Comparison of Full Factorial Design, Central Composite Design, and Box-Behnken Design in Chromatographic Method Development for the Determination of Fluconazole and Its Impurities
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2014
Authors
Rakić, TijanaKasagić-Vujanović, Irena

Jovanović, Marko
Jančić-Stojanović, Biljana

Ivanović, Darko
Article (Published version)

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This paper presents the development and optimization of a liquid chromatographic method for the determination of fluconazole and its impurities by experimental design methodology. Four experimental design types were applied: two-level full factorial design, central composite design, Box-Behnken design, and three-level full factorial design. The advantages and drawbacks of each design are described and detailed statistical evaluation of mathematical models was performed. The central composite design and three-level full factorial design created significantly better models comparing to the other methods. As the central composite design requires a smaller number of experiments, its models were used for theoretical examination of experimental space. Multiobjective optimization aiming to achieve maximal separation of all investigated substances and minimal analysis duration was performed by a grid point search. The defined optimal separation was achieved on a C-18 (125mmx4mm, 5 mu m particl...e size) column with a mobile phase consisting of acetonitrile and water (5mM ammonium formate) (15:85, v/v); a column temperature of 25 degrees C; a flow rate of 1.2mLmin(-1); and a detection wavelength of 260nm.
Keywords:
Chemometrics / Experimental design / Fluconazole / Impurities / Liquid chromatographySource:
Analytical Letters, 2014, 47, 8, 1334-1347Publisher:
- Taylor & Francis Inc, Philadelphia
Funding / projects:
- Modelling of different chromatographic systems with chemometrical approach in pharmaceutical analysis (RS-172052)
DOI: 10.1080/00032719.2013.867503
ISSN: 0003-2719
WoS: 000335214700005
Scopus: 2-s2.0-84899735672
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PharmacyTY - JOUR AU - Rakić, Tijana AU - Kasagić-Vujanović, Irena AU - Jovanović, Marko AU - Jančić-Stojanović, Biljana AU - Ivanović, Darko PY - 2014 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2223 AB - This paper presents the development and optimization of a liquid chromatographic method for the determination of fluconazole and its impurities by experimental design methodology. Four experimental design types were applied: two-level full factorial design, central composite design, Box-Behnken design, and three-level full factorial design. The advantages and drawbacks of each design are described and detailed statistical evaluation of mathematical models was performed. The central composite design and three-level full factorial design created significantly better models comparing to the other methods. As the central composite design requires a smaller number of experiments, its models were used for theoretical examination of experimental space. Multiobjective optimization aiming to achieve maximal separation of all investigated substances and minimal analysis duration was performed by a grid point search. The defined optimal separation was achieved on a C-18 (125mmx4mm, 5 mu m particle size) column with a mobile phase consisting of acetonitrile and water (5mM ammonium formate) (15:85, v/v); a column temperature of 25 degrees C; a flow rate of 1.2mLmin(-1); and a detection wavelength of 260nm. PB - Taylor & Francis Inc, Philadelphia T2 - Analytical Letters T1 - Comparison of Full Factorial Design, Central Composite Design, and Box-Behnken Design in Chromatographic Method Development for the Determination of Fluconazole and Its Impurities VL - 47 IS - 8 SP - 1334 EP - 1347 DO - 10.1080/00032719.2013.867503 ER -
@article{ author = "Rakić, Tijana and Kasagić-Vujanović, Irena and Jovanović, Marko and Jančić-Stojanović, Biljana and Ivanović, Darko", year = "2014", abstract = "This paper presents the development and optimization of a liquid chromatographic method for the determination of fluconazole and its impurities by experimental design methodology. Four experimental design types were applied: two-level full factorial design, central composite design, Box-Behnken design, and three-level full factorial design. The advantages and drawbacks of each design are described and detailed statistical evaluation of mathematical models was performed. The central composite design and three-level full factorial design created significantly better models comparing to the other methods. As the central composite design requires a smaller number of experiments, its models were used for theoretical examination of experimental space. Multiobjective optimization aiming to achieve maximal separation of all investigated substances and minimal analysis duration was performed by a grid point search. The defined optimal separation was achieved on a C-18 (125mmx4mm, 5 mu m particle size) column with a mobile phase consisting of acetonitrile and water (5mM ammonium formate) (15:85, v/v); a column temperature of 25 degrees C; a flow rate of 1.2mLmin(-1); and a detection wavelength of 260nm.", publisher = "Taylor & Francis Inc, Philadelphia", journal = "Analytical Letters", title = "Comparison of Full Factorial Design, Central Composite Design, and Box-Behnken Design in Chromatographic Method Development for the Determination of Fluconazole and Its Impurities", volume = "47", number = "8", pages = "1334-1347", doi = "10.1080/00032719.2013.867503" }
Rakić, T., Kasagić-Vujanović, I., Jovanović, M., Jančić-Stojanović, B.,& Ivanović, D.. (2014). Comparison of Full Factorial Design, Central Composite Design, and Box-Behnken Design in Chromatographic Method Development for the Determination of Fluconazole and Its Impurities. in Analytical Letters Taylor & Francis Inc, Philadelphia., 47(8), 1334-1347. https://doi.org/10.1080/00032719.2013.867503
Rakić T, Kasagić-Vujanović I, Jovanović M, Jančić-Stojanović B, Ivanović D. Comparison of Full Factorial Design, Central Composite Design, and Box-Behnken Design in Chromatographic Method Development for the Determination of Fluconazole and Its Impurities. in Analytical Letters. 2014;47(8):1334-1347. doi:10.1080/00032719.2013.867503 .
Rakić, Tijana, Kasagić-Vujanović, Irena, Jovanović, Marko, Jančić-Stojanović, Biljana, Ivanović, Darko, "Comparison of Full Factorial Design, Central Composite Design, and Box-Behnken Design in Chromatographic Method Development for the Determination of Fluconazole and Its Impurities" in Analytical Letters, 47, no. 8 (2014):1334-1347, https://doi.org/10.1080/00032719.2013.867503 . .