FarFaR - Pharmacy Repository
University of Belgrade, Faculty of Pharmacy
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrilic)
    • Serbian (Latin)
  • Login
View Item 
  •   FarFaR
  • Pharmacy
  • Radovi istraživača / Researchers’ publications
  • View Item
  •   FarFaR
  • Pharmacy
  • Radovi istraživača / Researchers’ publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Optimisation of isolation procedure for pyrrolizidine alkaloids from Rindera umbellata Bunge

Authorized Users Only
2015
Authors
Mandić, Boris M.
Vlajić, Marina D.
Trifunović, Snežana
Simić, Milena
Vujisić, Ljubodrag V.
Vucković, Ivan M.
Novaković, Miroslav
Nikolić-Mandić, Snežana D.
Tešević, Vele
Vajs, Vlatka
Milosavljević, Slobodan
Article (Published version)
Metadata
Show full item record
Abstract
Procedure for isolation of pyrrolizidine alkaloids (PAs) from Rindera umbellata Bunge plant species was optimised. Different extraction media (methanol, ethanol and sulphuric acid), concentration and volume of sulphuric acid, pH of PA solution for alkaline extraction, extraction time and techniques (maceration, ultrasonic and overhead rotary mixer assisted extraction) were investigated. The yields of six PAs (7-angeloyl heliotridane, 7-angeloyl heliotridine, lindelofine, 7-angeloyl rinderine, punctanecine and heliosupine) were monitored by GC-MS/FID. The best results for the isolation all of six PAs were obtained when the extraction was performed with 1M sulphuric acid (30mL per 1.00g of dried sample) by overhead rotary mixer during three days. Optimal pH value for alkaline extraction of PAs with CH2Cl2 was 9, and the extraction should be performed with four portions of 30mL of CH2Cl2. This procedure could be also useful for a plant sample preparation for GC and LC analyses of PAs.
Keywords:
isolation / GC-MS / pyrrolizidine alkaloids / Rindera umellata Bunge
Source:
Natural Product Research, 2015, 29, 9, 887-890
Publisher:
  • Taylor & Francis Ltd, Abingdon
Projects:
  • Natural products of wild, cultivated and edible plants: structure and bioactivity determination (RS-172053)

DOI: 10.1080/14786419.2014.991929

ISSN: 1478-6419

PubMed: 25528897

WoS: 000352853400019

Scopus: 2-s2.0-84933044367
[ Google Scholar ]
5
5
URI
http://farfar.pharmacy.bg.ac.rs/handle/123456789/2412
Collections
  • Radovi istraživača / Researchers’ publications
Institution
Pharmacy

DSpace software copyright © 2002-2015  DuraSpace
About FarFaR - Pharmacy Repository | Send Feedback

OpenAIRERCUB
 

 

All of DSpaceInstitutionsAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About FarFaR - Pharmacy Repository | Send Feedback

OpenAIRERCUB