Methicillin-resistant Staphylococcus aureus biofilm formation on dacryocystorhinostomy silicone tubes depends on the genetic lineage
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2015
Authors
Cirković, IvanaKnežević, Miroslav
Božić, Dragana

Rasić, Dejan
Larsen, Anders Rhod

Dukić, Slobodanka
Article (Published version)

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The aim of this study was to investigate biofilm formation on silicone tubes by genetically diverse methicillin-resistant Staphylococcus aureus (MRSA) strains. Capacity of biofilm formation on dacryocystorhinostomy silicone tubes was tested on 30 MRSA strains. Identification and methicillin resistance were confirmed by PCR for nuc and mecA genes. Strains were genotypically characterised (SCCmec, agr and spa typing). Biofilm formation was tested in microtiter plate and on silicone tubes. Tested MRSA strains were classified into SCCmec type I (33.3 %), II (3.3 %), III (20.0 %), IV (26.7 %) and V (16.7 %), agr type I (56.7 %), II (36.7 %) and III (6.6 %), and eight spa clonal complexes (CCs). All tested MRSA strains showed ability to form biofilm on microtiter plate. Capacity of biofilm formation on silicone tubes was as follows: 33.3 % of strains belonged to the category of low biofilm producers, and 66.7 % to moderate biofilm producers. There was statistically significant correlation be...tween spa CC and the category of biofilm production on silicone tubes (p = 0.01): CC5 and CC45 with moderate amount of biofilm, and CC8 with low amount of biofilm. A moderate amount of biofilm formed on silicone tubes correlated with agr type II MRSA strains (p = 0.008). Biofilm formation by MRSA on silicone tubes is highly dependent on genetic characteristics of the strains. Therefore, MRSA genotyping may aid the determination of the possibility of biofilm-related ocular device infections. Genotyping and biofilm quantification may be helpful in determining when decolonisation and cohort isolation are required to prevent device-related infections.
Keywords:
MRSA / Biofilm formation / Silicone tubes / Genetic lineagesSource:
Graefes Archive for Clinical and Experimental Ophthalmology, 2015, 253, 1, 77-82Publisher:
- Springer, New York
Funding / projects:
- Antibiotic resistant bacterial pathogens in Serbia: phenotypic and genotypic characterization (RS-175039)
DOI: 10.1007/s00417-014-2786-0
ISSN: 0721-832X
PubMed: 25209953
WoS: 000347553200009
Scopus: 2-s2.0-84926685836
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Institution/Community
PharmacyTY - JOUR AU - Cirković, Ivana AU - Knežević, Miroslav AU - Božić, Dragana AU - Rasić, Dejan AU - Larsen, Anders Rhod AU - Dukić, Slobodanka PY - 2015 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2414 AB - The aim of this study was to investigate biofilm formation on silicone tubes by genetically diverse methicillin-resistant Staphylococcus aureus (MRSA) strains. Capacity of biofilm formation on dacryocystorhinostomy silicone tubes was tested on 30 MRSA strains. Identification and methicillin resistance were confirmed by PCR for nuc and mecA genes. Strains were genotypically characterised (SCCmec, agr and spa typing). Biofilm formation was tested in microtiter plate and on silicone tubes. Tested MRSA strains were classified into SCCmec type I (33.3 %), II (3.3 %), III (20.0 %), IV (26.7 %) and V (16.7 %), agr type I (56.7 %), II (36.7 %) and III (6.6 %), and eight spa clonal complexes (CCs). All tested MRSA strains showed ability to form biofilm on microtiter plate. Capacity of biofilm formation on silicone tubes was as follows: 33.3 % of strains belonged to the category of low biofilm producers, and 66.7 % to moderate biofilm producers. There was statistically significant correlation between spa CC and the category of biofilm production on silicone tubes (p = 0.01): CC5 and CC45 with moderate amount of biofilm, and CC8 with low amount of biofilm. A moderate amount of biofilm formed on silicone tubes correlated with agr type II MRSA strains (p = 0.008). Biofilm formation by MRSA on silicone tubes is highly dependent on genetic characteristics of the strains. Therefore, MRSA genotyping may aid the determination of the possibility of biofilm-related ocular device infections. Genotyping and biofilm quantification may be helpful in determining when decolonisation and cohort isolation are required to prevent device-related infections. PB - Springer, New York T2 - Graefes Archive for Clinical and Experimental Ophthalmology T1 - Methicillin-resistant Staphylococcus aureus biofilm formation on dacryocystorhinostomy silicone tubes depends on the genetic lineage VL - 253 IS - 1 SP - 77 EP - 82 DO - 10.1007/s00417-014-2786-0 ER -
@article{ author = "Cirković, Ivana and Knežević, Miroslav and Božić, Dragana and Rasić, Dejan and Larsen, Anders Rhod and Dukić, Slobodanka", year = "2015", abstract = "The aim of this study was to investigate biofilm formation on silicone tubes by genetically diverse methicillin-resistant Staphylococcus aureus (MRSA) strains. Capacity of biofilm formation on dacryocystorhinostomy silicone tubes was tested on 30 MRSA strains. Identification and methicillin resistance were confirmed by PCR for nuc and mecA genes. Strains were genotypically characterised (SCCmec, agr and spa typing). Biofilm formation was tested in microtiter plate and on silicone tubes. Tested MRSA strains were classified into SCCmec type I (33.3 %), II (3.3 %), III (20.0 %), IV (26.7 %) and V (16.7 %), agr type I (56.7 %), II (36.7 %) and III (6.6 %), and eight spa clonal complexes (CCs). All tested MRSA strains showed ability to form biofilm on microtiter plate. Capacity of biofilm formation on silicone tubes was as follows: 33.3 % of strains belonged to the category of low biofilm producers, and 66.7 % to moderate biofilm producers. There was statistically significant correlation between spa CC and the category of biofilm production on silicone tubes (p = 0.01): CC5 and CC45 with moderate amount of biofilm, and CC8 with low amount of biofilm. A moderate amount of biofilm formed on silicone tubes correlated with agr type II MRSA strains (p = 0.008). Biofilm formation by MRSA on silicone tubes is highly dependent on genetic characteristics of the strains. Therefore, MRSA genotyping may aid the determination of the possibility of biofilm-related ocular device infections. Genotyping and biofilm quantification may be helpful in determining when decolonisation and cohort isolation are required to prevent device-related infections.", publisher = "Springer, New York", journal = "Graefes Archive for Clinical and Experimental Ophthalmology", title = "Methicillin-resistant Staphylococcus aureus biofilm formation on dacryocystorhinostomy silicone tubes depends on the genetic lineage", volume = "253", number = "1", pages = "77-82", doi = "10.1007/s00417-014-2786-0" }
Cirković, I., Knežević, M., Božić, D., Rasić, D., Larsen, A. R.,& Dukić, S.. (2015). Methicillin-resistant Staphylococcus aureus biofilm formation on dacryocystorhinostomy silicone tubes depends on the genetic lineage. in Graefes Archive for Clinical and Experimental Ophthalmology Springer, New York., 253(1), 77-82. https://doi.org/10.1007/s00417-014-2786-0
Cirković I, Knežević M, Božić D, Rasić D, Larsen AR, Dukić S. Methicillin-resistant Staphylococcus aureus biofilm formation on dacryocystorhinostomy silicone tubes depends on the genetic lineage. in Graefes Archive for Clinical and Experimental Ophthalmology. 2015;253(1):77-82. doi:10.1007/s00417-014-2786-0 .
Cirković, Ivana, Knežević, Miroslav, Božić, Dragana, Rasić, Dejan, Larsen, Anders Rhod, Dukić, Slobodanka, "Methicillin-resistant Staphylococcus aureus biofilm formation on dacryocystorhinostomy silicone tubes depends on the genetic lineage" in Graefes Archive for Clinical and Experimental Ophthalmology, 253, no. 1 (2015):77-82, https://doi.org/10.1007/s00417-014-2786-0 . .