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Evaluation of cytogenetic and DNA damage in human lymphocytes treated with adrenaline in vitro

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2015
Authors
Đelić, Ninoslav
Radaković, Milena
Potparević, Biljana
Živković, Lada
Bajić, Vladan
Stevanović, Jevrosima
Stanimirović, Zoran
Article (Published version)
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Abstract
Catechol groups can be involved in redox cycling accompanied by generation of reactive oxygen species (ROS) which may lead to oxidative damage of cellular macromolecules including DNA. The objective of this investigation was to evaluate possible genotoxic effects of a natural catecholamine adrenaline in cultured human lymphocytes using cytogenetic (sister chromatid exchange and micronuclei) and the single cell gel electrophoresis (Comet) assay. In cytogenetic tests, six experimental concentrations of adrenaline were used in a range from 0.01-500 mu M. There were no indications of genotoxic effects of adrenaline in sister chromatid exchange and micronucleus tests. However, at four highest concentrations of adrenaline (5 mu M, 50 mu M, 150 mu M and 300 mu M) we observed a decreased mitotic index and cell-cycle delay. In addition, in the Comet assay we used adrenaline in a range from 0.0005-500 mu M, at two treatment times: 15 min or 60 min. In contrast to cytogenetic analysis, there was ...a dose-dependent increase of DNA damage detected in the Comet assay. These effects were significantly reduced by concomitant treatment with quercetin or catalase. Therefore, the obtained results indicate that adrenaline may exhibit genotoxic effects in cultured human lymphocytes, most likely due to production of reactive oxygen species.

Keywords:
Sister chromatid exchange / Micronuclei / DNA damage / Oxidative stress
Source:
Toxicology Letters, 2015, 29, 1, 27-33
Publisher:
  • Pergamon-Elsevier Science Ltd, Oxford
Projects:
  • Molecular genetic and ecophysiological researches on the protection of autochthonous animal genetic resources, sustaining domestic animals’ welfare, health and reproduction, and safe food production (RS-46002)

DOI: 10.1016/j.tiv.2014.08.001

ISSN: 0887-2333

PubMed: 25172298

WoS: 000347746600004

Scopus: 2-s2.0-84907661875
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URI
http://farfar.pharmacy.bg.ac.rs/handle/123456789/2411
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  • Radovi istraživača / Researchers’ publications
Institution
Pharmacy

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