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dc.creatorKostić, Nada
dc.creatorDotsikas, Yannis
dc.creatorJović, Nebojša
dc.creatorStevanović, Galina
dc.creatorMalenović, Anđelija
dc.creatorMedenica, Mirjana
dc.date.accessioned2019-09-02T11:47:15Z
dc.date.available2019-09-02T11:47:15Z
dc.date.issued2015
dc.identifier.issn0731-7085
dc.identifier.urihttps://farfar.pharmacy.bg.ac.rs/handle/123456789/2410
dc.description.abstractIn this paper, novel LC-MS/MS methods for the determination of antiepileptic drug pregabalin in dried matrix spots (DMS) are presented. This attractive technique of sample collection in micro amount was utilized in the form of dried blood spots (DBS) and dried plasma spots (DPS). Following a pre-column derivatization procedure, using n-propyl chloroformate in the presence of n-propanol, and consecutive liquid-liquid extraction, derivatized pregabalin and its internal standard, 4-aminocyclohexanecarboxylic acid, were detected in positive ion mode by applying two SRM transitions per analyte. A YMC-Pack Octyl column (50 mm x 4.0 mm, 3 mu m particle size) maintained at 30 degrees C, was utilized with running mobile phase composed of acetonitrile: 0.15% formic acid (85:15, v/v). Flow rate was 550 mu L/min and total run time 2 min. Established methods were fully validated over the concentration range of 0.200-20.0 mu g/mL for DBS and 0.400-40.0 mu g/mL for DPS, respectively, while specificity, accuracy, precision, recovery, matrix-effect, stability, dilution integrity and spot homogeneity were found within acceptance criteria. Validated methods were applied for the determination of pregabalin levels in dried blood and plasma samples obtained from patients with epilepsy, after per os administration of commercial capsules. Comparison of drug level in blood and plasma, as well as correction steps undertaken in order to overcome hematocrit issue, when analyzing DBS, are also given.en
dc.publisherElsevier Science BV, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172052/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Pharmaceutical and Biomedical Analysis
dc.subjectPregabalinen
dc.subjectChloroformatesen
dc.subjectDried blood/plasma spotsen
dc.subjectMass spectrometryen
dc.subjectHematocriten
dc.titleQuantitation of pregabalin in dried blood spots and dried plasma spots by validated LC-MS/MS methodsen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractМеденица, Мирјана; Јовић, Небојша; Маленовић, Aнђелија; Костић, Нада; Дотсикас, Yаннис; Стевановић, Галина;
dc.citation.volume109
dc.citation.spage79
dc.citation.epage84
dc.citation.other109: 79-84
dc.citation.rankM21
dc.identifier.wos000353078700011
dc.identifier.doi10.1016/j.jpba.2015.02.023
dc.identifier.pmid25767905
dc.identifier.scopus2-s2.0-84924370331
dc.type.versionpublishedVersion


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Приказ основних података о документу