Trametes species have been used for thousands of years in traditional and conventional medicine for the treatment of various types of diseases. The goal was to evaluate possible antigenotoxic effects of mycelium and basidiocarp extracts of selected Trametes species and to assess dependence on their antioxidant potential. Trametes versicolor, T. hirsuta, and T. gibbosa were the species studied. Antigenotoxic potentials of extracts were assessed on human peripheral white blood cells with basidiocarp and mycelium extracts of the species. The alkaline comet test was used for detection of DNA strand breaks and alkali-labile sites, as well as the extent of DNA migration. DPPH assay was used to estimate antioxidative properties of extracts. Fruiting body extracts of T. versicolor and T. gibbosa as well as T. hirsuta extracts, except that at 20.0 mg/mL, were not genotoxic agents. T. versicolor extract had at 5.0 mg/mL the greatest antigenotoxic effect in both pre-and posttreatment of leukocyte...s. The mycelium extracts of the three species had no genotoxic activity and significant antigenotoxic effect against H lt inf>2 lt /inf>O lt inf>2 lt /inf>-induced DNA damage, both in pre-and posttreatment. The results suggest that extracts of these three species could be considered as strong antigenotoxic agents able to stimulate genoprotective response of cells.
TY - JOUR
AU - Knežević, A
AU - Živković, Lada
AU - Stajić, Mirjana
AU - Vukojević, Jelena
AU - Milovanović, I
AU - Potparević, Biljana
PY - 2015
UR - http://farfar.pharmacy.bg.ac.rs/handle/123456789/2495
AB - Trametes species have been used for thousands of years in traditional and conventional medicine for the treatment of various types of diseases. The goal was to evaluate possible antigenotoxic effects of mycelium and basidiocarp extracts of selected Trametes species and to assess dependence on their antioxidant potential. Trametes versicolor, T. hirsuta, and T. gibbosa were the species studied. Antigenotoxic potentials of extracts were assessed on human peripheral white blood cells with basidiocarp and mycelium extracts of the species. The alkaline comet test was used for detection of DNA strand breaks and alkali-labile sites, as well as the extent of DNA migration. DPPH assay was used to estimate antioxidative properties of extracts. Fruiting body extracts of T. versicolor and T. gibbosa as well as T. hirsuta extracts, except that at 20.0 mg/mL, were not genotoxic agents. T. versicolor extract had at 5.0 mg/mL the greatest antigenotoxic effect in both pre-and posttreatment of leukocytes. The mycelium extracts of the three species had no genotoxic activity and significant antigenotoxic effect against H lt inf>2 lt /inf>O lt inf>2 lt /inf>-induced DNA damage, both in pre-and posttreatment. The results suggest that extracts of these three species could be considered as strong antigenotoxic agents able to stimulate genoprotective response of cells.
PB - Hindawi Publishing Corporation
T2 - Scientific World Journal
T1 - Antigenotoxic Effect of Trametes spp. Extracts against DNA Damage on Human Peripheral White Blood Cells
VL - 2015
DO - 10.1155/2015/146378
ER -
@article{
author = "Knežević, A and Živković, Lada and Stajić, Mirjana and Vukojević, Jelena and Milovanović, I and Potparević, Biljana",
year = "2015",
url = "http://farfar.pharmacy.bg.ac.rs/handle/123456789/2495",
abstract = "Trametes species have been used for thousands of years in traditional and conventional medicine for the treatment of various types of diseases. The goal was to evaluate possible antigenotoxic effects of mycelium and basidiocarp extracts of selected Trametes species and to assess dependence on their antioxidant potential. Trametes versicolor, T. hirsuta, and T. gibbosa were the species studied. Antigenotoxic potentials of extracts were assessed on human peripheral white blood cells with basidiocarp and mycelium extracts of the species. The alkaline comet test was used for detection of DNA strand breaks and alkali-labile sites, as well as the extent of DNA migration. DPPH assay was used to estimate antioxidative properties of extracts. Fruiting body extracts of T. versicolor and T. gibbosa as well as T. hirsuta extracts, except that at 20.0 mg/mL, were not genotoxic agents. T. versicolor extract had at 5.0 mg/mL the greatest antigenotoxic effect in both pre-and posttreatment of leukocytes. The mycelium extracts of the three species had no genotoxic activity and significant antigenotoxic effect against H lt inf>2 lt /inf>O lt inf>2 lt /inf>-induced DNA damage, both in pre-and posttreatment. The results suggest that extracts of these three species could be considered as strong antigenotoxic agents able to stimulate genoprotective response of cells.",
publisher = "Hindawi Publishing Corporation",
journal = "Scientific World Journal",
title = "Antigenotoxic Effect of Trametes spp. Extracts against DNA Damage on Human Peripheral White Blood Cells",
volume = "2015",
doi = "10.1155/2015/146378"
}
Knežević, A., Živković, L., Stajić, M., Vukojević, J., Milovanović, I.,& Potparević, B. (2015). Antigenotoxic Effect of Trametes spp. Extracts against DNA Damage on Human Peripheral White Blood Cells.
Scientific World Journal
Hindawi Publishing Corporation., 2015.
https://doi.org/10.1155/2015/146378
Knežević A, Živković L, Stajić M, Vukojević J, Milovanović I, Potparević B. Antigenotoxic Effect of Trametes spp. Extracts against DNA Damage on Human Peripheral White Blood Cells. Scientific World Journal. 2015;2015
Knežević A, Živković Lada, Stajić Mirjana, Vukojević Jelena, Milovanović I, Potparević Biljana, "Antigenotoxic Effect of Trametes spp. Extracts against DNA Damage on Human Peripheral White Blood Cells" Scientific World Journal, 2015 (2015),
https://doi.org/10.1155/2015/146378 .
