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dc.creatorArsenović-Ranin, Nevena
dc.creatorKosec, Duško
dc.creatorNacka-Aleksić, Mirjana
dc.creatorPilipović, Ivan
dc.creatorStojić-Vukanić, Zorica
dc.creatorĐikić, Jasmina
dc.creatorBufan, Biljana
dc.creatorLeposavić, Gordana
dc.date.accessioned2019-09-02T11:44:52Z
dc.date.available2019-09-02T11:44:52Z
dc.date.issued2015
dc.identifier.issn1535-3702
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/2325
dc.description.abstractThe study examined the putative role of ovarian hormones in shaping of rat peripheral T-cell compartment during post-reproductive period. In 20-month-old rats ovariectomized (Ox) at the very end of reproductive period, thymic output, cellularity and composition of major TCR alpha beta+peripheral blood lymphocyte and splenocyte subsets were analyzed. Ovariectomy led to the enlargement of CD8 + peripheral blood lymphocyte and splenocyte subpopulations. This reflected: (i) a more efficient thymic generation of CD8 + cells as indicated by increased number of CD4+CD8+double positive and the most mature CD4CD8+TCR alpha beta(high) thymocytes and CD8 + recent thymic emigrants (RTEs) in peripheral blood, but not in the spleen of Ox rats, and (ii) the expansion of CD8 + memory/activated peripheral blood lymphocytes and splenocytes. The latter was consistent with a greater frequency of proliferating cells among freshly isolated memory/activated CD8 + peripheral blood lymphocytes and splenocytes and increased proliferative response of CD8 + splenocytes to stimulation with plate-bound anti-CD3 antibody. The former could be related to the rise in splenic IL-7 and IL-15 mRNA expression. Although ovariectomy affected the overall number of CD4 + T cells in none of the examined compartments, it increased CD4+FoxP3 + peripheral blood lymphocyte and splenocyte counts by enhancing their generation in periphery. Collectively, the results suggest that ovariectomy-induced long-lasting disturbances in ovarian hormone levels (mirrored in diminished progesterone serum level in 20-month-old rats) affects both thymic CD8 + cell generation and peripheral homeostasis and leads to the expansion of CD4+FoxP3 + cells in the periphery, thereby enhancing autoreactive cell control on account of immune system efficacy to combat infections and tumors.en
dc.publisherSage Publications Ltd, London
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175050/RS//
dc.rightsopenAccess
dc.sourceExperimental Biology and Medicine
dc.titleOvarian hormone level alterations during rat post-reproductive life-span influence CD8+T-cell homeostasisen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractНацка-Aлексић, Мирјана; Пилиповић, Иван; Ђикић, Јасмина; Лепосавић, Гордана; Косец, Душко; Aрсеновић-Ранин, Невена; Буфан, Биљана; Стојић-Вуканић, Зорица;
dc.citation.volume240
dc.citation.issue10
dc.citation.spage1319
dc.citation.epage1332
dc.citation.other240(10): 1319-1332
dc.citation.rankM22
dc.identifier.wos000362190300007
dc.identifier.doi10.1177/1535370215570817
dc.identifier.pmid25716018
dc.identifier.scopus2-s2.0-84942844167
dc.identifier.fulltexthttp://farfar.pharmacy.bg.ac.rs//bitstream/id/1037/2323.pdf
dc.identifier.rcubconv_3402
dc.type.versionpublishedVersion


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