Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method
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2016
Authors
Milašinović, Nikola
Čalija, Bojan

Vidović, Bojana

Crevar-Sakač, Milkica

Vujić, Zorica

Knežević-Jugović, Zorica
Article (Published version)

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The aim of this study was to investigate the encapsulation of a-lipoic acid (LA) onto chitosan microbeads in order to achieve a sustained release. Microbeads of different compositions were synthetized by inverse emulsion method, and characterized by in vitro swelling and drug release studies, FT-IR, DSC and SEM analyses. The encapsulation of LA was performed by swelling of previously synthetized dry microbeads in LA solution. The encapsulation efficiency was determined in terms of LA concentration, using liquid chromatography tandem mass spectrometry (LC-MS/MS). The swelling of synthetized microbeads was well pronounced in the acidic media. FT-IR analysis confirmed the presence of LA in microbeads and its interactions with matrices, which was in agreement with the results revealed by DSC analysis. SEM analysis revealed the porous structure, micron-size and spherical shape of the synthetized beads. Encapsulation efficiency was in the range from 46.8 to 58.5% depending on the hydrogel co...mposition. The microbeads exhibited sustained release of LA under conditions mimicking gastrointestinal tract. Results of antioxidant activity of released LA showed good correlation with the concentration of the released LA. The obtained results suggested that synthetized microbeads have promising potential for oral administration with reduced dosing frequency in comparison to the immediate release formulations.
Source:
Journal of the Taiwan Institute of Chemical Engineers, 2016, 60, 106-112Publisher:
- Elsevier Science BV, Amsterdam
Funding / projects:
DOI: 10.1016/j.jtice.2015.10.037
ISSN: 1876-1070
WoS: 000371937900011
Scopus: 2-s2.0-84958745685
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PharmacyTY - JOUR AU - Milašinović, Nikola AU - Čalija, Bojan AU - Vidović, Bojana AU - Crevar-Sakač, Milkica AU - Vujić, Zorica AU - Knežević-Jugović, Zorica PY - 2016 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2607 AB - The aim of this study was to investigate the encapsulation of a-lipoic acid (LA) onto chitosan microbeads in order to achieve a sustained release. Microbeads of different compositions were synthetized by inverse emulsion method, and characterized by in vitro swelling and drug release studies, FT-IR, DSC and SEM analyses. The encapsulation of LA was performed by swelling of previously synthetized dry microbeads in LA solution. The encapsulation efficiency was determined in terms of LA concentration, using liquid chromatography tandem mass spectrometry (LC-MS/MS). The swelling of synthetized microbeads was well pronounced in the acidic media. FT-IR analysis confirmed the presence of LA in microbeads and its interactions with matrices, which was in agreement with the results revealed by DSC analysis. SEM analysis revealed the porous structure, micron-size and spherical shape of the synthetized beads. Encapsulation efficiency was in the range from 46.8 to 58.5% depending on the hydrogel composition. The microbeads exhibited sustained release of LA under conditions mimicking gastrointestinal tract. Results of antioxidant activity of released LA showed good correlation with the concentration of the released LA. The obtained results suggested that synthetized microbeads have promising potential for oral administration with reduced dosing frequency in comparison to the immediate release formulations. PB - Elsevier Science BV, Amsterdam T2 - Journal of the Taiwan Institute of Chemical Engineers T1 - Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method VL - 60 SP - 106 EP - 112 DO - 10.1016/j.jtice.2015.10.037 ER -
@article{ author = "Milašinović, Nikola and Čalija, Bojan and Vidović, Bojana and Crevar-Sakač, Milkica and Vujić, Zorica and Knežević-Jugović, Zorica", year = "2016", abstract = "The aim of this study was to investigate the encapsulation of a-lipoic acid (LA) onto chitosan microbeads in order to achieve a sustained release. Microbeads of different compositions were synthetized by inverse emulsion method, and characterized by in vitro swelling and drug release studies, FT-IR, DSC and SEM analyses. The encapsulation of LA was performed by swelling of previously synthetized dry microbeads in LA solution. The encapsulation efficiency was determined in terms of LA concentration, using liquid chromatography tandem mass spectrometry (LC-MS/MS). The swelling of synthetized microbeads was well pronounced in the acidic media. FT-IR analysis confirmed the presence of LA in microbeads and its interactions with matrices, which was in agreement with the results revealed by DSC analysis. SEM analysis revealed the porous structure, micron-size and spherical shape of the synthetized beads. Encapsulation efficiency was in the range from 46.8 to 58.5% depending on the hydrogel composition. The microbeads exhibited sustained release of LA under conditions mimicking gastrointestinal tract. Results of antioxidant activity of released LA showed good correlation with the concentration of the released LA. The obtained results suggested that synthetized microbeads have promising potential for oral administration with reduced dosing frequency in comparison to the immediate release formulations.", publisher = "Elsevier Science BV, Amsterdam", journal = "Journal of the Taiwan Institute of Chemical Engineers", title = "Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method", volume = "60", pages = "106-112", doi = "10.1016/j.jtice.2015.10.037" }
Milašinović, N., Čalija, B., Vidović, B., Crevar-Sakač, M., Vujić, Z.,& Knežević-Jugović, Z.. (2016). Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method. in Journal of the Taiwan Institute of Chemical Engineers Elsevier Science BV, Amsterdam., 60, 106-112. https://doi.org/10.1016/j.jtice.2015.10.037
Milašinović N, Čalija B, Vidović B, Crevar-Sakač M, Vujić Z, Knežević-Jugović Z. Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method. in Journal of the Taiwan Institute of Chemical Engineers. 2016;60:106-112. doi:10.1016/j.jtice.2015.10.037 .
Milašinović, Nikola, Čalija, Bojan, Vidović, Bojana, Crevar-Sakač, Milkica, Vujić, Zorica, Knežević-Jugović, Zorica, "Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method" in Journal of the Taiwan Institute of Chemical Engineers, 60 (2016):106-112, https://doi.org/10.1016/j.jtice.2015.10.037 . .