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dc.creatorJotić, Ana
dc.creatorBožić, Dragana
dc.creatorMilovanović, Jovica
dc.creatorPavlović, Bojan
dc.creatorJesić, Snežana
dc.creatorPelemis, Mijomir
dc.creatorNovaković, Marko
dc.creatorCirković, Ivana
dc.date.accessioned2019-09-02T11:51:14Z
dc.date.available2019-09-02T11:51:14Z
dc.date.issued2016
dc.identifier.issn0937-4477
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/2561
dc.description.abstractBacterial biofilm formation has been implicated in the high incidence of persistent otorrhoea after tympanostomy tube insertion. The aim of the study was to investigate whether biofilm formation on tympanostomy tubes depends on the genetic profile of methicillin-resistant Staphylococcus aureus (MRSA) strains. Capacity of biofilm formation on fluoroplastic tympanostomy tubes (TTs) was tested on 30 MRSA strains. Identification and methicillin resistance were confirmed by PCR for nuc and mecA genes. Strains were genotypically characterised (SCCmec, agr and spa typing). Biofilm formation was tested in microtiter plate and on TTs. Tested MRSA strains were classified into SCCmec type I (36.7 %), III (23.3 %), IV (26.7 %) and V (13.3 %), agr type I (50 %), II (36.7 %) and III (13.3 %), and 5 clonal complexes (CCs). All tested MRSA strains showed ability to form biofilm on microtiter plate. Capacity of biofilm formation on TTs was as following: 13.3 % of strains belonged to the category of no biofilm producers, 50 % to the category of weak biofilm producers and 36.7 % to moderate biofilm producers. There was a statistically significant difference between CC, SCCmec and agr types and the category of biofilm production on TTs tubes (p lt 0.001): CC5, SCCmecI type and agrII type with a moderate amount of biofilm, and CC8 and agrI type with a low amount of biofilm. Biofilm formation by MRSA on TTs is highly dependent on genetic characteristics of the strains. Therefore, MRSA genotyping may aid the determination of the possibility of biofilm-related post-tympanostomy tube otorrhea.en
dc.publisherSpringer, New York
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175039/RS//
dc.rightsrestrictedAccess
dc.sourceEuropean Archives of Oto-Rhino-Laryngology
dc.titleBiofilm formation on tympanostomy tubes depends on methicillin-resistant Staphylococcus aureus genetic lineageen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractПавловић, Бојан; Јотић, Aна; Божић, Драгана; Миловановић, Јовица; Јесић, Снежана; Пелемис, Мијомир; Новаковић, Марко; Цирковић, Ивана;
dc.citation.volume273
dc.citation.issue3
dc.citation.spage615
dc.citation.epage620
dc.citation.other273(3): 615-620
dc.citation.rankM22
dc.identifier.wos000371063700013
dc.identifier.doi10.1007/s00405-015-3607-8
dc.identifier.pmid25796207
dc.identifier.scopus2-s2.0-84959138728
dc.identifier.rcubconv_3506
dc.type.versionpublishedVersion


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