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Analysis of phenolics in wine by high performance thin-layer chromatography with gradient elution and high resolution plate imaging

Authorized Users Only
2015
Authors
Agatonović-Kuštrin, Snežana
Hettiarachchi, Chandima G.
Morton, David W.
Ražić, Slavica
Article (Published version)
Metadata
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Abstract
Health benefits of wine, especially with red wine, have been linked to the presence of a wide range of phenolic antioxidants. Thus, the aim of this study was to develop a simple, high performance thin layer chromatographic (HPTLC) method combined with high resolution digital plate images to visually compare multiple wine samples simultaneously on a single chromatographic plate and to quantify levels of gallic acid, caffeic acid, resveratrol and rutin, as representatives of the four different classes of phenolics found in wines. We also wanted to investigate the contribution of the investigated phenolic compounds to the total polyphenolic content (TPC) and total antioxidant capacity (TAC) of the wine samples. The average concentrations of caffeic acid, gallic acid, resveratrol, and rutin in the red wines were 2.15, 30.17, 0.59 and 2.47 mg/L respectively with their concentration below limit of quantification in the white wine samples. The highest concentration of resveratrol and rutin is... found in the Cabernet and Shiraz wine samples. The amounts of gallic acid are correlated with TPC (r = 0.58). Italian wines have the highest correlation between TPC and TAC (r = 0.99) although they do not contain detectable amounts of resveratrol, they contain significant amount of rutin. Therefore, antioxidant properties might be associated with the presence of flavanols in these wines.

Keywords:
Wine phenolics / High resolution plate imaging / HPTLC / Gradient elution / AMD chamber
Source:
Journal of Pharmaceutical and Biomedical Analysis, 2015, 102, 93-99
Publisher:
  • Elsevier Science BV, Amsterdam

DOI: 10.1016/j.jpba.2014.08.031

ISSN: 0731-7085

PubMed: 25255450

WoS: 000347277500011

Scopus: 2-s2.0-84907483623
[ Google Scholar ]
30
23
URI
https://farfar.pharmacy.bg.ac.rs/handle/123456789/2381
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Pharmacy
TY  - JOUR
AU  - Agatonović-Kuštrin, Snežana
AU  - Hettiarachchi, Chandima G.
AU  - Morton, David W.
AU  - Ražić, Slavica
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2381
AB  - Health benefits of wine, especially with red wine, have been linked to the presence of a wide range of phenolic antioxidants. Thus, the aim of this study was to develop a simple, high performance thin layer chromatographic (HPTLC) method combined with high resolution digital plate images to visually compare multiple wine samples simultaneously on a single chromatographic plate and to quantify levels of gallic acid, caffeic acid, resveratrol and rutin, as representatives of the four different classes of phenolics found in wines. We also wanted to investigate the contribution of the investigated phenolic compounds to the total polyphenolic content (TPC) and total antioxidant capacity (TAC) of the wine samples. The average concentrations of caffeic acid, gallic acid, resveratrol, and rutin in the red wines were 2.15, 30.17, 0.59 and 2.47 mg/L respectively with their concentration below limit of quantification in the white wine samples. The highest concentration of resveratrol and rutin is found in the Cabernet and Shiraz wine samples. The amounts of gallic acid are correlated with TPC (r = 0.58). Italian wines have the highest correlation between TPC and TAC (r = 0.99) although they do not contain detectable amounts of resveratrol, they contain significant amount of rutin. Therefore, antioxidant properties might be associated with the presence of flavanols in these wines.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Analysis of phenolics in wine by high performance thin-layer chromatography with gradient elution and high resolution plate imaging
VL  - 102
SP  - 93
EP  - 99
DO  - 10.1016/j.jpba.2014.08.031
ER  - 
@article{
author = "Agatonović-Kuštrin, Snežana and Hettiarachchi, Chandima G. and Morton, David W. and Ražić, Slavica",
year = "2015",
abstract = "Health benefits of wine, especially with red wine, have been linked to the presence of a wide range of phenolic antioxidants. Thus, the aim of this study was to develop a simple, high performance thin layer chromatographic (HPTLC) method combined with high resolution digital plate images to visually compare multiple wine samples simultaneously on a single chromatographic plate and to quantify levels of gallic acid, caffeic acid, resveratrol and rutin, as representatives of the four different classes of phenolics found in wines. We also wanted to investigate the contribution of the investigated phenolic compounds to the total polyphenolic content (TPC) and total antioxidant capacity (TAC) of the wine samples. The average concentrations of caffeic acid, gallic acid, resveratrol, and rutin in the red wines were 2.15, 30.17, 0.59 and 2.47 mg/L respectively with their concentration below limit of quantification in the white wine samples. The highest concentration of resveratrol and rutin is found in the Cabernet and Shiraz wine samples. The amounts of gallic acid are correlated with TPC (r = 0.58). Italian wines have the highest correlation between TPC and TAC (r = 0.99) although they do not contain detectable amounts of resveratrol, they contain significant amount of rutin. Therefore, antioxidant properties might be associated with the presence of flavanols in these wines.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Analysis of phenolics in wine by high performance thin-layer chromatography with gradient elution and high resolution plate imaging",
volume = "102",
pages = "93-99",
doi = "10.1016/j.jpba.2014.08.031"
}
Agatonović-Kuštrin, S., Hettiarachchi, C. G., Morton, D. W.,& Ražić, S.. (2015). Analysis of phenolics in wine by high performance thin-layer chromatography with gradient elution and high resolution plate imaging. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier Science BV, Amsterdam., 102, 93-99.
https://doi.org/10.1016/j.jpba.2014.08.031
Agatonović-Kuštrin S, Hettiarachchi CG, Morton DW, Ražić S. Analysis of phenolics in wine by high performance thin-layer chromatography with gradient elution and high resolution plate imaging. in Journal of Pharmaceutical and Biomedical Analysis. 2015;102:93-99.
doi:10.1016/j.jpba.2014.08.031 .
Agatonović-Kuštrin, Snežana, Hettiarachchi, Chandima G., Morton, David W., Ražić, Slavica, "Analysis of phenolics in wine by high performance thin-layer chromatography with gradient elution and high resolution plate imaging" in Journal of Pharmaceutical and Biomedical Analysis, 102 (2015):93-99,
https://doi.org/10.1016/j.jpba.2014.08.031 . .

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