Alternations of oxidative stress markers in patients with severe haemophilia treated by different prophylaxis regimens

Đunić, Irena; Dopsaj, Violeta; Miljić, Predrag; Novković, Aleksandra; Suvajdžić-Vuković, Nada; Tomin, Dragica; Elezović, I

doi:10.1111/jth.12993

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2015

Authors

Đunić, Irena
Dopsaj, Violeta

Miljić, Predrag
Novković, Aleksandra
Suvajdžić-Vuković, Nada
Tomin, Dragica
Elezović, I

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Abstract

Background: Due to recurrent bleding in haemophilia patients, the effect of blood, i.e. free iron in the joint spaces, could initiate releasing markers of oxidative stress in physiological fluids. Aims: The aim of this study were to estimate the influence of differ- ent prophylaxis regimens for severe haemophilia on alternations of oxidative stress markers in serum: a) advanced oxidation protein products (AOPP), b) paraoxonase-1 (PON1) and c) sulfhydril groups (SHG). Methods: The study included 20 adult patients with severe haemo- philia. Five patients with haemophilia A received prophylaxis with FVIII concentrate in the standard dose of 20 IU kg 1 three times per week, while another five patients with haemophilia A were given an intermediate dose of FVIII - 10–15 IU kg 1 thrice weekly. Seven patients with haemophilia A and three with haemophilia B, received FVIII/IX concentrate only on-demand. The following oxidative stress markers were measured: a) AOPP, b) PON1 and c)...

Source:
Journal of Thrombosis and Haemostasis, 2015, 13, Supplement 2, 353-353

Publisher:

Elsevier Inc

Note:

XXV Congress of the International Society on Thrombosis and Haemostasis, June 20–25, 2015

DOI: 10.1111/jth.12993

ISSN: 1538-7933

TY - CONF
AU - Đunić, Irena
AU - Dopsaj, Violeta
AU - Miljić, Predrag
AU - Novković, Aleksandra
AU - Suvajdžić-Vuković, Nada
AU - Tomin, Dragica
AU - Elezović, I
PY - 2015
UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2463
AB - Background: Due to recurrent bleding in haemophilia patients, the
effect of blood, i.e. free iron in the joint spaces, could initiate releasing
markers of oxidative stress in physiological fluids.
Aims: The aim of this study were to estimate the influence of differ-
ent prophylaxis regimens for severe haemophilia on alternations of
oxidative stress markers in serum: a) advanced oxidation protein
products (AOPP), b) paraoxonase-1 (PON1) and c) sulfhydril
groups (SHG).
Methods: The study included 20 adult patients with severe haemo-
philia. Five patients with haemophilia A received prophylaxis with
FVIII concentrate in the standard dose of 20 IU kg 1 three times per
week, while another five patients with haemophilia A were given an
intermediate dose of FVIII - 10–15 IU kg 1 thrice weekly. Seven
patients with haemophilia A and three with haemophilia B, received
FVIII/IX concentrate only on-demand. The following oxidative stress
markers were measured: a) AOPP, b) PON1 and c) SHG. Serum sam-
ples were collected initially, before the start of treatment (labelled
AOPP-1, PON1-1 and SHG-1) and after 3 months follow-up (labelled
AOPP-2, PON1-2 and SHG-2).
Results: The mean age of the patients was 32 years (range 19–55).
In the group of patients given standard dose prophylaxis, the mean
values of AOPP-2 (P = 0.018), PON1-2 (P = 0.043) and SHG-2
(P = 0.045) were significantly lower than those for AOPP-1, PON1-
1 and SHG-1. Likewise, the mean values for AOPP-2 (P = 0.047)
and PON1-2 (P = 0.039) in the five patients receiving intermediate
dose prophylaxis were also decreased when compared to initial val-
ues, but SHG level was not significantly changed. In patients trea-
ted on demand the mean values for AOPP, PON1 and SHG did
not alter significantly.
Conclusion: The results of this investigation showed that treatment of
patients with severe haemophilia by continuous prophylaxis with
intravenously applied FVIII/IX provides less oxidative stress reflected
as decreasing of markers such as AOPP, PON1 and SHG.
PB - Elsevier Inc
C3 - Journal of Thrombosis and Haemostasis
T1 - Alternations of oxidative stress markers in patients with severe haemophilia treated by different prophylaxis regimens
VL - 13
IS - Supplement 2
SP - 353
EP - 353
DO - 10.1111/jth.12993
ER -

@conference{
author = "Đunić, Irena and Dopsaj, Violeta and Miljić, Predrag and Novković, Aleksandra and Suvajdžić-Vuković, Nada and Tomin, Dragica and Elezović, I",
year = "2015",
abstract = "Background: Due to recurrent bleding in haemophilia patients, the
effect of blood, i.e. free iron in the joint spaces, could initiate releasing
markers of oxidative stress in physiological fluids.
Aims: The aim of this study were to estimate the influence of differ-
ent prophylaxis regimens for severe haemophilia on alternations of
oxidative stress markers in serum: a) advanced oxidation protein
products (AOPP), b) paraoxonase-1 (PON1) and c) sulfhydril
groups (SHG).
Methods: The study included 20 adult patients with severe haemo-
philia. Five patients with haemophilia A received prophylaxis with
FVIII concentrate in the standard dose of 20 IU kg 1 three times per
week, while another five patients with haemophilia A were given an
intermediate dose of FVIII - 10–15 IU kg 1 thrice weekly. Seven
patients with haemophilia A and three with haemophilia B, received
FVIII/IX concentrate only on-demand. The following oxidative stress
markers were measured: a) AOPP, b) PON1 and c) SHG. Serum sam-
ples were collected initially, before the start of treatment (labelled
AOPP-1, PON1-1 and SHG-1) and after 3 months follow-up (labelled
AOPP-2, PON1-2 and SHG-2).
Results: The mean age of the patients was 32 years (range 19–55).
In the group of patients given standard dose prophylaxis, the mean
values of AOPP-2 (P = 0.018), PON1-2 (P = 0.043) and SHG-2
(P = 0.045) were significantly lower than those for AOPP-1, PON1-
1 and SHG-1. Likewise, the mean values for AOPP-2 (P = 0.047)
and PON1-2 (P = 0.039) in the five patients receiving intermediate
dose prophylaxis were also decreased when compared to initial val-
ues, but SHG level was not significantly changed. In patients trea-
ted on demand the mean values for AOPP, PON1 and SHG did
not alter significantly.
Conclusion: The results of this investigation showed that treatment of
patients with severe haemophilia by continuous prophylaxis with
intravenously applied FVIII/IX provides less oxidative stress reflected
as decreasing of markers such as AOPP, PON1 and SHG.",
publisher = "Elsevier Inc",
journal = "Journal of Thrombosis and Haemostasis",
title = "Alternations of oxidative stress markers in patients with severe haemophilia treated by different prophylaxis regimens",
volume = "13",
number = "Supplement 2",
pages = "353-353",
doi = "10.1111/jth.12993"
}

Đunić, I., Dopsaj, V., Miljić, P., Novković, A., Suvajdžić-Vuković, N., Tomin, D.,& Elezović, I.. (2015). Alternations of oxidative stress markers in patients with severe haemophilia treated by different prophylaxis regimens. in Journal of Thrombosis and Haemostasis
Elsevier Inc., 13(Supplement 2), 353-353.
https://doi.org/10.1111/jth.12993

Đunić I, Dopsaj V, Miljić P, Novković A, Suvajdžić-Vuković N, Tomin D, Elezović I. Alternations of oxidative stress markers in patients with severe haemophilia treated by different prophylaxis regimens. in Journal of Thrombosis and Haemostasis. 2015;13(Supplement 2):353-353.
doi:10.1111/jth.12993 .

Đunić, Irena, Dopsaj, Violeta, Miljić, Predrag, Novković, Aleksandra, Suvajdžić-Vuković, Nada, Tomin, Dragica, Elezović, I, "Alternations of oxidative stress markers in patients with severe haemophilia treated by different prophylaxis regimens" in Journal of Thrombosis and Haemostasis, 13, no. Supplement 2 (2015):353-353,
https://doi.org/10.1111/jth.12993 . .