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Quantitation of brinzolamide in dried blood spots by a novel LC-QTOF-MS/MS method
dc.creator | Foivas, Anargyros | |
dc.creator | Malenović, Anđelija | |
dc.creator | Kostić, Nada | |
dc.creator | Božić, Marija | |
dc.creator | Knežević, Miroslav | |
dc.creator | Loukas, Yannis L. | |
dc.creator | Dotsikas, Yannis | |
dc.date.accessioned | 2019-09-02T11:51:23Z | |
dc.date.available | 2019-09-02T11:51:23Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 0731-7085 | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/2566 | |
dc.description.abstract | In the current study, a rapid and sensitive LC-QTOF-MS/MS method for the determination of brinzolamide in dried blood spots (DBS) was developed and validated. This novel sample collection, storage and transfer technique was suitable for analyzing a drug with high distribution into red blood cells and negligible plasma levels. The method included an isocratic mobile phase consisting of methanol and 10 mM ammonium formate (90:10, v/v) and detection in positive electrospray mode (ESI+). The flow rate was adjusted to 0.350 mL/min yielding retention times of 1.7 min for both brinzolamide and internal standard (IS) rabeprazole on a Cyano analytical column, respectively. The validation of the proposed method over the concentration range 0.500-20.0 mu g/mL was performed in compliance with EMEA and FDA guidelines, assessing all major performance characteristics. Inter- and intra- assay precisions were less than 14%, while inter- and intra- assay accuracies varied from 922 to 111%. No matrix effect was observed and the mean brinzolamide extraction recovery was 93.5%. The method was successfully applied to real DBS samples from patients in steady state condition, receiving brinzolamide ophthalmic suspension 1% (w/v) for several months. Initial concentrations were corrected due to hematocrit effect, using image processing algorithm written in Matlab. | en |
dc.publisher | Elsevier Science BV, Amsterdam | |
dc.rights | restrictedAccess | |
dc.source | Journal of Pharmaceutical and Biomedical Analysis | |
dc.title | Quantitation of brinzolamide in dried blood spots by a novel LC-QTOF-MS/MS method | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Маленовић, Aнђелија; Дотсикас, Yаннис; Лоукас, Yаннис Л.; Костић, Нада; Кнежевић, Мирослав; Божић, Марија; Фоивас, Aнаргyрос; | |
dc.citation.volume | 119 | |
dc.citation.spage | 84 | |
dc.citation.epage | 90 | |
dc.citation.other | 119: 84-90 | |
dc.citation.rank | M21 | |
dc.identifier.wos | 000370211900012 | |
dc.identifier.doi | 10.1016/j.jpba.2015.11.043 | |
dc.identifier.pmid | 26669612 | |
dc.identifier.scopus | 2-s2.0-84949255283 | |
dc.type.version | publishedVersion |