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Higher circulating resistin protein and PBMCs resistin mRNA levels are associated with increased prevalence of small dense LDL particles in coronary artery disease patients

Authorized Users Only
2016
Authors
Joksić, Jelena
Sopić, Miron
Spasojević-Kalimanovska, Vesna
Gojković, Tamara
Zeljković, Aleksandra
Vekić, Jelena
Anđelković, Kristina
Kalimanovska-Oštrić, Dimitra
Jelić-Ivanović, Zorana
Article (Published version)
Metadata
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Abstract
Recent in vitro experiments have indicated that human resistin increases the number of lipoprotein particles secreted by the human hepatocytes and also influences their quality, in terms of generating more proatherogenic lipid particles. The aim of this study is to investigate associations of plasma resistin and peripheral blood mononuclear cells (PBMCs) resistin messenger RNA (mRNA) levels with different prevalence of small, dense low-density lipoprotein particles (sdLDL) in patients with indications for coronary angiography. This study included 65 patients requiring coronary angiography. There were 41 patients without significant stenosis and 24 patients with significant stenosis in at least one major coronary artery. Circulating resistin was measured by enzyme-linked immunosorbent assay; PBMC resistin mRNA was determined by real-time polymerase chain reaction. The LDL and high density lipoprotein subclasses were determined by gradient gel electrophoresis. Plasma resistin (P = 0.031)... and PBMCs resistin mRNA (P = 0.004) were significantly higher in patients with proportion of sdLDL particles >= 50%, compared to the group with relative proportion of sdLDL particles lt 50%. Plasma resistin correlated positively with creatinine (r = 0.456, P lt 0.001) and resistin mRNA (r = 0.298, P = 0.014) but negatively with body mass index (r = -0.254, P = 0.034) and total cholesterol (r = -0.286, P = 0.021). Multiple linear regression analysis revealed LDL particle diameter as the only independent predictor of resistin mRNA (R-2 = 0.258; adjR(2) = 0.190). A significant association between resistin, both PBMCs mRNA and plasma protein, and the relative proportion of sdLDL particles in the circulation of coronary artery disease patients has been established, which implies that increased gene expression of resistin in PBMCs and higher resistin concentration in plasma are related to pro-atherogenic LDL particle phenotype.

Source:
Clinical and Experimental Pharmacology and Physiology, 2016, 43, 1, 22-28
Publisher:
  • Wiley, Hoboken
Funding / projects:
  • Interactive role of dyslipidemia, oxidative stress and inflammation in atherosclerosis and other diseases: genetic and biochemical markers (RS-175035)

DOI: 10.1111/1440-1681.12503

ISSN: 0305-1870

PubMed: 26466550

WoS: 000368345800003

Scopus: 2-s2.0-84954427340
[ Google Scholar ]
6
5
URI
https://farfar.pharmacy.bg.ac.rs/handle/123456789/2575
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Pharmacy
TY  - JOUR
AU  - Joksić, Jelena
AU  - Sopić, Miron
AU  - Spasojević-Kalimanovska, Vesna
AU  - Gojković, Tamara
AU  - Zeljković, Aleksandra
AU  - Vekić, Jelena
AU  - Anđelković, Kristina
AU  - Kalimanovska-Oštrić, Dimitra
AU  - Jelić-Ivanović, Zorana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2575
AB  - Recent in vitro experiments have indicated that human resistin increases the number of lipoprotein particles secreted by the human hepatocytes and also influences their quality, in terms of generating more proatherogenic lipid particles. The aim of this study is to investigate associations of plasma resistin and peripheral blood mononuclear cells (PBMCs) resistin messenger RNA (mRNA) levels with different prevalence of small, dense low-density lipoprotein particles (sdLDL) in patients with indications for coronary angiography. This study included 65 patients requiring coronary angiography. There were 41 patients without significant stenosis and 24 patients with significant stenosis in at least one major coronary artery. Circulating resistin was measured by enzyme-linked immunosorbent assay; PBMC resistin mRNA was determined by real-time polymerase chain reaction. The LDL and high density lipoprotein subclasses were determined by gradient gel electrophoresis. Plasma resistin (P = 0.031) and PBMCs resistin mRNA (P = 0.004) were significantly higher in patients with proportion of sdLDL particles >= 50%, compared to the group with relative proportion of sdLDL particles  lt  50%. Plasma resistin correlated positively with creatinine (r = 0.456, P  lt  0.001) and resistin mRNA (r = 0.298, P = 0.014) but negatively with body mass index (r = -0.254, P = 0.034) and total cholesterol (r = -0.286, P = 0.021). Multiple linear regression analysis revealed LDL particle diameter as the only independent predictor of resistin mRNA (R-2 = 0.258; adjR(2) = 0.190). A significant association between resistin, both PBMCs mRNA and plasma protein, and the relative proportion of sdLDL particles in the circulation of coronary artery disease patients has been established, which implies that increased gene expression of resistin in PBMCs and higher resistin concentration in plasma are related to pro-atherogenic LDL particle phenotype.
PB  - Wiley, Hoboken
T2  - Clinical and Experimental Pharmacology and Physiology
T1  - Higher circulating resistin protein and PBMCs resistin mRNA levels are associated with increased prevalence of small dense LDL particles in coronary artery disease patients
VL  - 43
IS  - 1
SP  - 22
EP  - 28
DO  - 10.1111/1440-1681.12503
ER  - 
@article{
author = "Joksić, Jelena and Sopić, Miron and Spasojević-Kalimanovska, Vesna and Gojković, Tamara and Zeljković, Aleksandra and Vekić, Jelena and Anđelković, Kristina and Kalimanovska-Oštrić, Dimitra and Jelić-Ivanović, Zorana",
year = "2016",
abstract = "Recent in vitro experiments have indicated that human resistin increases the number of lipoprotein particles secreted by the human hepatocytes and also influences their quality, in terms of generating more proatherogenic lipid particles. The aim of this study is to investigate associations of plasma resistin and peripheral blood mononuclear cells (PBMCs) resistin messenger RNA (mRNA) levels with different prevalence of small, dense low-density lipoprotein particles (sdLDL) in patients with indications for coronary angiography. This study included 65 patients requiring coronary angiography. There were 41 patients without significant stenosis and 24 patients with significant stenosis in at least one major coronary artery. Circulating resistin was measured by enzyme-linked immunosorbent assay; PBMC resistin mRNA was determined by real-time polymerase chain reaction. The LDL and high density lipoprotein subclasses were determined by gradient gel electrophoresis. Plasma resistin (P = 0.031) and PBMCs resistin mRNA (P = 0.004) were significantly higher in patients with proportion of sdLDL particles >= 50%, compared to the group with relative proportion of sdLDL particles  lt  50%. Plasma resistin correlated positively with creatinine (r = 0.456, P  lt  0.001) and resistin mRNA (r = 0.298, P = 0.014) but negatively with body mass index (r = -0.254, P = 0.034) and total cholesterol (r = -0.286, P = 0.021). Multiple linear regression analysis revealed LDL particle diameter as the only independent predictor of resistin mRNA (R-2 = 0.258; adjR(2) = 0.190). A significant association between resistin, both PBMCs mRNA and plasma protein, and the relative proportion of sdLDL particles in the circulation of coronary artery disease patients has been established, which implies that increased gene expression of resistin in PBMCs and higher resistin concentration in plasma are related to pro-atherogenic LDL particle phenotype.",
publisher = "Wiley, Hoboken",
journal = "Clinical and Experimental Pharmacology and Physiology",
title = "Higher circulating resistin protein and PBMCs resistin mRNA levels are associated with increased prevalence of small dense LDL particles in coronary artery disease patients",
volume = "43",
number = "1",
pages = "22-28",
doi = "10.1111/1440-1681.12503"
}
Joksić, J., Sopić, M., Spasojević-Kalimanovska, V., Gojković, T., Zeljković, A., Vekić, J., Anđelković, K., Kalimanovska-Oštrić, D.,& Jelić-Ivanović, Z.. (2016). Higher circulating resistin protein and PBMCs resistin mRNA levels are associated with increased prevalence of small dense LDL particles in coronary artery disease patients. in Clinical and Experimental Pharmacology and Physiology
Wiley, Hoboken., 43(1), 22-28.
https://doi.org/10.1111/1440-1681.12503
Joksić J, Sopić M, Spasojević-Kalimanovska V, Gojković T, Zeljković A, Vekić J, Anđelković K, Kalimanovska-Oštrić D, Jelić-Ivanović Z. Higher circulating resistin protein and PBMCs resistin mRNA levels are associated with increased prevalence of small dense LDL particles in coronary artery disease patients. in Clinical and Experimental Pharmacology and Physiology. 2016;43(1):22-28.
doi:10.1111/1440-1681.12503 .
Joksić, Jelena, Sopić, Miron, Spasojević-Kalimanovska, Vesna, Gojković, Tamara, Zeljković, Aleksandra, Vekić, Jelena, Anđelković, Kristina, Kalimanovska-Oštrić, Dimitra, Jelić-Ivanović, Zorana, "Higher circulating resistin protein and PBMCs resistin mRNA levels are associated with increased prevalence of small dense LDL particles in coronary artery disease patients" in Clinical and Experimental Pharmacology and Physiology, 43, no. 1 (2016):22-28,
https://doi.org/10.1111/1440-1681.12503 . .

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