Higher circulating resistin protein and PBMCs resistin mRNA levels are associated with increased prevalence of small dense LDL particles in coronary artery disease patients
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2016
Authors
Joksić, JelenaSopić, Miron

Spasojević-Kalimanovska, Vesna

Gojković, Tamara

Zeljković, Aleksandra

Vekić, Jelena

Anđelković, Kristina
Kalimanovska-Oštrić, Dimitra
Jelić-Ivanović, Zorana

Article (Published version)

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Recent in vitro experiments have indicated that human resistin increases the number of lipoprotein particles secreted by the human hepatocytes and also influences their quality, in terms of generating more proatherogenic lipid particles. The aim of this study is to investigate associations of plasma resistin and peripheral blood mononuclear cells (PBMCs) resistin messenger RNA (mRNA) levels with different prevalence of small, dense low-density lipoprotein particles (sdLDL) in patients with indications for coronary angiography. This study included 65 patients requiring coronary angiography. There were 41 patients without significant stenosis and 24 patients with significant stenosis in at least one major coronary artery. Circulating resistin was measured by enzyme-linked immunosorbent assay; PBMC resistin mRNA was determined by real-time polymerase chain reaction. The LDL and high density lipoprotein subclasses were determined by gradient gel electrophoresis. Plasma resistin (P = 0.031)... and PBMCs resistin mRNA (P = 0.004) were significantly higher in patients with proportion of sdLDL particles >= 50%, compared to the group with relative proportion of sdLDL particles lt 50%. Plasma resistin correlated positively with creatinine (r = 0.456, P lt 0.001) and resistin mRNA (r = 0.298, P = 0.014) but negatively with body mass index (r = -0.254, P = 0.034) and total cholesterol (r = -0.286, P = 0.021). Multiple linear regression analysis revealed LDL particle diameter as the only independent predictor of resistin mRNA (R-2 = 0.258; adjR(2) = 0.190). A significant association between resistin, both PBMCs mRNA and plasma protein, and the relative proportion of sdLDL particles in the circulation of coronary artery disease patients has been established, which implies that increased gene expression of resistin in PBMCs and higher resistin concentration in plasma are related to pro-atherogenic LDL particle phenotype.
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Clinical and Experimental Pharmacology and Physiology, 2016, 43, 1, 22-28Publisher:
- Wiley, Hoboken
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DOI: 10.1111/1440-1681.12503
ISSN: 0305-1870
PubMed: 26466550
WoS: 000368345800003
Scopus: 2-s2.0-84954427340
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PharmacyTY - JOUR AU - Joksić, Jelena AU - Sopić, Miron AU - Spasojević-Kalimanovska, Vesna AU - Gojković, Tamara AU - Zeljković, Aleksandra AU - Vekić, Jelena AU - Anđelković, Kristina AU - Kalimanovska-Oštrić, Dimitra AU - Jelić-Ivanović, Zorana PY - 2016 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2575 AB - Recent in vitro experiments have indicated that human resistin increases the number of lipoprotein particles secreted by the human hepatocytes and also influences their quality, in terms of generating more proatherogenic lipid particles. The aim of this study is to investigate associations of plasma resistin and peripheral blood mononuclear cells (PBMCs) resistin messenger RNA (mRNA) levels with different prevalence of small, dense low-density lipoprotein particles (sdLDL) in patients with indications for coronary angiography. This study included 65 patients requiring coronary angiography. There were 41 patients without significant stenosis and 24 patients with significant stenosis in at least one major coronary artery. Circulating resistin was measured by enzyme-linked immunosorbent assay; PBMC resistin mRNA was determined by real-time polymerase chain reaction. The LDL and high density lipoprotein subclasses were determined by gradient gel electrophoresis. Plasma resistin (P = 0.031) and PBMCs resistin mRNA (P = 0.004) were significantly higher in patients with proportion of sdLDL particles >= 50%, compared to the group with relative proportion of sdLDL particles lt 50%. Plasma resistin correlated positively with creatinine (r = 0.456, P lt 0.001) and resistin mRNA (r = 0.298, P = 0.014) but negatively with body mass index (r = -0.254, P = 0.034) and total cholesterol (r = -0.286, P = 0.021). Multiple linear regression analysis revealed LDL particle diameter as the only independent predictor of resistin mRNA (R-2 = 0.258; adjR(2) = 0.190). A significant association between resistin, both PBMCs mRNA and plasma protein, and the relative proportion of sdLDL particles in the circulation of coronary artery disease patients has been established, which implies that increased gene expression of resistin in PBMCs and higher resistin concentration in plasma are related to pro-atherogenic LDL particle phenotype. PB - Wiley, Hoboken T2 - Clinical and Experimental Pharmacology and Physiology T1 - Higher circulating resistin protein and PBMCs resistin mRNA levels are associated with increased prevalence of small dense LDL particles in coronary artery disease patients VL - 43 IS - 1 SP - 22 EP - 28 DO - 10.1111/1440-1681.12503 ER -
@article{ author = "Joksić, Jelena and Sopić, Miron and Spasojević-Kalimanovska, Vesna and Gojković, Tamara and Zeljković, Aleksandra and Vekić, Jelena and Anđelković, Kristina and Kalimanovska-Oštrić, Dimitra and Jelić-Ivanović, Zorana", year = "2016", abstract = "Recent in vitro experiments have indicated that human resistin increases the number of lipoprotein particles secreted by the human hepatocytes and also influences their quality, in terms of generating more proatherogenic lipid particles. The aim of this study is to investigate associations of plasma resistin and peripheral blood mononuclear cells (PBMCs) resistin messenger RNA (mRNA) levels with different prevalence of small, dense low-density lipoprotein particles (sdLDL) in patients with indications for coronary angiography. This study included 65 patients requiring coronary angiography. There were 41 patients without significant stenosis and 24 patients with significant stenosis in at least one major coronary artery. Circulating resistin was measured by enzyme-linked immunosorbent assay; PBMC resistin mRNA was determined by real-time polymerase chain reaction. The LDL and high density lipoprotein subclasses were determined by gradient gel electrophoresis. Plasma resistin (P = 0.031) and PBMCs resistin mRNA (P = 0.004) were significantly higher in patients with proportion of sdLDL particles >= 50%, compared to the group with relative proportion of sdLDL particles lt 50%. Plasma resistin correlated positively with creatinine (r = 0.456, P lt 0.001) and resistin mRNA (r = 0.298, P = 0.014) but negatively with body mass index (r = -0.254, P = 0.034) and total cholesterol (r = -0.286, P = 0.021). Multiple linear regression analysis revealed LDL particle diameter as the only independent predictor of resistin mRNA (R-2 = 0.258; adjR(2) = 0.190). A significant association between resistin, both PBMCs mRNA and plasma protein, and the relative proportion of sdLDL particles in the circulation of coronary artery disease patients has been established, which implies that increased gene expression of resistin in PBMCs and higher resistin concentration in plasma are related to pro-atherogenic LDL particle phenotype.", publisher = "Wiley, Hoboken", journal = "Clinical and Experimental Pharmacology and Physiology", title = "Higher circulating resistin protein and PBMCs resistin mRNA levels are associated with increased prevalence of small dense LDL particles in coronary artery disease patients", volume = "43", number = "1", pages = "22-28", doi = "10.1111/1440-1681.12503" }
Joksić, J., Sopić, M., Spasojević-Kalimanovska, V., Gojković, T., Zeljković, A., Vekić, J., Anđelković, K., Kalimanovska-Oštrić, D.,& Jelić-Ivanović, Z.. (2016). Higher circulating resistin protein and PBMCs resistin mRNA levels are associated with increased prevalence of small dense LDL particles in coronary artery disease patients. in Clinical and Experimental Pharmacology and Physiology Wiley, Hoboken., 43(1), 22-28. https://doi.org/10.1111/1440-1681.12503
Joksić J, Sopić M, Spasojević-Kalimanovska V, Gojković T, Zeljković A, Vekić J, Anđelković K, Kalimanovska-Oštrić D, Jelić-Ivanović Z. Higher circulating resistin protein and PBMCs resistin mRNA levels are associated with increased prevalence of small dense LDL particles in coronary artery disease patients. in Clinical and Experimental Pharmacology and Physiology. 2016;43(1):22-28. doi:10.1111/1440-1681.12503 .
Joksić, Jelena, Sopić, Miron, Spasojević-Kalimanovska, Vesna, Gojković, Tamara, Zeljković, Aleksandra, Vekić, Jelena, Anđelković, Kristina, Kalimanovska-Oštrić, Dimitra, Jelić-Ivanović, Zorana, "Higher circulating resistin protein and PBMCs resistin mRNA levels are associated with increased prevalence of small dense LDL particles in coronary artery disease patients" in Clinical and Experimental Pharmacology and Physiology, 43, no. 1 (2016):22-28, https://doi.org/10.1111/1440-1681.12503 . .