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dc.creatorJančić-Stojanović, Biljana
dc.creatorVemić, Sava
dc.creatorElezović, Valentina
dc.creatorPetrović, Ana
dc.creatorSinadinović, Zoran
dc.creatorIvanović, Darko
dc.creatorDamjanović, Svetozar S.
dc.creatorMiljković, Branislava
dc.date.accessioned2019-09-02T11:48:01Z
dc.date.available2019-09-02T11:48:01Z
dc.date.issued2015
dc.identifier.issn1082-6076
dc.identifier.urihttps://farfar.pharmacy.bg.ac.rs/handle/123456789/2438
dc.description.abstractA simple and reliable new HPLC method with UV detection has been developed and validated for simultaneous determination of mitotane and its two metabolites DDA and DDE. Method development was carried out utilizing systematical approach of the design of experiments (DoE) methodology. For estimation of factors influence on selected chromatographic responses and definition of the optimal chromatographic conditions, Box-Behnken experimental design was applied. The defined optimal separation conditions were: column Restek Ultra Aqua C-18 with pre-column Restek Ultra Aqua C-18 operating at temperature 35 degrees C; mixture of acetonitrile and 0.5% formic acid as mobile phase with 1.2mL min(-1) flow rate and detection at 230nm. As sample preparation method, liquid-liquid extraction was chosen. Method was fully validated and LOQ and LOD were experimentally determined. Finally, method was successfully applied for determination of mitotane and its metabolites in plasma samples of patients with adrenocortical carcinoma.en
dc.publisherTaylor & Francis Inc, Philadelphia
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172052/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Liquid Chromatography & Related Technologies
dc.subjectliquid chromatographyen
dc.subjectmetabolites DDA and DDEen
dc.subjectvalidationen
dc.subjectexperimental designen
dc.subjectmitotaneen
dc.titleDevelopment of New Liquid Chromatographic Method for Mitotane and Its Metabolites Determination in Human Plasma Employing Design of Experiments Methodologyen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractВемић, Сава; Јанчић-Стојановић, Биљана; Дамјановић, Светозар С.; Ивановић, Дарко; Елезовић, Валентина; Петровић, Aна; Синадиновић, Зоран; Миљковић, Бранислава;
dc.citation.volume38
dc.citation.issue14
dc.citation.spage1371
dc.citation.epage1378
dc.citation.other38(14): 1371-1378
dc.citation.rankM23
dc.description.otherPeer-reviewed manuscript:[http://farfar.pharmacy.bg.ac.rs/handle/123456789/3432]
dc.identifier.wos000359492000003
dc.identifier.doi10.1080/10826076.2015.1057645
dc.identifier.scopus2-s2.0-84938869801
dc.identifier.rcubconv_3362
dc.type.versionpublishedVersion


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