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Unopposed Estrogen Supplementation/Progesterone Deficiency in Post-Reproductive Age Affects the Secretory Profile of Resident Macrophages in a Tissue-Specific Manner in the Rat
dc.creator | Stanojević, Stanislava | |
dc.creator | Kovačević-Jovanović, Vesna | |
dc.creator | Dimitrijević, Mirjana | |
dc.creator | Vujić, Vesna | |
dc.creator | Curuvija, Ivana | |
dc.creator | Blagojević, Veljko | |
dc.creator | Leposavić, Gordana | |
dc.date.accessioned | 2019-09-02T11:44:12Z | |
dc.date.available | 2019-09-02T11:44:12Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 1046-7408 | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/2298 | |
dc.description.abstract | Problem The influence of unopposed estrogen replacement/isolated progesterone deficiency on macrophage production of pro-inflammatory/anti-inflammatory mediators in the post-reproductive age was studied. Method of study Considering that in the rats post-ovariectomy the circulating estradiol, but not progesterone level rises to the values in sham-operated controls, 20-month-old rats ovariectomized at the age of 10 months served as an experimental model. Estrogen and progesterone receptor expression, secretion of pro- and anti-inflammatory cytokines, and arginine metabolism end-products were examined in splenic and peritoneal macrophages under basal conditions and following lipopolysaccharide (LPS) stimulation in vitro. Results Almost all peritoneal and a subset of splenic macrophages expressed the intracellular progesterone receptor. Ovariectomy diminished cytokine production by splenic (IL-1 beta) and peritoneal (TNF-alpha, IL-1 beta, IL-10) macrophages and increased the production of IL-10 by splenic and TGF-beta by peritoneal cells under basal conditions. Following LPS stimulation, splenic macrophages from ovariectomized rats produced less TNF-alpha and more IL-10, whereas peritoneal macrophages produced less IL-1 beta and TGF-beta than the corresponding cells from sham-operated rats. Ovariectomy diminished urea production in both subpopulations of LPS-stimulated macrophages. Conclusion Although long-lasting isolated progesterone deficiency in the post-reproductive age differentially affects cytokine production in the macrophages from distinct tissue compartments, in both subpopulations, it impairs the pro- inflammatory/anti-inflammatory cytokine secretory balance. | en |
dc.publisher | Wiley, Hoboken | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175050/RS// | |
dc.rights | restrictedAccess | |
dc.source | American Journal of Reproductive Immunology | |
dc.title | Unopposed Estrogen Supplementation/Progesterone Deficiency in Post-Reproductive Age Affects the Secretory Profile of Resident Macrophages in a Tissue-Specific Manner in the Rat | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Лепосавић, Гордана; Благојевић, Вељко; Димитријевић, Мирјана; Вујић, Весна; Станојевић, Станислава; Ковачевић-Јовановић, Весна; Цурувија, Ивана; | |
dc.citation.volume | 74 | |
dc.citation.issue | 5 | |
dc.citation.spage | 445 | |
dc.citation.epage | 456 | |
dc.citation.other | 74(5): 445-456 | |
dc.citation.rank | M22 | |
dc.identifier.wos | 000364913300007 | |
dc.identifier.doi | 10.1111/aji.12424 | |
dc.identifier.pmid | 26307150 | |
dc.identifier.scopus | 2-s2.0-84944152233 | |
dc.type.version | publishedVersion |