Aspects of DryLab® software application in chromatography methods optimization and robustness testing

Abstract

In this review article, aspects of DryLab® software application in liquid chromatography are given. Literature survey shows wide application of software for chromatography method development, optimization, as well as for robustness testing. Earlier versions of DryLab®software enabled the simultaneous monitoring of the impact of two parameters on the selected system response, as an upgraded version of the software provides significantly more opportunities. Namely, the new DryLab®software version enables fulfilling requirements given by Quality by Design (QbD) concept through a systematic approach to the development of chromatographic methods, and the construction of a 3D cube which provides the visualization of a Design Space. In addition, when running liquid chromatography methods on a routine basis software can be a useful tool for optimization process in order to minimize retention times and run time, as well as facilitating the transfer of a method (DryLab® data can be sent electronically). Finally, DryLab®software provides useful information about the method and can be applied to training of young analysts without performing experiments, while saving time, money, and laboratory instruments.

U preglednom radu prikazani su aspekti primene DryLab® softvera u tečnoj hromatografiji. Literaturni pregled pokazuje široku primenu softvera u fazi postavljanja nove hromatografske metode, optimizaciji, kao i u proceni robusnosti metode. Ranije verzije DryLab® softvera omogućavale su istovremeno praćenje uticaja dva faktora na odabrani odgovor sistema, dok unapređene verzije softvera daju značajno veće mogućnosti. Novije verzije DryLab® softvera omogućavaju ispunjavanje zahteva određenih Quality by Design (QbD) konceptom kroz sistematičan prilaz razvoju hromatografske metode, kao i kroz konstruisanje 3D kocke kojom se omogućava vizuelni prikaz Design space-a. Pored toga, u rutinskoj primeni metoda tečne hromatografije softver može biti koristan 'alat' za optimizaciju u cilju skraćenja retencionih vremena i trajanja analize, kao i olakšavanja transfera metoda (podaci dobijeni uz pomoć DryLab®-a se mogu slati elektronskim putem). Na kraju, upotrebom DryLab® softvera dobijaju se korisne informacije o metodi, a uz to može služiti za obučavanje mladih analitičara bez izvođenja eksperimenata, uz istovremenu uštedu vremena, novca i laboratorijske opreme.