Methylmalonic acid and neutrophil morphometric index in laboratory diagnosis of cobalamin deficiency without macrocytosis
Abstract
IntroductionThis study aimed to investigate the utility of methylmalonic acid (MMA) and neutrophil Cell Population Data, available on the Beckman Coulter LH750 Analyser (Miami, FL, USA), in laboratory assessment of cobalamin status in patients at risk of cobalamin deficiency, without macrocytosis and inflammation. MethodsThe study group included 189 patients. Neutrophil Cell Population Data along with vitamin B12 and homocysteine were assessed in regard to MMA tertile groups. ResultsStatistically significant differences were between lower and upper MMA tertile in serum B12 (P ? 0.001), homocysteine (P = 0.001) and neutrophil morphometric index, NeS-DW (P = 0.029). Also, serum B12 concentrations were significantly different between lower and middle MMA tertile, P = 0.005. Receiver operating characteristic analysis of NeS-DW ability to detect MMA?367 nmol/L revealed a significant area under the curve AUC = 0.761 P ? 0.001 95% CI 0.693-0.830. Optimal cut-off value was NeS-DW?3.51% with se...nsitivity of 74.19% and specificity of 68.87%. ConclusionIn patients at risk of cobalamin deficiency and normal MCV, classification according to MMA revealed cobalamin status differences. Neutrophil morphometric index may be an indicator of early changes in neutrophil nucleus morphology caused by impaired cobalamin status.
Source:
International Journal of Laboratory Hematology, 2016, 38, 3, 265-272Publisher:
- Wiley-Blackwell, Hoboken
Funding / projects:
DOI: 10.1111/ijlh.12479
ISSN: 1751-5521
PubMed: 26992463
WoS: 000375048900012
Scopus: 2-s2.0-84962512577
Collections
Institution/Community
PharmacyTY - JOUR AU - Totosković, Dragana AU - Dopsaj, Violeta AU - Martinović, Jelena PY - 2016 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2589 AB - IntroductionThis study aimed to investigate the utility of methylmalonic acid (MMA) and neutrophil Cell Population Data, available on the Beckman Coulter LH750 Analyser (Miami, FL, USA), in laboratory assessment of cobalamin status in patients at risk of cobalamin deficiency, without macrocytosis and inflammation. MethodsThe study group included 189 patients. Neutrophil Cell Population Data along with vitamin B12 and homocysteine were assessed in regard to MMA tertile groups. ResultsStatistically significant differences were between lower and upper MMA tertile in serum B12 (P ? 0.001), homocysteine (P = 0.001) and neutrophil morphometric index, NeS-DW (P = 0.029). Also, serum B12 concentrations were significantly different between lower and middle MMA tertile, P = 0.005. Receiver operating characteristic analysis of NeS-DW ability to detect MMA?367 nmol/L revealed a significant area under the curve AUC = 0.761 P ? 0.001 95% CI 0.693-0.830. Optimal cut-off value was NeS-DW?3.51% with sensitivity of 74.19% and specificity of 68.87%. ConclusionIn patients at risk of cobalamin deficiency and normal MCV, classification according to MMA revealed cobalamin status differences. Neutrophil morphometric index may be an indicator of early changes in neutrophil nucleus morphology caused by impaired cobalamin status. PB - Wiley-Blackwell, Hoboken T2 - International Journal of Laboratory Hematology T1 - Methylmalonic acid and neutrophil morphometric index in laboratory diagnosis of cobalamin deficiency without macrocytosis VL - 38 IS - 3 SP - 265 EP - 272 DO - 10.1111/ijlh.12479 ER -
@article{ author = "Totosković, Dragana and Dopsaj, Violeta and Martinović, Jelena", year = "2016", abstract = "IntroductionThis study aimed to investigate the utility of methylmalonic acid (MMA) and neutrophil Cell Population Data, available on the Beckman Coulter LH750 Analyser (Miami, FL, USA), in laboratory assessment of cobalamin status in patients at risk of cobalamin deficiency, without macrocytosis and inflammation. MethodsThe study group included 189 patients. Neutrophil Cell Population Data along with vitamin B12 and homocysteine were assessed in regard to MMA tertile groups. ResultsStatistically significant differences were between lower and upper MMA tertile in serum B12 (P ? 0.001), homocysteine (P = 0.001) and neutrophil morphometric index, NeS-DW (P = 0.029). Also, serum B12 concentrations were significantly different between lower and middle MMA tertile, P = 0.005. Receiver operating characteristic analysis of NeS-DW ability to detect MMA?367 nmol/L revealed a significant area under the curve AUC = 0.761 P ? 0.001 95% CI 0.693-0.830. Optimal cut-off value was NeS-DW?3.51% with sensitivity of 74.19% and specificity of 68.87%. ConclusionIn patients at risk of cobalamin deficiency and normal MCV, classification according to MMA revealed cobalamin status differences. Neutrophil morphometric index may be an indicator of early changes in neutrophil nucleus morphology caused by impaired cobalamin status.", publisher = "Wiley-Blackwell, Hoboken", journal = "International Journal of Laboratory Hematology", title = "Methylmalonic acid and neutrophil morphometric index in laboratory diagnosis of cobalamin deficiency without macrocytosis", volume = "38", number = "3", pages = "265-272", doi = "10.1111/ijlh.12479" }
Totosković, D., Dopsaj, V.,& Martinović, J.. (2016). Methylmalonic acid and neutrophil morphometric index in laboratory diagnosis of cobalamin deficiency without macrocytosis. in International Journal of Laboratory Hematology Wiley-Blackwell, Hoboken., 38(3), 265-272. https://doi.org/10.1111/ijlh.12479
Totosković D, Dopsaj V, Martinović J. Methylmalonic acid and neutrophil morphometric index in laboratory diagnosis of cobalamin deficiency without macrocytosis. in International Journal of Laboratory Hematology. 2016;38(3):265-272. doi:10.1111/ijlh.12479 .
Totosković, Dragana, Dopsaj, Violeta, Martinović, Jelena, "Methylmalonic acid and neutrophil morphometric index in laboratory diagnosis of cobalamin deficiency without macrocytosis" in International Journal of Laboratory Hematology, 38, no. 3 (2016):265-272, https://doi.org/10.1111/ijlh.12479 . .