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Development of New Liquid Chromatographic Method for Mitotane and Its Metabolites Determination in Human Plasma Employing Design of Experiments Methodology
A simple and reliable new HPLC method with UV detection has been developed and validated for simultaneous determination of mitotane and its two metabolites DDA and DDE. Method development was carried out utilizing systematical approach of the design of experiments (DoE) methodology. For estimation of factors influence on selected chromatographic responses and definition of the optimal chromatographic conditions, Box-Behnken experimental design was applied. The defined optimal separation conditions were: column Restek Ultra Aqua C-18 with pre-column Restek Ultra Aqua C-18 operating at temperature 35 degrees C; mixture of acetonitrile and 0.5% formic acid as mobile phase with 1.2mL min(-1) flow rate and detection at 230nm. As sample preparation method, liquid-liquid extraction was chosen. Method was fully validated and LOQ and LOD were experimentally determined. Finally, method was successfully applied for determination of mitotane and its metabolites in plasma samples of patients with a...drenocortical carcinoma.
TY - JOUR
AU - Jančić-Stojanović, Biljana
AU - Vemić, Sava
AU - Elezović, Valentina
AU - Petrović, Ana
AU - Sinadinović, Zoran
AU - Ivanović, Darko
AU - Damjanović, Svetozar S.
AU - Miljković, Branislava
PY - 2015
UR - http://farfar.pharmacy.bg.ac.rs/handle/123456789/2438
AB - A simple and reliable new HPLC method with UV detection has been developed and validated for simultaneous determination of mitotane and its two metabolites DDA and DDE. Method development was carried out utilizing systematical approach of the design of experiments (DoE) methodology. For estimation of factors influence on selected chromatographic responses and definition of the optimal chromatographic conditions, Box-Behnken experimental design was applied. The defined optimal separation conditions were: column Restek Ultra Aqua C-18 with pre-column Restek Ultra Aqua C-18 operating at temperature 35 degrees C; mixture of acetonitrile and 0.5% formic acid as mobile phase with 1.2mL min(-1) flow rate and detection at 230nm. As sample preparation method, liquid-liquid extraction was chosen. Method was fully validated and LOQ and LOD were experimentally determined. Finally, method was successfully applied for determination of mitotane and its metabolites in plasma samples of patients with adrenocortical carcinoma.
PB - Taylor & Francis Inc, Philadelphia
T2 - Journal of Liquid Chromatography & Related Technologies
T1 - Development of New Liquid Chromatographic Method for Mitotane and Its Metabolites Determination in Human Plasma Employing Design of Experiments Methodology
VL - 38
IS - 14
SP - 1371
EP - 1378
DO - 10.1080/10826076.2015.1057645
ER -
@article{
author = "Jančić-Stojanović, Biljana and Vemić, Sava and Elezović, Valentina and Petrović, Ana and Sinadinović, Zoran and Ivanović, Darko and Damjanović, Svetozar S. and Miljković, Branislava",
year = "2015",
url = "http://farfar.pharmacy.bg.ac.rs/handle/123456789/2438",
abstract = "A simple and reliable new HPLC method with UV detection has been developed and validated for simultaneous determination of mitotane and its two metabolites DDA and DDE. Method development was carried out utilizing systematical approach of the design of experiments (DoE) methodology. For estimation of factors influence on selected chromatographic responses and definition of the optimal chromatographic conditions, Box-Behnken experimental design was applied. The defined optimal separation conditions were: column Restek Ultra Aqua C-18 with pre-column Restek Ultra Aqua C-18 operating at temperature 35 degrees C; mixture of acetonitrile and 0.5% formic acid as mobile phase with 1.2mL min(-1) flow rate and detection at 230nm. As sample preparation method, liquid-liquid extraction was chosen. Method was fully validated and LOQ and LOD were experimentally determined. Finally, method was successfully applied for determination of mitotane and its metabolites in plasma samples of patients with adrenocortical carcinoma.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Journal of Liquid Chromatography & Related Technologies",
title = "Development of New Liquid Chromatographic Method for Mitotane and Its Metabolites Determination in Human Plasma Employing Design of Experiments Methodology",
volume = "38",
number = "14",
pages = "1371-1378",
doi = "10.1080/10826076.2015.1057645"
}
Jančić-Stojanović B, Vemić S, Elezović V, Petrović A, Sinadinović Z, Ivanović D, Damjanović SS, Miljković B. Development of New Liquid Chromatographic Method for Mitotane and Its Metabolites Determination in Human Plasma Employing Design of Experiments Methodology. Journal of Liquid Chromatography & Related Technologies. 2015;38(14):1371-1378
Jančić-Stojanović, B., Vemić, S., Elezović, V., Petrović, A., Sinadinović, Z., Ivanović, D., Damjanović, S. S.,& Miljković, B. (2015). Development of New Liquid Chromatographic Method for Mitotane and Its Metabolites Determination in Human Plasma Employing Design of Experiments Methodology.
Journal of Liquid Chromatography & Related TechnologiesTaylor & Francis Inc, Philadelphia., 38(14), 1371-1378.
https://doi.org/10.1080/10826076.2015.1057645
Jančić-Stojanović Biljana, Vemić Sava, Elezović Valentina, Petrović Ana, Sinadinović Zoran, Ivanović Darko, Damjanović Svetozar S., Miljković Branislava, "Development of New Liquid Chromatographic Method for Mitotane and Its Metabolites Determination in Human Plasma Employing Design of Experiments Methodology" 38, no. 14 (2015):1371-1378,
https://doi.org/10.1080/10826076.2015.1057645 .
