Chemical Composition and Bioactivity of the Essential Oils of Heracleum pyrenaicum subsp. pollinianum and Heracleum orphanidis
Autori
Ušjak, LjubošPetrović, Silvana
Drobac, Milica
Soković, Marina
Stanojković, Tatjana
Ćirić, Ana D.
Niketić, Marjan
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The objective of this research was to analyze the chemical composition, antimicrobial and cytotoxic activity of Heracleum pyrenaicum subsp. pollinianum
(Bertol.) F. Pedrotti & Pignatti (HPP) and H. orphanidis Boiss. (HO) essential oils. The composition of the oils was analyzed by GC and GC-MS. β-Pinene
(35.1%) was the most abundant compound in HPP root oil, while (Z)-falcarinol (80.0%) dominated in HO root oil. (E)-Nerolidol (28.5%) was the main
constituent in HPP leaf oil. HPP fruit oil, as well as HO leaf and fruit oils mainly contained aliphatic esters, mostly octyl acetate (50.5-84.5%). Antimicrobial
screening was performed by microdilution method against eight bacterial and eight fungal strains. The strongest antibacterial activity was shown by both root
oils (MICs 0.02-0.60 mg/mL and MBCs 0.04-2.50 mg/mL for HPP, and MICs 0.02-1.25 mg/mL and MBCs 0.04-2.50 mg/mL for HO), while the best
antifungal potential was exhibited by HPP fruit oil (MICs 0.30-0.60 mg/mL and MFCs 0.60-1....25 mg/mL) and HO leaf oil (MICs 0.15-0.63 mg/mL and MFCs
0.30-1.25 mg/mL). The tested root and fruit oils exhibited strong cytotoxic effect, which was determined by MTT test against HeLa (IC50 7.53-21.07 μg/mL)
and LS174 (IC50 24.16-58.86 μg/mL) cell lines.
Ključne reči:
Antimicrobial activity / Chemical composition / Cytotoxic effect / Essential oils / Heracleum orphanidis / Heracleum pyrenaicum subsp. pollinianumIzvor:
Natural Product Communications, 2016, 11, 4, 529-534Izdavač:
- Natural Products Inc, Westerville
Finansiranje / projekti:
- Ispitivanje lekovitog potencijala biljaka: morfološka, hemijska i farmakološka karakterizacija (RS-MESTD-Basic Research (BR or ON)-173021)
- Karakterizacija i primena metabolita gljiva i utvrđivanje potencijala novih biofungicida (RS-MESTD-Basic Research (BR or ON)-173032)
- Modifikatori biološkog odgovora u fiziološkim i patološkim stanjima (RS-MESTD-Basic Research (BR or ON)-175011)
Institucija/grupa
PharmacyTY - JOUR AU - Ušjak, Ljuboš AU - Petrović, Silvana AU - Drobac, Milica AU - Soković, Marina AU - Stanojković, Tatjana AU - Ćirić, Ana D. AU - Niketić, Marjan PY - 2016 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2571 AB - The objective of this research was to analyze the chemical composition, antimicrobial and cytotoxic activity of Heracleum pyrenaicum subsp. pollinianum (Bertol.) F. Pedrotti & Pignatti (HPP) and H. orphanidis Boiss. (HO) essential oils. The composition of the oils was analyzed by GC and GC-MS. β-Pinene (35.1%) was the most abundant compound in HPP root oil, while (Z)-falcarinol (80.0%) dominated in HO root oil. (E)-Nerolidol (28.5%) was the main constituent in HPP leaf oil. HPP fruit oil, as well as HO leaf and fruit oils mainly contained aliphatic esters, mostly octyl acetate (50.5-84.5%). Antimicrobial screening was performed by microdilution method against eight bacterial and eight fungal strains. The strongest antibacterial activity was shown by both root oils (MICs 0.02-0.60 mg/mL and MBCs 0.04-2.50 mg/mL for HPP, and MICs 0.02-1.25 mg/mL and MBCs 0.04-2.50 mg/mL for HO), while the best antifungal potential was exhibited by HPP fruit oil (MICs 0.30-0.60 mg/mL and MFCs 0.60-1.25 mg/mL) and HO leaf oil (MICs 0.15-0.63 mg/mL and MFCs 0.30-1.25 mg/mL). The tested root and fruit oils exhibited strong cytotoxic effect, which was determined by MTT test against HeLa (IC50 7.53-21.07 μg/mL) and LS174 (IC50 24.16-58.86 μg/mL) cell lines. PB - Natural Products Inc, Westerville T2 - Natural Product Communications T1 - Chemical Composition and Bioactivity of the Essential Oils of Heracleum pyrenaicum subsp. pollinianum and Heracleum orphanidis VL - 11 IS - 4 SP - 529 EP - 534 UR - https://hdl.handle.net/21.15107/rcub_ibiss_2990 ER -
@article{ author = "Ušjak, Ljuboš and Petrović, Silvana and Drobac, Milica and Soković, Marina and Stanojković, Tatjana and Ćirić, Ana D. and Niketić, Marjan", year = "2016", abstract = "The objective of this research was to analyze the chemical composition, antimicrobial and cytotoxic activity of Heracleum pyrenaicum subsp. pollinianum (Bertol.) F. Pedrotti & Pignatti (HPP) and H. orphanidis Boiss. (HO) essential oils. The composition of the oils was analyzed by GC and GC-MS. β-Pinene (35.1%) was the most abundant compound in HPP root oil, while (Z)-falcarinol (80.0%) dominated in HO root oil. (E)-Nerolidol (28.5%) was the main constituent in HPP leaf oil. HPP fruit oil, as well as HO leaf and fruit oils mainly contained aliphatic esters, mostly octyl acetate (50.5-84.5%). Antimicrobial screening was performed by microdilution method against eight bacterial and eight fungal strains. The strongest antibacterial activity was shown by both root oils (MICs 0.02-0.60 mg/mL and MBCs 0.04-2.50 mg/mL for HPP, and MICs 0.02-1.25 mg/mL and MBCs 0.04-2.50 mg/mL for HO), while the best antifungal potential was exhibited by HPP fruit oil (MICs 0.30-0.60 mg/mL and MFCs 0.60-1.25 mg/mL) and HO leaf oil (MICs 0.15-0.63 mg/mL and MFCs 0.30-1.25 mg/mL). The tested root and fruit oils exhibited strong cytotoxic effect, which was determined by MTT test against HeLa (IC50 7.53-21.07 μg/mL) and LS174 (IC50 24.16-58.86 μg/mL) cell lines.", publisher = "Natural Products Inc, Westerville", journal = "Natural Product Communications", title = "Chemical Composition and Bioactivity of the Essential Oils of Heracleum pyrenaicum subsp. pollinianum and Heracleum orphanidis", volume = "11", number = "4", pages = "529-534", url = "https://hdl.handle.net/21.15107/rcub_ibiss_2990" }
Ušjak, L., Petrović, S., Drobac, M., Soković, M., Stanojković, T., Ćirić, A. D.,& Niketić, M.. (2016). Chemical Composition and Bioactivity of the Essential Oils of Heracleum pyrenaicum subsp. pollinianum and Heracleum orphanidis. in Natural Product Communications Natural Products Inc, Westerville., 11(4), 529-534. https://hdl.handle.net/21.15107/rcub_ibiss_2990
Ušjak L, Petrović S, Drobac M, Soković M, Stanojković T, Ćirić AD, Niketić M. Chemical Composition and Bioactivity of the Essential Oils of Heracleum pyrenaicum subsp. pollinianum and Heracleum orphanidis. in Natural Product Communications. 2016;11(4):529-534. https://hdl.handle.net/21.15107/rcub_ibiss_2990 .
Ušjak, Ljuboš, Petrović, Silvana, Drobac, Milica, Soković, Marina, Stanojković, Tatjana, Ćirić, Ana D., Niketić, Marjan, "Chemical Composition and Bioactivity of the Essential Oils of Heracleum pyrenaicum subsp. pollinianum and Heracleum orphanidis" in Natural Product Communications, 11, no. 4 (2016):529-534, https://hdl.handle.net/21.15107/rcub_ibiss_2990 .