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dc.creatorHou, Hai-Tao
dc.creatorWang, Jun
dc.creatorWang, Zheng-Qing
dc.creatorLiu, Xiao-Cheng
dc.creatorMarinko, Marija
dc.creatorNovaković, Aleksandra
dc.creatorYang, Qin
dc.creatorHe, Guo-Wei
dc.date.accessioned2019-09-02T11:50:19Z
dc.date.available2019-09-02T11:50:19Z
dc.date.issued2016
dc.identifier.issn0003-4975
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/2525
dc.description.abstractBackground. Graft spasm remains challenging in coronary artery bypass grafting (CABG). Calcium antagonists are commonly used in patients with coronary artery disease. This study investigated the inhibitory effect of third-generation dihydropyridine calcium channel antagonist benidipine on the vasoconstriction induced by various vasoconstrictors in the human internalmammary artery(IMA). Methods. Isolated human IMA rings (N = 65, taken from 37 patients undergoing CABG) were studied in a myograph in 2 ways: the relaxing effect of benidipine on vasoconstrictor-induced precontraction by KCl and U46619 and the depressing effect of benidipine at plasma concentrations on the contraction. Enzyme-linked immunosorbent assay (ELISA) was used to measure the change of the protein related to the L-type calcium channel. Results. Benidipine caused more relaxation in KCl-contracted (86.7% +/- 3.3%; n = 12) than in U46619-contracted (63.8% +/- 5.3%; n = 8; p lt 0.001) IMA rings. Pretreatment of IMA with plasma concentrations of benidipine (-6.92 log M) significantly depressed subsequent contraction by KCl (from 17.3 +/- 2.7 mN to 7.4 +/- 1.2 mN; n = 6; p lt 0.05) but did not significantly affect the contraction caused by U46619. Benidipine also caused a decrease of caveolin (CaV) 1.2 protein content (0.55 +/- 0.02 versus 0.63 +/- 0.02 mg/mL; p lt 0.05). Conclusions. We conclude that in human IMA, the third-generation dihydropyridine calcium channel antagonist benidipine has a potent inhibitory effect on the vasoconstriction mediated by a variety of vasoconstrictors. Use of benidipine in patients undergoing CABG may provide vasorelaxant or antispastic effects in the grafts.en
dc.publisherElsevier Science Inc, New York
dc.relationTianjin Binhai Key Platform for Creative Research Program - 2012-BH110004
dc.relationBinhai New Area Health Bureau - 2012BWKZ008
dc.relationTianjin Health Bureau - 2013KZ009
dc.relationNational Science and Technology Major Project - 2013ZX09303004-005
dc.relationHangzhou Science and Technology Projects - 20140633B12
dc.relationBinhai New Area Health Bureau - 2014BWKY002
dc.relationBinhai New Area Health Bureau - 2014BWKY010
dc.relationTianjin Health Bureau - 2014KZ005
dc.relationNational Natural Science Foundation of China - 81170148
dc.relationZhejiang Provincial Natural Science Foundation - LY15H020008
dc.rightsrestrictedAccess
dc.sourceAnnals of Thoracic Surgery
dc.titleEffect of Benidipine in Human Internal Mammary Artery and Clinical Implicationsen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractМаринко, Марија; Хоу, Хаи-Тао; Wанг, Зхенг-Qинг; Wанг, Јун; Новаковић, Aлександра; Yанг, Qин; Лиу, Xиао-Цхенг; Хе, Гуо-Wеи;
dc.citation.volume101
dc.citation.issue5
dc.citation.spage1789
dc.citation.epage1795
dc.citation.other101(5): 1789-1795
dc.citation.rankM21
dc.identifier.wos000375868500040
dc.identifier.doi10.1016/j.athoracsur.2015.10.029
dc.identifier.pmid26707005
dc.identifier.scopus2-s2.0-84963976346
dc.identifier.rcubconv_3574
dc.type.versionpublishedVersion


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