabatrin and its internal standard, 4-aminocyclohexanecarboxylic acid, were extracted by liquid-liquid extraction (LLE) and determined in positive ion mode by applying two SRM transitions per analyte. A Zorbax Eclipse XDB-C8 column (150 x 4.6 mm, 5 mu m particle size) maintained at 30 degrees C, was utilized with running mobile phase composed of acetonitrile: 0.15% formic acid (85:15, v/v). Flow rate was 550 mu L/min and total run time 4.5 min. The assay exhibited excellent linearity over the concentration range of 0.500-50.0 mu g/mL, which is suitable for the determination of vigabatrin level after per os administration in children and youths with epilepsy, who were on vigabatrin therapy, with or without co-medication. Specificity, accuracy, precision, recovery, matrix-effect and stability were also estimated and assessed within acceptance criteria. PB - Elsevier Science BV, Amsterdam T2 - Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences T1 - Vigabatrin in dried plasma spots: Validation of a novel LC-MS/MS method and application to clinical practice VL - 962 SP - 102 EP - 108 DO - 10.1016/j.jchromb.2014.05.037 ER -
@article{ author = "Kostić, Nada and Dotsikas, Yannis and Jović, Nebojša and Stevanović, Galina and Malenović, Anđelija and Medenica, Mirjana", year = "2014", abstract = "This paper presents a LC-MS/MS method for the determination of antiepileptic drug vigabatrin in dried plasma spots (DPS). Due to its zwitterionic chemical structure, a pre-column derivatization procedure was performed, aiming to yield enhanced ionization efficiency and improved chromatographic behaviour. Propyl chloroformate, in the presence of propanol, was selected as the best derivatization reagent, providing a strong signal along with reasonable run time. A relatively novel sample collection technique, DPS, was utilized, offering easy sample handling and analysis, using a sample in micro amount (similar to 5 mu L). Derivatized vigabatrin and its internal standard, 4-aminocyclohexanecarboxylic acid, were extracted by liquid-liquid extraction (LLE) and determined in positive ion mode by applying two SRM transitions per analyte. A Zorbax Eclipse XDB-C8 column (150 x 4.6 mm, 5 mu m particle size) maintained at 30 degrees C, was utilized with running mobile phase composed of acetonitrile: 0.15% formic acid (85:15, v/v). Flow rate was 550 mu L/min and total run time 4.5 min. The assay exhibited excellent linearity over the concentration range of 0.500-50.0 mu g/mL, which is suitable for the determination of vigabatrin level after per os administration in children and youths with epilepsy, who were on vigabatrin therapy, with or without co-medication. Specificity, accuracy, precision, recovery, matrix-effect and stability were also estimated and assessed within acceptance criteria.", publisher = "Elsevier Science BV, Amsterdam", journal = "Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences", title = "Vigabatrin in dried plasma spots: Validation of a novel LC-MS/MS method and application to clinical practice", volume = "962", pages = "102-108", doi = "10.1016/j.jchromb.2014.05.037" }
Kostić, N., Dotsikas, Y., Jović, N., Stevanović, G., Malenović, A.,& Medenica, M.. (2014). Vigabatrin in dried plasma spots: Validation of a novel LC-MS/MS method and application to clinical practice. in Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences Elsevier Science BV, Amsterdam., 962, 102-108. https://doi.org/10.1016/j.jchromb.2014.05.037
Kostić N, Dotsikas Y, Jović N, Stevanović G, Malenović A, Medenica M. Vigabatrin in dried plasma spots: Validation of a novel LC-MS/MS method and application to clinical practice. in Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. 2014;962:102-108. doi:10.1016/j.jchromb.2014.05.037 .
Kostić, Nada, Dotsikas, Yannis, Jović, Nebojša, Stevanović, Galina, Malenović, Anđelija, Medenica, Mirjana, "Vigabatrin in dried plasma spots: Validation of a novel LC-MS/MS method and application to clinical practice" in Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 962 (2014):102-108, https://doi.org/10.1016/j.jchromb.2014.05.037 . .