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dc.creatorKasagić-Vujanović, Irena
dc.creatorJančić-Stojanović, Biljana
dc.creatorRakić, Tijana
dc.creatorIvanović, Darko
dc.date.accessioned2019-09-02T11:46:44Z
dc.date.available2019-09-02T11:46:44Z
dc.date.issued2015
dc.identifier.issn1082-6076
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/2392
dc.description.abstractIn this paper chemometrically-assisted optimization and validation of hydrophilic interaction liquid chromatographic (HILIC) method for the quantitative analysis of amlodipine besylate (AB) and bisoprolol fumarate (BF) in pharmaceuticals are presented. In this study, the development of HILIC method for AB and BF separation was performed by a systematic approach which included Design of Experiments (DoE) methodology. First, in method optimization, a central composite design was applied. Influence of the acetonitrile content in the mobile phase, pH of the aqueous phase, and concentration of ammonium acetate on selected chromatographic responses was investigated. Optimal chromatographic conditions in terms of adequate separation and minimal analysis duration were set by graphical optimization overlapping the contour plots as: acetonitrile-water phase (10mM ammonium acetate, pH 4.0 adjusted with glacial acetic acid) (92:8, v/v), column Luna HILIC 200 A, 150mmx4.6mm, 5 mu m particle size, column temperature 30 degrees C, flow rate 1mL min(-1), and UV detection at 230nm. After this step, method robustness was tested using a 2(5-1) fractional factorial design. Statistical and graphical evaluation of results confirmed robustness of the method and then the method was fully validated. Finally, the proposed method was successfully applied for AB and BF determination in pharmaceuticals.en
dc.publisherTaylor & Francis Inc, Philadelphia
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172052/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Liquid Chromatography & Related Technologies
dc.subjectoptimization of methoden
dc.subjectvalidation of methoden
dc.subjectbisoprolol fumarateen
dc.subjectHILICen
dc.subjectamlodipine besylateen
dc.subjectDesign of Experimentsen
dc.titleDesign of Experiments in Optimization and Validation of a Hydrophilic Interaction Liquid Chromatography Method for Determination of Amlodipine Besylate and Bisoprolol Fumarateen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractРакић, Тијана; Ивановић, Дарко; Јанчић-Стојановић, Биљана; Касагић-Вујановић, Ирена;
dc.citation.volume38
dc.citation.issue8
dc.citation.spage919
dc.citation.epage928
dc.citation.other38(8): 919-928
dc.citation.rankM23
dc.identifier.wos000349670100012
dc.identifier.doi10.1080/10826076.2014.991872
dc.identifier.scopus2-s2.0-84923672972
dc.identifier.rcubconv_3270
dc.type.versionpublishedVersion


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