Catechol groups can be involved in redox cycling accompanied by generation of reactive oxygen species (ROS) which may lead to oxidative damage of cellular macromolecules including DNA. The objective of this investigation was to evaluate possible genotoxic effects of a natural catecholamine adrenaline in cultured human lymphocytes using cytogenetic (sister chromatid exchange and micronuclei) and the single cell gel electrophoresis (Comet) assay. In cytogenetic tests, six experimental concentrations of adrenaline were used in a range from 0.01-500 mu M. There were no indications of genotoxic effects of adrenaline in sister chromatid exchange and micronucleus tests. However, at four highest concentrations of adrenaline (5 mu M, 50 mu M, 150 mu M and 300 mu M) we observed a decreased mitotic index and cell-cycle delay. In addition, in the Comet assay we used adrenaline in a range from 0.0005-500 mu M, at two treatment times: 15 min or 60 min. In contrast to cytogenetic analysis, there was ...a dose-dependent increase of DNA damage detected in the Comet assay. These effects were significantly reduced by concomitant treatment with quercetin or catalase. Therefore, the obtained results indicate that adrenaline may exhibit genotoxic effects in cultured human lymphocytes, most likely due to production of reactive oxygen species.
TY - JOUR
AU - Đelić, Ninoslav
AU - Radaković, Milena
AU - Potparević, Biljana
AU - Živković, Lada
AU - Bajić, Vladan
AU - Stevanović, Jevrosima
AU - Stanimirović, Zoran
PY - 2015
UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2411
AB - Catechol groups can be involved in redox cycling accompanied by generation of reactive oxygen species (ROS) which may lead to oxidative damage of cellular macromolecules including DNA. The objective of this investigation was to evaluate possible genotoxic effects of a natural catecholamine adrenaline in cultured human lymphocytes using cytogenetic (sister chromatid exchange and micronuclei) and the single cell gel electrophoresis (Comet) assay. In cytogenetic tests, six experimental concentrations of adrenaline were used in a range from 0.01-500 mu M. There were no indications of genotoxic effects of adrenaline in sister chromatid exchange and micronucleus tests. However, at four highest concentrations of adrenaline (5 mu M, 50 mu M, 150 mu M and 300 mu M) we observed a decreased mitotic index and cell-cycle delay. In addition, in the Comet assay we used adrenaline in a range from 0.0005-500 mu M, at two treatment times: 15 min or 60 min. In contrast to cytogenetic analysis, there was a dose-dependent increase of DNA damage detected in the Comet assay. These effects were significantly reduced by concomitant treatment with quercetin or catalase. Therefore, the obtained results indicate that adrenaline may exhibit genotoxic effects in cultured human lymphocytes, most likely due to production of reactive oxygen species.
PB - Pergamon-Elsevier Science Ltd, Oxford
T2 - Toxicology Letters
T1 - Evaluation of cytogenetic and DNA damage in human lymphocytes treated with adrenaline in vitro
VL - 29
IS - 1
SP - 27
EP - 33
DO - 10.1016/j.tiv.2014.08.001
ER -
@article{
author = "Đelić, Ninoslav and Radaković, Milena and Potparević, Biljana and Živković, Lada and Bajić, Vladan and Stevanović, Jevrosima and Stanimirović, Zoran",
year = "2015",
abstract = "Catechol groups can be involved in redox cycling accompanied by generation of reactive oxygen species (ROS) which may lead to oxidative damage of cellular macromolecules including DNA. The objective of this investigation was to evaluate possible genotoxic effects of a natural catecholamine adrenaline in cultured human lymphocytes using cytogenetic (sister chromatid exchange and micronuclei) and the single cell gel electrophoresis (Comet) assay. In cytogenetic tests, six experimental concentrations of adrenaline were used in a range from 0.01-500 mu M. There were no indications of genotoxic effects of adrenaline in sister chromatid exchange and micronucleus tests. However, at four highest concentrations of adrenaline (5 mu M, 50 mu M, 150 mu M and 300 mu M) we observed a decreased mitotic index and cell-cycle delay. In addition, in the Comet assay we used adrenaline in a range from 0.0005-500 mu M, at two treatment times: 15 min or 60 min. In contrast to cytogenetic analysis, there was a dose-dependent increase of DNA damage detected in the Comet assay. These effects were significantly reduced by concomitant treatment with quercetin or catalase. Therefore, the obtained results indicate that adrenaline may exhibit genotoxic effects in cultured human lymphocytes, most likely due to production of reactive oxygen species.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Toxicology Letters",
title = "Evaluation of cytogenetic and DNA damage in human lymphocytes treated with adrenaline in vitro",
volume = "29",
number = "1",
pages = "27-33",
doi = "10.1016/j.tiv.2014.08.001"
}
Đelić, N., Radaković, M., Potparević, B., Živković, L., Bajić, V., Stevanović, J.,& Stanimirović, Z.. (2015). Evaluation of cytogenetic and DNA damage in human lymphocytes treated with adrenaline in vitro. in Toxicology Letters
Pergamon-Elsevier Science Ltd, Oxford., 29(1), 27-33.
https://doi.org/10.1016/j.tiv.2014.08.001
Đelić N, Radaković M, Potparević B, Živković L, Bajić V, Stevanović J, Stanimirović Z. Evaluation of cytogenetic and DNA damage in human lymphocytes treated with adrenaline in vitro. in Toxicology Letters. 2015;29(1):27-33.
doi:10.1016/j.tiv.2014.08.001 .
Đelić, Ninoslav, Radaković, Milena, Potparević, Biljana, Živković, Lada, Bajić, Vladan, Stevanović, Jevrosima, Stanimirović, Zoran, "Evaluation of cytogenetic and DNA damage in human lymphocytes treated with adrenaline in vitro" in Toxicology Letters, 29, no. 1 (2015):27-33,
https://doi.org/10.1016/j.tiv.2014.08.001 . .
