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dc.creatorFoivas, Anargyros
dc.creatorMalenović, Anđelija
dc.creatorKostić, Nada
dc.creatorBožić, Marija
dc.creatorKnežević, Miroslav
dc.creatorLoukas, Yannis L.
dc.creatorDotsikas, Yannis
dc.date.accessioned2019-09-02T11:51:23Z
dc.date.available2019-09-02T11:51:23Z
dc.date.issued2016
dc.identifier.issn0731-7085
dc.identifier.urihttps://farfar.pharmacy.bg.ac.rs/handle/123456789/2566
dc.description.abstractIn the current study, a rapid and sensitive LC-QTOF-MS/MS method for the determination of brinzolamide in dried blood spots (DBS) was developed and validated. This novel sample collection, storage and transfer technique was suitable for analyzing a drug with high distribution into red blood cells and negligible plasma levels. The method included an isocratic mobile phase consisting of methanol and 10 mM ammonium formate (90:10, v/v) and detection in positive electrospray mode (ESI+). The flow rate was adjusted to 0.350 mL/min yielding retention times of 1.7 min for both brinzolamide and internal standard (IS) rabeprazole on a Cyano analytical column, respectively. The validation of the proposed method over the concentration range 0.500-20.0 mu g/mL was performed in compliance with EMEA and FDA guidelines, assessing all major performance characteristics. Inter- and intra- assay precisions were less than 14%, while inter- and intra- assay accuracies varied from 922 to 111%. No matrix effect was observed and the mean brinzolamide extraction recovery was 93.5%. The method was successfully applied to real DBS samples from patients in steady state condition, receiving brinzolamide ophthalmic suspension 1% (w/v) for several months. Initial concentrations were corrected due to hematocrit effect, using image processing algorithm written in Matlab.en
dc.publisherElsevier Science BV, Amsterdam
dc.rightsrestrictedAccess
dc.sourceJournal of Pharmaceutical and Biomedical Analysis
dc.titleQuantitation of brinzolamide in dried blood spots by a novel LC-QTOF-MS/MS methoden
dc.typearticle
dc.rights.licenseARR
dcterms.abstractМаленовић, Aнђелија; Дотсикас, Yаннис; Лоукас, Yаннис Л.; Костић, Нада; Кнежевић, Мирослав; Божић, Марија; Фоивас, Aнаргyрос;
dc.citation.volume119
dc.citation.spage84
dc.citation.epage90
dc.citation.other119: 84-90
dc.citation.rankM21
dc.identifier.wos000370211900012
dc.identifier.doi10.1016/j.jpba.2015.11.043
dc.identifier.pmid26669612
dc.identifier.scopus2-s2.0-84949255283
dc.identifier.rcubconv_3488
dc.type.versionpublishedVersion


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