Biofilm formation on tympanostomy tubes depends on methicillin-resistant Staphylococcus aureus genetic lineage
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2016
Authors
Jotić, AnaBožić, Dragana

Milovanović, Jovica
Pavlović, Bojan
Jesić, Snežana
Pelemis, Mijomir
Novaković, Marko
Cirković, Ivana
Article (Published version)

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Bacterial biofilm formation has been implicated in the high incidence of persistent otorrhoea after tympanostomy tube insertion. The aim of the study was to investigate whether biofilm formation on tympanostomy tubes depends on the genetic profile of methicillin-resistant Staphylococcus aureus (MRSA) strains. Capacity of biofilm formation on fluoroplastic tympanostomy tubes (TTs) was tested on 30 MRSA strains. Identification and methicillin resistance were confirmed by PCR for nuc and mecA genes. Strains were genotypically characterised (SCCmec, agr and spa typing). Biofilm formation was tested in microtiter plate and on TTs. Tested MRSA strains were classified into SCCmec type I (36.7 %), III (23.3 %), IV (26.7 %) and V (13.3 %), agr type I (50 %), II (36.7 %) and III (13.3 %), and 5 clonal complexes (CCs). All tested MRSA strains showed ability to form biofilm on microtiter plate. Capacity of biofilm formation on TTs was as following: 13.3 % of strains belonged to the category of no ...biofilm producers, 50 % to the category of weak biofilm producers and 36.7 % to moderate biofilm producers. There was a statistically significant difference between CC, SCCmec and agr types and the category of biofilm production on TTs tubes (p lt 0.001): CC5, SCCmecI type and agrII type with a moderate amount of biofilm, and CC8 and agrI type with a low amount of biofilm. Biofilm formation by MRSA on TTs is highly dependent on genetic characteristics of the strains. Therefore, MRSA genotyping may aid the determination of the possibility of biofilm-related post-tympanostomy tube otorrhea.
Source:
European Archives of Oto-Rhino-Laryngology, 2016, 273, 3, 615-620Publisher:
- Springer, New York
Funding / projects:
- Antibiotic resistant bacterial pathogens in Serbia: phenotypic and genotypic characterization (RS-175039)
DOI: 10.1007/s00405-015-3607-8
ISSN: 0937-4477
PubMed: 25796207
WoS: 000371063700013
Scopus: 2-s2.0-84959138728
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PharmacyTY - JOUR AU - Jotić, Ana AU - Božić, Dragana AU - Milovanović, Jovica AU - Pavlović, Bojan AU - Jesić, Snežana AU - Pelemis, Mijomir AU - Novaković, Marko AU - Cirković, Ivana PY - 2016 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2561 AB - Bacterial biofilm formation has been implicated in the high incidence of persistent otorrhoea after tympanostomy tube insertion. The aim of the study was to investigate whether biofilm formation on tympanostomy tubes depends on the genetic profile of methicillin-resistant Staphylococcus aureus (MRSA) strains. Capacity of biofilm formation on fluoroplastic tympanostomy tubes (TTs) was tested on 30 MRSA strains. Identification and methicillin resistance were confirmed by PCR for nuc and mecA genes. Strains were genotypically characterised (SCCmec, agr and spa typing). Biofilm formation was tested in microtiter plate and on TTs. Tested MRSA strains were classified into SCCmec type I (36.7 %), III (23.3 %), IV (26.7 %) and V (13.3 %), agr type I (50 %), II (36.7 %) and III (13.3 %), and 5 clonal complexes (CCs). All tested MRSA strains showed ability to form biofilm on microtiter plate. Capacity of biofilm formation on TTs was as following: 13.3 % of strains belonged to the category of no biofilm producers, 50 % to the category of weak biofilm producers and 36.7 % to moderate biofilm producers. There was a statistically significant difference between CC, SCCmec and agr types and the category of biofilm production on TTs tubes (p lt 0.001): CC5, SCCmecI type and agrII type with a moderate amount of biofilm, and CC8 and agrI type with a low amount of biofilm. Biofilm formation by MRSA on TTs is highly dependent on genetic characteristics of the strains. Therefore, MRSA genotyping may aid the determination of the possibility of biofilm-related post-tympanostomy tube otorrhea. PB - Springer, New York T2 - European Archives of Oto-Rhino-Laryngology T1 - Biofilm formation on tympanostomy tubes depends on methicillin-resistant Staphylococcus aureus genetic lineage VL - 273 IS - 3 SP - 615 EP - 620 DO - 10.1007/s00405-015-3607-8 ER -
@article{ author = "Jotić, Ana and Božić, Dragana and Milovanović, Jovica and Pavlović, Bojan and Jesić, Snežana and Pelemis, Mijomir and Novaković, Marko and Cirković, Ivana", year = "2016", abstract = "Bacterial biofilm formation has been implicated in the high incidence of persistent otorrhoea after tympanostomy tube insertion. The aim of the study was to investigate whether biofilm formation on tympanostomy tubes depends on the genetic profile of methicillin-resistant Staphylococcus aureus (MRSA) strains. Capacity of biofilm formation on fluoroplastic tympanostomy tubes (TTs) was tested on 30 MRSA strains. Identification and methicillin resistance were confirmed by PCR for nuc and mecA genes. Strains were genotypically characterised (SCCmec, agr and spa typing). Biofilm formation was tested in microtiter plate and on TTs. Tested MRSA strains were classified into SCCmec type I (36.7 %), III (23.3 %), IV (26.7 %) and V (13.3 %), agr type I (50 %), II (36.7 %) and III (13.3 %), and 5 clonal complexes (CCs). All tested MRSA strains showed ability to form biofilm on microtiter plate. Capacity of biofilm formation on TTs was as following: 13.3 % of strains belonged to the category of no biofilm producers, 50 % to the category of weak biofilm producers and 36.7 % to moderate biofilm producers. There was a statistically significant difference between CC, SCCmec and agr types and the category of biofilm production on TTs tubes (p lt 0.001): CC5, SCCmecI type and agrII type with a moderate amount of biofilm, and CC8 and agrI type with a low amount of biofilm. Biofilm formation by MRSA on TTs is highly dependent on genetic characteristics of the strains. Therefore, MRSA genotyping may aid the determination of the possibility of biofilm-related post-tympanostomy tube otorrhea.", publisher = "Springer, New York", journal = "European Archives of Oto-Rhino-Laryngology", title = "Biofilm formation on tympanostomy tubes depends on methicillin-resistant Staphylococcus aureus genetic lineage", volume = "273", number = "3", pages = "615-620", doi = "10.1007/s00405-015-3607-8" }
Jotić, A., Božić, D., Milovanović, J., Pavlović, B., Jesić, S., Pelemis, M., Novaković, M.,& Cirković, I.. (2016). Biofilm formation on tympanostomy tubes depends on methicillin-resistant Staphylococcus aureus genetic lineage. in European Archives of Oto-Rhino-Laryngology Springer, New York., 273(3), 615-620. https://doi.org/10.1007/s00405-015-3607-8
Jotić A, Božić D, Milovanović J, Pavlović B, Jesić S, Pelemis M, Novaković M, Cirković I. Biofilm formation on tympanostomy tubes depends on methicillin-resistant Staphylococcus aureus genetic lineage. in European Archives of Oto-Rhino-Laryngology. 2016;273(3):615-620. doi:10.1007/s00405-015-3607-8 .
Jotić, Ana, Božić, Dragana, Milovanović, Jovica, Pavlović, Bojan, Jesić, Snežana, Pelemis, Mijomir, Novaković, Marko, Cirković, Ivana, "Biofilm formation on tympanostomy tubes depends on methicillin-resistant Staphylococcus aureus genetic lineage" in European Archives of Oto-Rhino-Laryngology, 273, no. 3 (2016):615-620, https://doi.org/10.1007/s00405-015-3607-8 . .