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Effects of the polyphenol resveratrol on contractility of human term pregnant myometrium
dc.creator | Novaković, Radmila | |
dc.creator | Radunović, Nebojša | |
dc.creator | Marković-Lipkovski, Jasmina | |
dc.creator | Cirović, S. | |
dc.creator | Beleslin-Cokić, Bojana B. | |
dc.creator | Ilić, B. | |
dc.creator | Ivković, Branka | |
dc.creator | Heinle, Helmut | |
dc.creator | Živanović, Vladimir | |
dc.creator | Gojković-Bukarica, Ljiljana | |
dc.date.accessioned | 2019-09-02T11:48:33Z | |
dc.date.available | 2019-09-02T11:48:33Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 1360-9947 | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/2462 | |
dc.description.abstract | The ideal agent for prevention and treatment of uterine abnormal contractility has not been found. The polyphenol resveratrol possesses a wide spectrum of pharmacologic properties, but its influence on the contractility of human myometrium is not defined. The present study evaluated the effect of resveratrol on the oxytocin-induced contractions of human term pregnant myometrium in vitro and the contribution of different K+ channels to resveratrol action. Resveratrol induced a concentration-dependent relaxation of myometrium contractions (pD(2) value and maximal responses were 4.52 and 82.25%, respectively). Glibenclamide, a selective blocker of ATP-sensitive (K-ATP), iberiotoxin, a selective blockers of big-calcium sensitive (BKCa) and 4-aminopiridine, a non-selective blocker of voltage-sensitive (Kv) channels induced a significant shift to the right of the concentration-response curves of resveratrol. Inhibition achieved by 0.1 mM resveratrol was insensitive to all K+ channel blockers. A K+ channel opener, pinacidil, inhibited oxytocin-induced contractions of pregnant myometrium with comparable potency and efficacy to resveratrol (pD(2) values and maximal relaxation were 4.52 and 83.67%, respectively). Based on K+ channel opener/blocker affinities, it appears that the inhibitory response of resveratrol involves different myometrial K+ channels. When applied in high concentrations, resveratrol has an additional K+-channel-independent mechanism(s) of action. Furthermore, immunohistochemistry staining and western blot analyses detected the presence and distribution of K-ATP, BKCa and Kv channel proteins in pregnant myometrium. | en |
dc.publisher | Oxford Univ Press, Oxford | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31020/RS// | |
dc.rights | openAccess | |
dc.source | Molecular Human Reproduction | |
dc.subject | contraction | en |
dc.subject | K+- channels | en |
dc.subject | myometrium | en |
dc.subject | pregnancy | en |
dc.subject | resveratrol | en |
dc.title | Effects of the polyphenol resveratrol on contractility of human term pregnant myometrium | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Ивковић, Бранка; Новаковић, Радмила; Илић, Б.; Цировић, С.; Радуновић, Небојша; Хеинле, Хелмут; Марковић-Липковски, Јасмина; Белеслин-Цокић, Бојана Б.; Живановић, Владимир; Гојковић-Букарица, Љиљана; | |
dc.citation.volume | 21 | |
dc.citation.issue | 6 | |
dc.citation.spage | 545 | |
dc.citation.epage | 551 | |
dc.citation.other | 21(6): 545-551 | |
dc.citation.rank | M21 | |
dc.identifier.wos | 000356261000006 | |
dc.identifier.doi | 10.1093/molehr/gav011 | |
dc.identifier.pmid | 25750187 | |
dc.identifier.scopus | 2-s2.0-84942107911 | |
dc.identifier.fulltext | https://farfar.pharmacy.bg.ac.rs//bitstream/id/1141/2460.pdf | |
dc.type.version | publishedVersion |