Chemical Composition and Antimicrobial Activity of the Essential Oil and Methanol Extract of Hypericum aegypticum subsp. webbii (Spach) N. Robson
Abstract
Hypericum aegypticum subsp. webbii is an evergreen shrub spread in Mediterranean part of central and southeastern Europe. The chemical composition and antimicrobial activity of the essential oil and MeOH extract of H. aegypticum subsp. webbii were investigated. The monoterpenes alpha-pinene (63.4 - 68.5%) and beta-pinene (16.9 - 17.0%) were main compounds in the volatile oil from aerial parts. In the cluster analysis, the essential oil of H. aegypticum subsp. webbii was separated and chemically different from the oil of other subspecies of H. aegypticum as well as other Hypericum species from Greece. SIMPER analysis revealed that alpha-pinene (24.79%) was the component that contributed the most to differences between all oils. Also, there was extremely high overall dissimilarity between three subspecies of H. aegypticum. MeOH extract of aerial parts of H. aegypticum subsp. webbii contained flavonoids rutin (56.4 +/- 0.9 mg/g), hyperoside and quercetin, and phenolic acids chlorogenic an...d caffeic acid, while naphthodianthrones were not detected. The antimicrobial activity of essential oil was moderate (MIC from 100 to >200 mu g/ml), while MeOH extract inhibited the growth of Gram-positive bacteria Bacillus subtilis, Enterococcus faecalis, Staphylococcus epidermidis, and Micrococcus luteus (MIC 50 - 100 mu g/ml), more pronounced than the extract of H. perforatum (MIC 200 - >200 mu g/ml).
Source:
Chemistry & Biodiversity, 2016, 13, 4, 427-436Publisher:
- Wiley-VCH Verlag GMBH, Weinheim
Funding / projects:
- Investigation on the medicinal plants: morphological, chemical and pharmacological characterisation (RS-173021)
- Plant Biodiversity of Serbia and the Balkans - assesment, sustainable use and protection (RS-173030)
DOI: 10.1002/cbdv.201500119
ISSN: 1612-1872
PubMed: 26923468
WoS: 000378005200009
Scopus: 2-s2.0-84964342072
Collections
Institution/Community
PharmacyTY - JOUR AU - Marčetić, Mirjana AU - Milenković, Marina AU - Lakušić, Dmitar AU - Lakušić, Branislava PY - 2016 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2533 AB - Hypericum aegypticum subsp. webbii is an evergreen shrub spread in Mediterranean part of central and southeastern Europe. The chemical composition and antimicrobial activity of the essential oil and MeOH extract of H. aegypticum subsp. webbii were investigated. The monoterpenes alpha-pinene (63.4 - 68.5%) and beta-pinene (16.9 - 17.0%) were main compounds in the volatile oil from aerial parts. In the cluster analysis, the essential oil of H. aegypticum subsp. webbii was separated and chemically different from the oil of other subspecies of H. aegypticum as well as other Hypericum species from Greece. SIMPER analysis revealed that alpha-pinene (24.79%) was the component that contributed the most to differences between all oils. Also, there was extremely high overall dissimilarity between three subspecies of H. aegypticum. MeOH extract of aerial parts of H. aegypticum subsp. webbii contained flavonoids rutin (56.4 +/- 0.9 mg/g), hyperoside and quercetin, and phenolic acids chlorogenic and caffeic acid, while naphthodianthrones were not detected. The antimicrobial activity of essential oil was moderate (MIC from 100 to >200 mu g/ml), while MeOH extract inhibited the growth of Gram-positive bacteria Bacillus subtilis, Enterococcus faecalis, Staphylococcus epidermidis, and Micrococcus luteus (MIC 50 - 100 mu g/ml), more pronounced than the extract of H. perforatum (MIC 200 - >200 mu g/ml). PB - Wiley-VCH Verlag GMBH, Weinheim T2 - Chemistry & Biodiversity T1 - Chemical Composition and Antimicrobial Activity of the Essential Oil and Methanol Extract of Hypericum aegypticum subsp. webbii (Spach) N. Robson VL - 13 IS - 4 SP - 427 EP - 436 DO - 10.1002/cbdv.201500119 ER -
@article{ author = "Marčetić, Mirjana and Milenković, Marina and Lakušić, Dmitar and Lakušić, Branislava", year = "2016", abstract = "Hypericum aegypticum subsp. webbii is an evergreen shrub spread in Mediterranean part of central and southeastern Europe. The chemical composition and antimicrobial activity of the essential oil and MeOH extract of H. aegypticum subsp. webbii were investigated. The monoterpenes alpha-pinene (63.4 - 68.5%) and beta-pinene (16.9 - 17.0%) were main compounds in the volatile oil from aerial parts. In the cluster analysis, the essential oil of H. aegypticum subsp. webbii was separated and chemically different from the oil of other subspecies of H. aegypticum as well as other Hypericum species from Greece. SIMPER analysis revealed that alpha-pinene (24.79%) was the component that contributed the most to differences between all oils. Also, there was extremely high overall dissimilarity between three subspecies of H. aegypticum. MeOH extract of aerial parts of H. aegypticum subsp. webbii contained flavonoids rutin (56.4 +/- 0.9 mg/g), hyperoside and quercetin, and phenolic acids chlorogenic and caffeic acid, while naphthodianthrones were not detected. The antimicrobial activity of essential oil was moderate (MIC from 100 to >200 mu g/ml), while MeOH extract inhibited the growth of Gram-positive bacteria Bacillus subtilis, Enterococcus faecalis, Staphylococcus epidermidis, and Micrococcus luteus (MIC 50 - 100 mu g/ml), more pronounced than the extract of H. perforatum (MIC 200 - >200 mu g/ml).", publisher = "Wiley-VCH Verlag GMBH, Weinheim", journal = "Chemistry & Biodiversity", title = "Chemical Composition and Antimicrobial Activity of the Essential Oil and Methanol Extract of Hypericum aegypticum subsp. webbii (Spach) N. Robson", volume = "13", number = "4", pages = "427-436", doi = "10.1002/cbdv.201500119" }
Marčetić, M., Milenković, M., Lakušić, D.,& Lakušić, B.. (2016). Chemical Composition and Antimicrobial Activity of the Essential Oil and Methanol Extract of Hypericum aegypticum subsp. webbii (Spach) N. Robson. in Chemistry & Biodiversity Wiley-VCH Verlag GMBH, Weinheim., 13(4), 427-436. https://doi.org/10.1002/cbdv.201500119
Marčetić M, Milenković M, Lakušić D, Lakušić B. Chemical Composition and Antimicrobial Activity of the Essential Oil and Methanol Extract of Hypericum aegypticum subsp. webbii (Spach) N. Robson. in Chemistry & Biodiversity. 2016;13(4):427-436. doi:10.1002/cbdv.201500119 .
Marčetić, Mirjana, Milenković, Marina, Lakušić, Dmitar, Lakušić, Branislava, "Chemical Composition and Antimicrobial Activity of the Essential Oil and Methanol Extract of Hypericum aegypticum subsp. webbii (Spach) N. Robson" in Chemistry & Biodiversity, 13, no. 4 (2016):427-436, https://doi.org/10.1002/cbdv.201500119 . .