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dc.creatorGandhi, Jarel K.
dc.creatorŽivković, Lada
dc.creatorFisher, John P.
dc.creatorYoder, Mervin C.
dc.creatorBrey, Eric M.
dc.date.accessioned2019-09-02T11:44:59Z
dc.date.available2019-09-02T11:44:59Z
dc.date.issued2015
dc.identifier.issn1424-8220
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/2328
dc.description.abstractEnhanced vascularization at sensor interfaces can improve long-term function. Fibrin, a natural polymer, has shown promise as a biomaterial for sensor coating due to its ability to sustain endothelial cell growth and promote local vascularization. However, the culture of cells, particularly endothelial cells (EC), within 3D scaffolds for more than a few days is challenging due to rapid loss of EC viability. In this manuscript, a robust method for developing fibrin microbead scaffolds for long-term culture of encapsulated ECs is described. Fibrin microbeads are formed using sodium alginate as a structural template. The size, swelling and structural properties of the microbeads were varied with needle gauge and composition and concentration of the pre-gel solution. Endothelial colony-forming cells (ECFCs) were suspended in the fibrin beads and cultured within a perfusion bioreactor system. The perfusion bioreactor enhanced ECFCs viability and genome stability in fibrin beads relative to static culture. Perfusion bioreactors enable 3D culture of ECs within fibrin beads for potential application as a sensor coating.en
dc.publisherMDPI, Basel
dc.relationDepartment of Veterans Affairs
dc.relationFulbright Program
dc.rightsopenAccess
dc.sourceSeparation Science and Technology
dc.titleEnhanced Viability of Endothelial Colony Forming Cells in Fibrin Microbeads for Sensor Vascularizationen
dc.typearticle
dc.rights.licenseBY
dcterms.abstractБреy, Ериц М.; Yодер, Мервин Ц.; Живковић, Лада; Фисхер, Јохн П.; Гандхи, Јарел К.;
dc.citation.volume15
dc.citation.issue9
dc.citation.spage23886
dc.citation.epage23902
dc.citation.other15(9): 23886-23902
dc.citation.rankM21
dc.identifier.wos000362512200146
dc.identifier.doi10.3390/s150923886
dc.identifier.pmid26393602
dc.identifier.scopus2-s2.0-85019119230
dc.identifier.fulltexthttp://farfar.pharmacy.bg.ac.rs//bitstream/id/1040/2326.pdf
dc.identifier.rcubconv_3405
dc.type.versionpublishedVersion


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