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Mechanisms underlying the vasorelaxation of human internal mammary artery induced by (-)-epicatechin
dc.creator | Novaković, Aleksandra | |
dc.creator | Marinko, Marija | |
dc.creator | Vranić, Aleksandra | |
dc.creator | Janković, Goran | |
dc.creator | Milojević, Predrag | |
dc.creator | Stojanović, Ivan | |
dc.creator | Nenezić, Dragoslav | |
dc.creator | Ugrešić, Nenad | |
dc.creator | Kanjuh, Vladimir | |
dc.creator | Yang, Qin | |
dc.creator | He, Guo-Wei | |
dc.date.accessioned | 2019-09-02T11:48:01Z | |
dc.date.available | 2019-09-02T11:48:01Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 0014-2999 | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/2439 | |
dc.description.abstract | Evidences have suggested that flavanol compound (-)-epicatechin is associated with reduced risk of cardiovascular diseases. One of the mechanisms of its cardioprotective effect is vasodilation. However, the exact mechanisms by which (-)-epicatechin causes vasodilation are not yet clearly defined. The aims of the present study were to investigate relaxant effect of flavanol (-)-epicatechin on the isolated human internal mammary artery (HIMA) and to determine the mechanisms underlying its vasorelaxation. Our results showed that (-)-epicatechin induced a concentration-dependent relaxation of RNA rings pre-contracted by phenylephrine. Among the K+ channel blockers, 4-aminopyricline (4-AP) and margatoxin, blockers of voltage gated K+ (K-V) channels, and glibenclamide, a selective ATP sensitive K+ (K-ATP,) channels blocker, partly inhibited the (-)-epicatechin-induced relaxation of HIMA, while iberiotoxin, a most selective blocker of large conductance Ca2+-activated K+ channels (BKCa), almost completely inhibited the relaxation. In rings pre-contracted by 80 mM K+, (-)-epicatechin induced partial relaxation of HIMA, whereas in Ca2+-free medium, (-)-epicatechin completely relaxed HIMA rings pre-contracted by phenylephrine and caffeine. Finally, thapsigargin, a sarcoplasmic reticulum Ca2+-ATPase inhibitor, slightly antagonized (-)-epicatechin-induced relaxation of HIMA pre-contracted by phenylephrine. These results suggest that (-)-epicatechin induces strong endothelium independent relaxation of HIMA pre-contracted by phenylephrine whilst 4-AP- and rnargatoxin-sensitive K-V channels, as well as BKCa and K-ATP channels, located in vascular smooth muscle, mediate this relaxation. In addition, it seems that (-)-epicatechin could inhibit influx of extracellular Ca2+, interfere with intracellular Ca2+ release and re uptake by the sarcoplasmic reticulum. | en |
dc.publisher | Elsevier Science BV, Amsterdam | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175088/RS// | |
dc.rights | restrictedAccess | |
dc.source | European Journal of Pharmacology | |
dc.title | Mechanisms underlying the vasorelaxation of human internal mammary artery induced by (-)-epicatechin | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Маринко, Марија; Yанг, Qин; Кањух, Владимир; Угрешић, Ненад; Хе, Гуо-Wеи; Ненезић, Драгослав; Новаковић, Aлександра; Стојановић, Иван; Милојевић, Предраг; Јанковић, Горан; Вранић, Aлександра; | |
dc.citation.volume | 762 | |
dc.citation.spage | 306 | |
dc.citation.epage | 312 | |
dc.citation.other | 762: 306-312 | |
dc.citation.rank | M22 | |
dc.identifier.wos | 000359711100038 | |
dc.identifier.doi | 10.1016/j.ejphar.2015.05.066 | |
dc.identifier.pmid | 26049011 | |
dc.identifier.scopus | 2-s2.0-84931273854 | |
dc.type.version | publishedVersion |