There is a worldwide ongoing investigation for novel natural constituents with cytotoxic and antioxidant properties. The aim of this study was to investigate chemical profile and stated biological activities of the supercritical CO2 extract (SCE) of old man's beard compared to the extracts obtained using the conventional techniques (Soxhlet extracts and macerate). The most abundant compound identified was usnic acid, which content was inversely proportional to the polarity of the solvent used and was the highest in the SCE, which was the sample revealing the highest cytotoxic activity in tested tumor cell lines (B16 mouse melanoma and C6 rat glioma), with lower IC50 values compared to pure usnic acid. Further investigations suggested both SCE and usnic acid to induce apoptosis and/ or autophagy in B16 and C6, indicating higher cytotoxicity of SCE to be related to the higher degree of ROS production. A good correlation of usnic acid content in the extracts and their antioxidant capacity... was established, extricating SCE as the most active one. Presented results support further investigations of SCE of old man's beard as a prospective therapeutic agent with potential relevance in the treatment of cancer and/ or in oxidative stress-mediated conditions.
TY - JOUR
AU - Žugić, Ana R.
AU - Jeremić, Ivica
AU - Isaković, Aleksandra
AU - Arsić, Ivana A.
AU - Savić, Snežana
AU - Tadić, Vanja M.
PY - 2016
UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2577
AB - There is a worldwide ongoing investigation for novel natural constituents with cytotoxic and antioxidant properties. The aim of this study was to investigate chemical profile and stated biological activities of the supercritical CO2 extract (SCE) of old man's beard compared to the extracts obtained using the conventional techniques (Soxhlet extracts and macerate). The most abundant compound identified was usnic acid, which content was inversely proportional to the polarity of the solvent used and was the highest in the SCE, which was the sample revealing the highest cytotoxic activity in tested tumor cell lines (B16 mouse melanoma and C6 rat glioma), with lower IC50 values compared to pure usnic acid. Further investigations suggested both SCE and usnic acid to induce apoptosis and/ or autophagy in B16 and C6, indicating higher cytotoxicity of SCE to be related to the higher degree of ROS production. A good correlation of usnic acid content in the extracts and their antioxidant capacity was established, extricating SCE as the most active one. Presented results support further investigations of SCE of old man's beard as a prospective therapeutic agent with potential relevance in the treatment of cancer and/ or in oxidative stress-mediated conditions.
PB - Public Library Science, San Francisco
T2 - PLoS One
T1 - Evaluation of Anticancer and Antioxidant Activity of a Commercially Available CO2 Supercritical Extract of Old Man's Beard (Usnea barbata)
VL - 11
IS - 1
DO - 10.1371/journal.pone.0146342
ER -
@article{
author = "Žugić, Ana R. and Jeremić, Ivica and Isaković, Aleksandra and Arsić, Ivana A. and Savić, Snežana and Tadić, Vanja M.",
year = "2016",
abstract = "There is a worldwide ongoing investigation for novel natural constituents with cytotoxic and antioxidant properties. The aim of this study was to investigate chemical profile and stated biological activities of the supercritical CO2 extract (SCE) of old man's beard compared to the extracts obtained using the conventional techniques (Soxhlet extracts and macerate). The most abundant compound identified was usnic acid, which content was inversely proportional to the polarity of the solvent used and was the highest in the SCE, which was the sample revealing the highest cytotoxic activity in tested tumor cell lines (B16 mouse melanoma and C6 rat glioma), with lower IC50 values compared to pure usnic acid. Further investigations suggested both SCE and usnic acid to induce apoptosis and/ or autophagy in B16 and C6, indicating higher cytotoxicity of SCE to be related to the higher degree of ROS production. A good correlation of usnic acid content in the extracts and their antioxidant capacity was established, extricating SCE as the most active one. Presented results support further investigations of SCE of old man's beard as a prospective therapeutic agent with potential relevance in the treatment of cancer and/ or in oxidative stress-mediated conditions.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Evaluation of Anticancer and Antioxidant Activity of a Commercially Available CO2 Supercritical Extract of Old Man's Beard (Usnea barbata)",
volume = "11",
number = "1",
doi = "10.1371/journal.pone.0146342"
}
Žugić, A. R., Jeremić, I., Isaković, A., Arsić, I. A., Savić, S.,& Tadić, V. M.. (2016). Evaluation of Anticancer and Antioxidant Activity of a Commercially Available CO2 Supercritical Extract of Old Man's Beard (Usnea barbata). in PLoS One
Public Library Science, San Francisco., 11(1).
https://doi.org/10.1371/journal.pone.0146342
Žugić AR, Jeremić I, Isaković A, Arsić IA, Savić S, Tadić VM. Evaluation of Anticancer and Antioxidant Activity of a Commercially Available CO2 Supercritical Extract of Old Man's Beard (Usnea barbata). in PLoS One. 2016;11(1).
doi:10.1371/journal.pone.0146342 .
Žugić, Ana R., Jeremić, Ivica, Isaković, Aleksandra, Arsić, Ivana A., Savić, Snežana, Tadić, Vanja M., "Evaluation of Anticancer and Antioxidant Activity of a Commercially Available CO2 Supercritical Extract of Old Man's Beard (Usnea barbata)" in PLoS One, 11, no. 1 (2016),
https://doi.org/10.1371/journal.pone.0146342 . .
