Optimisation of isolation procedure for pyrrolizidine alkaloids from Rindera umbellata Bunge
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2015
Authors
Mandić, Boris M.
Vlajić, Marina D.
Trifunović, Snežana

Simić, Milena

Vujisić, Ljubodrag V.

Vucković, Ivan M.
Novaković, Miroslav
Nikolić-Mandić, Snežana D.
Tešević, Vele

Vajs, Vlatka
Milosavljević, Slobodan
Article (Published version)

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Procedure for isolation of pyrrolizidine alkaloids (PAs) from Rindera umbellata Bunge plant species was optimised. Different extraction media (methanol, ethanol and sulphuric acid), concentration and volume of sulphuric acid, pH of PA solution for alkaline extraction, extraction time and techniques (maceration, ultrasonic and overhead rotary mixer assisted extraction) were investigated. The yields of six PAs (7-angeloyl heliotridane, 7-angeloyl heliotridine, lindelofine, 7-angeloyl rinderine, punctanecine and heliosupine) were monitored by GC-MS/FID. The best results for the isolation all of six PAs were obtained when the extraction was performed with 1M sulphuric acid (30mL per 1.00g of dried sample) by overhead rotary mixer during three days. Optimal pH value for alkaline extraction of PAs with CH2Cl2 was 9, and the extraction should be performed with four portions of 30mL of CH2Cl2. This procedure could be also useful for a plant sample preparation for GC and LC analyses of PAs.
Keywords:
isolation / GC-MS / pyrrolizidine alkaloids / Rindera umellata BungeSource:
Natural Product Research, 2015, 29, 9, 887-890Publisher:
- Taylor & Francis Ltd, Abingdon
Funding / projects:
- Natural products of wild, cultivated and edible plants: structure and bioactivity determination (RS-172053)
DOI: 10.1080/14786419.2014.991929
ISSN: 1478-6419
PubMed: 25528897
WoS: 000352853400019
Scopus: 2-s2.0-84933044367
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Institution/Community
PharmacyTY - JOUR AU - Mandić, Boris M. AU - Vlajić, Marina D. AU - Trifunović, Snežana AU - Simić, Milena AU - Vujisić, Ljubodrag V. AU - Vucković, Ivan M. AU - Novaković, Miroslav AU - Nikolić-Mandić, Snežana D. AU - Tešević, Vele AU - Vajs, Vlatka AU - Milosavljević, Slobodan PY - 2015 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2412 AB - Procedure for isolation of pyrrolizidine alkaloids (PAs) from Rindera umbellata Bunge plant species was optimised. Different extraction media (methanol, ethanol and sulphuric acid), concentration and volume of sulphuric acid, pH of PA solution for alkaline extraction, extraction time and techniques (maceration, ultrasonic and overhead rotary mixer assisted extraction) were investigated. The yields of six PAs (7-angeloyl heliotridane, 7-angeloyl heliotridine, lindelofine, 7-angeloyl rinderine, punctanecine and heliosupine) were monitored by GC-MS/FID. The best results for the isolation all of six PAs were obtained when the extraction was performed with 1M sulphuric acid (30mL per 1.00g of dried sample) by overhead rotary mixer during three days. Optimal pH value for alkaline extraction of PAs with CH2Cl2 was 9, and the extraction should be performed with four portions of 30mL of CH2Cl2. This procedure could be also useful for a plant sample preparation for GC and LC analyses of PAs. PB - Taylor & Francis Ltd, Abingdon T2 - Natural Product Research T1 - Optimisation of isolation procedure for pyrrolizidine alkaloids from Rindera umbellata Bunge VL - 29 IS - 9 SP - 887 EP - 890 DO - 10.1080/14786419.2014.991929 ER -
@article{ author = "Mandić, Boris M. and Vlajić, Marina D. and Trifunović, Snežana and Simić, Milena and Vujisić, Ljubodrag V. and Vucković, Ivan M. and Novaković, Miroslav and Nikolić-Mandić, Snežana D. and Tešević, Vele and Vajs, Vlatka and Milosavljević, Slobodan", year = "2015", abstract = "Procedure for isolation of pyrrolizidine alkaloids (PAs) from Rindera umbellata Bunge plant species was optimised. Different extraction media (methanol, ethanol and sulphuric acid), concentration and volume of sulphuric acid, pH of PA solution for alkaline extraction, extraction time and techniques (maceration, ultrasonic and overhead rotary mixer assisted extraction) were investigated. The yields of six PAs (7-angeloyl heliotridane, 7-angeloyl heliotridine, lindelofine, 7-angeloyl rinderine, punctanecine and heliosupine) were monitored by GC-MS/FID. The best results for the isolation all of six PAs were obtained when the extraction was performed with 1M sulphuric acid (30mL per 1.00g of dried sample) by overhead rotary mixer during three days. Optimal pH value for alkaline extraction of PAs with CH2Cl2 was 9, and the extraction should be performed with four portions of 30mL of CH2Cl2. This procedure could be also useful for a plant sample preparation for GC and LC analyses of PAs.", publisher = "Taylor & Francis Ltd, Abingdon", journal = "Natural Product Research", title = "Optimisation of isolation procedure for pyrrolizidine alkaloids from Rindera umbellata Bunge", volume = "29", number = "9", pages = "887-890", doi = "10.1080/14786419.2014.991929" }
Mandić, B. M., Vlajić, M. D., Trifunović, S., Simić, M., Vujisić, L. V., Vucković, I. M., Novaković, M., Nikolić-Mandić, S. D., Tešević, V., Vajs, V.,& Milosavljević, S.. (2015). Optimisation of isolation procedure for pyrrolizidine alkaloids from Rindera umbellata Bunge. in Natural Product Research Taylor & Francis Ltd, Abingdon., 29(9), 887-890. https://doi.org/10.1080/14786419.2014.991929
Mandić BM, Vlajić MD, Trifunović S, Simić M, Vujisić LV, Vucković IM, Novaković M, Nikolić-Mandić SD, Tešević V, Vajs V, Milosavljević S. Optimisation of isolation procedure for pyrrolizidine alkaloids from Rindera umbellata Bunge. in Natural Product Research. 2015;29(9):887-890. doi:10.1080/14786419.2014.991929 .
Mandić, Boris M., Vlajić, Marina D., Trifunović, Snežana, Simić, Milena, Vujisić, Ljubodrag V., Vucković, Ivan M., Novaković, Miroslav, Nikolić-Mandić, Snežana D., Tešević, Vele, Vajs, Vlatka, Milosavljević, Slobodan, "Optimisation of isolation procedure for pyrrolizidine alkaloids from Rindera umbellata Bunge" in Natural Product Research, 29, no. 9 (2015):887-890, https://doi.org/10.1080/14786419.2014.991929 . .