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Vigabatrin in dried plasma spots: Validation of a novel LC-MS/MS method and application to clinical practice
dc.creator | Kostić, Nada | |
dc.creator | Dotsikas, Yannis | |
dc.creator | Jović, Nebojša | |
dc.creator | Stevanović, Galina | |
dc.creator | Malenović, Anđelija | |
dc.creator | Medenica, Mirjana | |
dc.date.accessioned | 2019-09-02T11:41:52Z | |
dc.date.available | 2019-09-02T11:41:52Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 1570-0232 | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/2212 | |
dc.description.abstract | This paper presents a LC-MS/MS method for the determination of antiepileptic drug vigabatrin in dried plasma spots (DPS). Due to its zwitterionic chemical structure, a pre-column derivatization procedure was performed, aiming to yield enhanced ionization efficiency and improved chromatographic behaviour. Propyl chloroformate, in the presence of propanol, was selected as the best derivatization reagent, providing a strong signal along with reasonable run time. A relatively novel sample collection technique, DPS, was utilized, offering easy sample handling and analysis, using a sample in micro amount (similar to 5 mu L). Derivatized vigabatrin and its internal standard, 4-aminocyclohexanecarboxylic acid, were extracted by liquid-liquid extraction (LLE) and determined in positive ion mode by applying two SRM transitions per analyte. A Zorbax Eclipse XDB-C8 column (150 x 4.6 mm, 5 mu m particle size) maintained at 30 degrees C, was utilized with running mobile phase composed of acetonitrile: 0.15% formic acid (85:15, v/v). Flow rate was 550 mu L/min and total run time 4.5 min. The assay exhibited excellent linearity over the concentration range of 0.500-50.0 mu g/mL, which is suitable for the determination of vigabatrin level after per os administration in children and youths with epilepsy, who were on vigabatrin therapy, with or without co-medication. Specificity, accuracy, precision, recovery, matrix-effect and stability were also estimated and assessed within acceptance criteria. | en |
dc.publisher | Elsevier Science BV, Amsterdam | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172052/RS// | |
dc.rights | restrictedAccess | |
dc.source | Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences | |
dc.subject | Vigabatrin | en |
dc.subject | Chloroformates | en |
dc.subject | Dried plasma spots | en |
dc.subject | Mass spectrometry | en |
dc.title | Vigabatrin in dried plasma spots: Validation of a novel LC-MS/MS method and application to clinical practice | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Маленовић, Aнђелија; Јовић, Небојша; Костић, Нада; Стевановић, Галина; Меденица, Мирјана; Дотсикас, Yаннис; | |
dc.citation.volume | 962 | |
dc.citation.spage | 102 | |
dc.citation.epage | 108 | |
dc.citation.other | 962: 102-108 | |
dc.citation.rank | M21 | |
dc.identifier.wos | 000338406800015 | |
dc.identifier.doi | 10.1016/j.jchromb.2014.05.037 | |
dc.identifier.pmid | 24908379 | |
dc.identifier.scopus | 2-s2.0-84902007536 | |
dc.type.version | publishedVersion |