Alterations of the X Chromosome in Lymphocytes of Alzheimer's Disease Patients
Apstrakt
Chromosomal alterations as a sign of genetic instability are a feature of Alzheimer's disease (AD). Assessment of the genetic instability of non-neuronal cells of AD patients may provide a method to diagnose or monitor prognosis of the disease. Considering the importance of X chromosome alterations in the possible etiology of AD females, we used fluorescent in situ hybridization (FISH) for the centromere region of the X chromosome to determine aneuploidy, for a possible correlation with premature centromere division (PCD, X) in lymphocytes of AD females and age-matched controls. In AD patients, our results showed a marked and significant increase in the frequency of the X chromosome aneuploidy comparing with age matched controls (p lt 0.001). Also, a significant difference was detected in the PCD, X frequency between AD females when compared with age matched controls (p lt 0.001). In addition, a strong (R2=0.97, n=20) and significant (p lt 0.001) correlation was found between the frequ...ency of aneuploidy and PCD, X in the AD group. Our results support the view that AD is a generalized systematic disease where PCD is to be considered as a stable sign of disease leading to aneuploidy.
Ključne reči:
Alzheimer's disease / aneuploidy / FISH / peripheral blood lymphocytes / PCD / X chromosomeIzvor:
Current Alzheimer Research, 2015, 12, 10, 990-996Izdavač:
- Bentham Science Publ Ltd, Sharjah
Finansiranje / projekti:
DOI: 10.2174/1567205012666151027124154
ISSN: 1567-2050
PubMed: 26502819
WoS: 000366135300009
Scopus: 2-s2.0-84959457872
Institucija/grupa
PharmacyTY - JOUR AU - Potparević, Biljana AU - Bajić, Vladan AU - Perry, George AU - Živković, Lada PY - 2015 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2308 AB - Chromosomal alterations as a sign of genetic instability are a feature of Alzheimer's disease (AD). Assessment of the genetic instability of non-neuronal cells of AD patients may provide a method to diagnose or monitor prognosis of the disease. Considering the importance of X chromosome alterations in the possible etiology of AD females, we used fluorescent in situ hybridization (FISH) for the centromere region of the X chromosome to determine aneuploidy, for a possible correlation with premature centromere division (PCD, X) in lymphocytes of AD females and age-matched controls. In AD patients, our results showed a marked and significant increase in the frequency of the X chromosome aneuploidy comparing with age matched controls (p lt 0.001). Also, a significant difference was detected in the PCD, X frequency between AD females when compared with age matched controls (p lt 0.001). In addition, a strong (R2=0.97, n=20) and significant (p lt 0.001) correlation was found between the frequency of aneuploidy and PCD, X in the AD group. Our results support the view that AD is a generalized systematic disease where PCD is to be considered as a stable sign of disease leading to aneuploidy. PB - Bentham Science Publ Ltd, Sharjah T2 - Current Alzheimer Research T1 - Alterations of the X Chromosome in Lymphocytes of Alzheimer's Disease Patients VL - 12 IS - 10 SP - 990 EP - 996 DO - 10.2174/1567205012666151027124154 ER -
@article{ author = "Potparević, Biljana and Bajić, Vladan and Perry, George and Živković, Lada", year = "2015", abstract = "Chromosomal alterations as a sign of genetic instability are a feature of Alzheimer's disease (AD). Assessment of the genetic instability of non-neuronal cells of AD patients may provide a method to diagnose or monitor prognosis of the disease. Considering the importance of X chromosome alterations in the possible etiology of AD females, we used fluorescent in situ hybridization (FISH) for the centromere region of the X chromosome to determine aneuploidy, for a possible correlation with premature centromere division (PCD, X) in lymphocytes of AD females and age-matched controls. In AD patients, our results showed a marked and significant increase in the frequency of the X chromosome aneuploidy comparing with age matched controls (p lt 0.001). Also, a significant difference was detected in the PCD, X frequency between AD females when compared with age matched controls (p lt 0.001). In addition, a strong (R2=0.97, n=20) and significant (p lt 0.001) correlation was found between the frequency of aneuploidy and PCD, X in the AD group. Our results support the view that AD is a generalized systematic disease where PCD is to be considered as a stable sign of disease leading to aneuploidy.", publisher = "Bentham Science Publ Ltd, Sharjah", journal = "Current Alzheimer Research", title = "Alterations of the X Chromosome in Lymphocytes of Alzheimer's Disease Patients", volume = "12", number = "10", pages = "990-996", doi = "10.2174/1567205012666151027124154" }
Potparević, B., Bajić, V., Perry, G.,& Živković, L.. (2015). Alterations of the X Chromosome in Lymphocytes of Alzheimer's Disease Patients. in Current Alzheimer Research Bentham Science Publ Ltd, Sharjah., 12(10), 990-996. https://doi.org/10.2174/1567205012666151027124154
Potparević B, Bajić V, Perry G, Živković L. Alterations of the X Chromosome in Lymphocytes of Alzheimer's Disease Patients. in Current Alzheimer Research. 2015;12(10):990-996. doi:10.2174/1567205012666151027124154 .
Potparević, Biljana, Bajić, Vladan, Perry, George, Živković, Lada, "Alterations of the X Chromosome in Lymphocytes of Alzheimer's Disease Patients" in Current Alzheimer Research, 12, no. 10 (2015):990-996, https://doi.org/10.2174/1567205012666151027124154 . .