Fluocortolone and its esters are synthetic corticosteroids used topically in the treatment of various skin disorders. A method that can be successfully used for the separation and determination of fluocortolone pivalate and fluocortolone hexanoate in suppositories was developed. This method is based on reverse-phase HPLC on Supelcosil LC-18 (25 cm x 4.6 mm, 5 mu m), using methanol-acetonitrile-water-glacial acetic acid (17: 46:37:0.4 v/v/v/v) as mobile phase at a flow rate of 3.0 ml/min. Detection was carried out using a UV detector at 238 nm. The method developed was validated, and calibration curves were established dependent on peak area. The validated ranges for fluocortolone pivalate and fluocortolone hexanoate are 15-305 mu g/ml (r = 0.9995) and 15-315 mu g/ml (r = 0.9996), respectively. The limits of detection and the limits of quantification for both esters were also determined.
TY - JOUR
AU - Čudina, Olivera
AU - Brborić, Jasmina
AU - Vujić, Zorica
AU - Radulović, D
AU - Vladimirov, S
PY - 2000
UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/247
AB - Fluocortolone and its esters are synthetic corticosteroids used topically in the treatment of various skin disorders. A method that can be successfully used for the separation and determination of fluocortolone pivalate and fluocortolone hexanoate in suppositories was developed. This method is based on reverse-phase HPLC on Supelcosil LC-18 (25 cm x 4.6 mm, 5 mu m), using methanol-acetonitrile-water-glacial acetic acid (17: 46:37:0.4 v/v/v/v) as mobile phase at a flow rate of 3.0 ml/min. Detection was carried out using a UV detector at 238 nm. The method developed was validated, and calibration curves were established dependent on peak area. The validated ranges for fluocortolone pivalate and fluocortolone hexanoate are 15-305 mu g/ml (r = 0.9995) and 15-315 mu g/ml (r = 0.9996), respectively. The limits of detection and the limits of quantification for both esters were also determined.
PB - Elsevier Science Sa, Lausanne
T2 - Farmaco
T1 - Determination of fluocortolone pivalate and fluocortolone hexanoate in suppositories using reverse-phase HPLC
VL - 55
IS - 2
SP - 125
EP - 127
DO - 10.1016/S0014-827X(00)00003-3
ER -
@article{
author = "Čudina, Olivera and Brborić, Jasmina and Vujić, Zorica and Radulović, D and Vladimirov, S",
year = "2000",
abstract = "Fluocortolone and its esters are synthetic corticosteroids used topically in the treatment of various skin disorders. A method that can be successfully used for the separation and determination of fluocortolone pivalate and fluocortolone hexanoate in suppositories was developed. This method is based on reverse-phase HPLC on Supelcosil LC-18 (25 cm x 4.6 mm, 5 mu m), using methanol-acetonitrile-water-glacial acetic acid (17: 46:37:0.4 v/v/v/v) as mobile phase at a flow rate of 3.0 ml/min. Detection was carried out using a UV detector at 238 nm. The method developed was validated, and calibration curves were established dependent on peak area. The validated ranges for fluocortolone pivalate and fluocortolone hexanoate are 15-305 mu g/ml (r = 0.9995) and 15-315 mu g/ml (r = 0.9996), respectively. The limits of detection and the limits of quantification for both esters were also determined.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Farmaco",
title = "Determination of fluocortolone pivalate and fluocortolone hexanoate in suppositories using reverse-phase HPLC",
volume = "55",
number = "2",
pages = "125-127",
doi = "10.1016/S0014-827X(00)00003-3"
}
Čudina, O., Brborić, J., Vujić, Z., Radulović, D.,& Vladimirov, S.. (2000). Determination of fluocortolone pivalate and fluocortolone hexanoate in suppositories using reverse-phase HPLC. in Farmaco
Elsevier Science Sa, Lausanne., 55(2), 125-127.
https://doi.org/10.1016/S0014-827X(00)00003-3
Čudina O, Brborić J, Vujić Z, Radulović D, Vladimirov S. Determination of fluocortolone pivalate and fluocortolone hexanoate in suppositories using reverse-phase HPLC. in Farmaco. 2000;55(2):125-127.
doi:10.1016/S0014-827X(00)00003-3 .
Čudina, Olivera, Brborić, Jasmina, Vujić, Zorica, Radulović, D, Vladimirov, S, "Determination of fluocortolone pivalate and fluocortolone hexanoate in suppositories using reverse-phase HPLC" in Farmaco, 55, no. 2 (2000):125-127,
https://doi.org/10.1016/S0014-827X(00)00003-3 . .
