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dc.creatorĆirić, Andrija
dc.creatorIvanović, Nevena
dc.creatorCvijović, Milica S.
dc.creatorJelikić-Stankov, Milena
dc.creatorJoksović, Ljubinka
dc.creatorĐurđević, Predrag
dc.date.accessioned2019-09-02T11:41:59Z
dc.date.available2019-09-02T11:41:59Z
dc.date.issued2014
dc.identifier.issn1936-9751
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/2216
dc.description.abstractIn the present work, the rapid RP-HPLC method with UV (DAD) detection for simultaneous quantification of bioflavonoids: quercetin, apigenin, catechin, epicatechin, kaempferol, and luteolin in Brassica oleracea species samples (cauliflower, broccoli, and Brussels sprouts) was developed with the aid of LC-Simulator (ACD LabsA (R) suite) software. A series of extracts obtained with different extraction method were evaluated for antioxidant activity. The optimal conditions for separation and quantification were established after nine scouting runs entered to LC-Simulator software. The optimized separation was achieved on Hypersil GOLD aQ column with isocratic elution and mobile phase composition A:2 % acetic acid in water and B:acetonitrile in 91:9 (v/v %) ratio. The R (s) values were in the range from 2.6 to 8.00, indicating good selectivity of the method. The obtained results generally show good agreement with published data. Low detection limits (0.02-0.055 mu g/mL) were obtained with acceptable recoveries (90-109 %). Total time of analysis was less than 11 min; therefore, the proposed method represents significant improvement over existing methods. Extracts from Brassica vegetables, obtained using different extraction procedures, were studied for their radical scavenging effects. Scavenging of DPPH showed different kinetics at the beginning of the assay period and after 15 min from the initialization of reaction. Different kinetics suggested the presence of polymerized and/or less active antioxidants with different scavenging mechanisms for particular polyphenolic compounds.en
dc.publisherSpringer, New York
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172016/RS//
dc.rightsrestrictedAccess
dc.sourceFood Analytical Methods
dc.subjectComputer optimizationen
dc.subjectBioflavonoiden
dc.subjectBrassica vegetablesen
dc.subjectStandard additionen
dc.subjectAntioxidative activityen
dc.titleChemometric-Assisted Optimization of RP-HPLC Method for Determination of Some Bioflavonoids in Brassica oleracea Species and Their Antioxidative Activityen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractИвановић, Невена; Јоксовић, Љубинка; Ћирић, Aндрија; Јеликић-Станков, Милена; Цвијовић, Милица С.; Ђурђевић, Предраг;
dc.citation.volume7
dc.citation.issue7
dc.citation.spage1387
dc.citation.epage1399
dc.citation.other7(7): 1387-1399
dc.citation.rankM22
dc.identifier.wos000338447800004
dc.identifier.doi10.1007/s12161-013-9761-y
dc.identifier.scopus2-s2.0-84903558407
dc.identifier.rcubconv_3069
dc.type.versionpublishedVersion


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