A method has been developed for the separation of hydrochlorothiazide and amiloride by high-performance liquid chromatographic (HPLC) method on a C-18 column with detection at 280 nm. The optimal conditions of separation were determined with the aid of 'window diagram' technique of Laub and Purnell. The effect of simultaneously varying the pH, proportion aqueous acetic acid and methanol in the mobile phase were studied to optimize the separation.,A response surface diagram was used to optimize the experimental conditions for the separation. The mobile phase composition that provides an acceptable resolution hydrochlorothiazide and amiloride in a short elution time is water:methanol (60:40) and pH 3.2 (pH adjusted to 3.2 with CH3COOH). A method is applied for the quantitative analysis of Moduretic((R)) tablets (Merck Sharp & Dokme International). The powdered tablets are extracted with methanol, containing caffeine as the internal standard; and assayed by comparison of peak areas after ...liquid chromatography.
Source:
Journal of Pharmaceutical and Biomedical Analysis, 2000, 22, 1, 1-6
TY - JOUR
AU - Zečević, Mira
AU - Živanović, LJ
AU - Agatonović-Kuštrin, Snežana
AU - Ivanović, D
AU - Maksimović, M
PY - 2000
UR - http://farfar.pharmacy.bg.ac.rs/handle/123456789/252
AB - A method has been developed for the separation of hydrochlorothiazide and amiloride by high-performance liquid chromatographic (HPLC) method on a C-18 column with detection at 280 nm. The optimal conditions of separation were determined with the aid of 'window diagram' technique of Laub and Purnell. The effect of simultaneously varying the pH, proportion aqueous acetic acid and methanol in the mobile phase were studied to optimize the separation.,A response surface diagram was used to optimize the experimental conditions for the separation. The mobile phase composition that provides an acceptable resolution hydrochlorothiazide and amiloride in a short elution time is water:methanol (60:40) and pH 3.2 (pH adjusted to 3.2 with CH3COOH). A method is applied for the quantitative analysis of Moduretic((R)) tablets (Merck Sharp & Dokme International). The powdered tablets are extracted with methanol, containing caffeine as the internal standard; and assayed by comparison of peak areas after liquid chromatography.
PB - Pergamon-Elsevier Science Ltd, Oxford
T2 - Journal of Pharmaceutical and Biomedical Analysis
T1 - Statistical optimization of a reversed-phase liquid chromatographic method for the analysis of amiloride and hydrochlorothiazide in tablets
VL - 22
IS - 1
SP - 1
EP - 6
DO - 10.1016/S0731-7085(99)00253-8
ER -
@article{
author = "Zečević, Mira and Živanović, LJ and Agatonović-Kuštrin, Snežana and Ivanović, D and Maksimović, M",
year = "2000",
url = "http://farfar.pharmacy.bg.ac.rs/handle/123456789/252",
abstract = "A method has been developed for the separation of hydrochlorothiazide and amiloride by high-performance liquid chromatographic (HPLC) method on a C-18 column with detection at 280 nm. The optimal conditions of separation were determined with the aid of 'window diagram' technique of Laub and Purnell. The effect of simultaneously varying the pH, proportion aqueous acetic acid and methanol in the mobile phase were studied to optimize the separation.,A response surface diagram was used to optimize the experimental conditions for the separation. The mobile phase composition that provides an acceptable resolution hydrochlorothiazide and amiloride in a short elution time is water:methanol (60:40) and pH 3.2 (pH adjusted to 3.2 with CH3COOH). A method is applied for the quantitative analysis of Moduretic((R)) tablets (Merck Sharp & Dokme International). The powdered tablets are extracted with methanol, containing caffeine as the internal standard; and assayed by comparison of peak areas after liquid chromatography.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Statistical optimization of a reversed-phase liquid chromatographic method for the analysis of amiloride and hydrochlorothiazide in tablets",
volume = "22",
number = "1",
pages = "1-6",
doi = "10.1016/S0731-7085(99)00253-8"
}
Zečević, M., Živanović, L., Agatonović-Kuštrin, S., Ivanović, D.,& Maksimović, M. (2000). Statistical optimization of a reversed-phase liquid chromatographic method for the analysis of amiloride and hydrochlorothiazide in tablets.
Journal of Pharmaceutical and Biomedical Analysis
Pergamon-Elsevier Science Ltd, Oxford., 22(1), 1-6.
https://doi.org/10.1016/S0731-7085(99)00253-8
Zečević M, Živanović L, Agatonović-Kuštrin S, Ivanović D, Maksimović M. Statistical optimization of a reversed-phase liquid chromatographic method for the analysis of amiloride and hydrochlorothiazide in tablets. Journal of Pharmaceutical and Biomedical Analysis. 2000;22(1):1-6
Zečević Mira, Živanović LJ, Agatonović-Kuštrin Snežana, Ivanović D, Maksimović M, "Statistical optimization of a reversed-phase liquid chromatographic method for the analysis of amiloride and hydrochlorothiazide in tablets" Journal of Pharmaceutical and Biomedical Analysis, 22, no. 1 (2000):1-6,
https://doi.org/10.1016/S0731-7085(99)00253-8 .
