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dc.creatorKostić, Nada
dc.creatorDotsikas, Yannis
dc.creatorJović, Nebojša
dc.creatorStevanović, Galina
dc.creatorMalenović, Anđelija
dc.creatorMedenica, Mirjana
dc.date.accessioned2019-09-02T11:41:52Z
dc.date.available2019-09-02T11:41:52Z
dc.date.issued2014
dc.identifier.issn1570-0232
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/2212
dc.description.abstractThis paper presents a LC-MS/MS method for the determination of antiepileptic drug vigabatrin in dried plasma spots (DPS). Due to its zwitterionic chemical structure, a pre-column derivatization procedure was performed, aiming to yield enhanced ionization efficiency and improved chromatographic behaviour. Propyl chloroformate, in the presence of propanol, was selected as the best derivatization reagent, providing a strong signal along with reasonable run time. A relatively novel sample collection technique, DPS, was utilized, offering easy sample handling and analysis, using a sample in micro amount (similar to 5 mu L). Derivatized vigabatrin and its internal standard, 4-aminocyclohexanecarboxylic acid, were extracted by liquid-liquid extraction (LLE) and determined in positive ion mode by applying two SRM transitions per analyte. A Zorbax Eclipse XDB-C8 column (150 x 4.6 mm, 5 mu m particle size) maintained at 30 degrees C, was utilized with running mobile phase composed of acetonitrile: 0.15% formic acid (85:15, v/v). Flow rate was 550 mu L/min and total run time 4.5 min. The assay exhibited excellent linearity over the concentration range of 0.500-50.0 mu g/mL, which is suitable for the determination of vigabatrin level after per os administration in children and youths with epilepsy, who were on vigabatrin therapy, with or without co-medication. Specificity, accuracy, precision, recovery, matrix-effect and stability were also estimated and assessed within acceptance criteria.en
dc.publisherElsevier Science BV, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172052/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
dc.subjectVigabatrinen
dc.subjectChloroformatesen
dc.subjectDried plasma spotsen
dc.subjectMass spectrometryen
dc.titleVigabatrin in dried plasma spots: Validation of a novel LC-MS/MS method and application to clinical practiceen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractМаленовић, Aнђелија; Јовић, Небојша; Костић, Нада; Стевановић, Галина; Меденица, Мирјана; Дотсикас, Yаннис;
dc.citation.volume962
dc.citation.spage102
dc.citation.epage108
dc.citation.other962: 102-108
dc.citation.rankM21
dc.identifier.wos000338406800015
dc.identifier.doi10.1016/j.jchromb.2014.05.037
dc.identifier.pmid24908379
dc.identifier.scopus2-s2.0-84902007536
dc.identifier.rcubconv_3116
dc.type.versionpublishedVersion


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