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dc.creatorNovović, Katarina
dc.creatorMihajlović, Sanja
dc.creatorVasiljević, Zorica
dc.creatorFilipić, Brankica
dc.creatorBegović, Jelena
dc.creatorJovčić, Branko
dc.date.accessioned2019-09-02T11:47:32Z
dc.date.available2019-09-02T11:47:32Z
dc.date.issued2015
dc.identifier.issn1932-6203
dc.identifier.urihttps://farfar.pharmacy.bg.ac.rs/handle/123456789/2421
dc.description.abstractCarbapenem-resistant A. baumannii present a significant therapeutic challenge for the treatment of nosocomial infections in many European countries. Although it is known that the gradient of A. baumannii prevalence increases from northern to southern Europe, this study provides the first data from Serbia. Twenty-eight carbapenem-resistant A. baumannii clinical isolates were collected at a Serbian pediatric hospital during a 2-year period. The majority of isolates (67.68%) belonged to the sequence type Group 1, European clonal complex II. All isolates harbored intrinsic OXA-51 and AmpC cephalosporinase. OXA-23 was detected in 16 isolates (57.14%), OXA-24 in 23 isolates (82.14%) and OXA-58 in 11 isolates (39.29%). Six of the isolates (21.43%) harbored all of the analyzed oxacillinases, except OXA-143 and OXA-235 that were not detected in this study. Production of oxacillinases was detected in different pulsotypes indicating the presence of horizontal gene transfer. NDM-1, VIM and IMP were not detected in analyzed clinical A. baumannii isolates. ISAba1 insertion sequence was present upstream of OXA-51 in one isolate, upstream of AmpC in 13 isolates and upstream of OXA-23 in 10 isolates. In silico analysis of carO sequences from analyzed A. baumannii isolates revealed the existence of two out of six highly polymorphic CarO variants. The phylogenetic analysis of CarO protein among Acinetobacter species revised the previous classification CarO variants into three groups based on strong bootstraps scores in the tree analysis. Group I comprises four variants (I-IV) while Groups II and III contain only one variant each. One half of the Serbian clinical isolates belong to Group I variant I, while the other half belongs to Group I variant III.en
dc.publisherPublic Library Science, San Francisco
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173019/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourcePLoS One
dc.titleCarbapenem-Resistant Acinetobacter baumannii from Serbia: Revision of CarO Classificationen
dc.typearticle
dc.rights.licenseBY
dcterms.abstractМихајловић, Сања; Филипић, Бранкица; Васиљевић, Зорица; Јовчић, Бранко; Беговић, Јелена; Нововић, Катарина;
dc.citation.volume10
dc.citation.issue3
dc.citation.other10(3): -
dc.citation.rankM21
dc.identifier.wos000352134700197
dc.identifier.doi10.1371/journal.pone.0122793
dc.identifier.pmid25822626
dc.identifier.scopus2-s2.0-84926430178
dc.identifier.fulltexthttps://farfar.pharmacy.bg.ac.rs//bitstream/id/1112/2419.pdf
dc.identifier.rcubconv_3288
dc.type.versionpublishedVersion


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