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Aging affects the responsiveness of rat peritoneal macrophages to GM-CSF and IL-4
dc.creator | Dimitrijević, Mirjana | |
dc.creator | Stanojević, Stanislava | |
dc.creator | Blagojević, Veljko | |
dc.creator | Curuvija, Ivana | |
dc.creator | Vujnović, Ivana | |
dc.creator | Petrović, Raisa | |
dc.creator | Arsenović-Ranin, Nevena | |
dc.creator | Vujić, Vesna | |
dc.creator | Leposavić, Gordana | |
dc.date.accessioned | 2019-09-02T11:52:33Z | |
dc.date.available | 2019-09-02T11:52:33Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 1389-5729 | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/2609 | |
dc.description.abstract | Macrophages undergo significant functional alterations during aging. The aim of the present study was to investigate changes of rat macrophage functions and response to M1/M2 polarization signals with age. Therefore, resident and thioglycollate-elicited peritoneal macrophages from young (3-month-old) and aged (18-19-month-old) rats were tested for phagocytic capacity and ability to secrete inflammatory mediators following in vitro stimulation with LPS and GM-CSF, and IL-4, prototypic stimulators for classically (M1) and alternatively activated (M2) macrophages, respectively. Aging increased the frequency of monocyte-derived (CCR7+ CD68+) and the most mature (CD163+ CD68+) macrophages within resident and thioglycollate-elicited peritoneal macrophages, respectively. The ability to phagocyte zymosan of none of these two cell subsets was affected by either LPS and GM-CSF or IL-4. The upregulated production of IL-1 beta, IL-6 and IL-10 and downregulated that of TGF-beta was observed in response to LPS in resident and thioglycollate-elicited macrophages from rats of both ages. GM-CSF elevated production of IL-1 beta and IL-6 in resident macrophages from aged rats and in thioglycollate-elicited macrophages from young rats. Unexpectedly, IL-4 augmented production of proinflammatory mediators, IL-1 beta and IL-6, in resident macrophages from aged rats. In both resident and thioglycollate-elicited macrophages aging decreased NO/urea ratio, whereas LPS but not GM-SCF, shifted this ratio toward NO in the macrophages from animals of both ages. Conversely, IL-4 reduced NO/urea ratio in resident and thioglycollate-elicited macrophages from young rats only. In conclusion, our study showed that aging diminished GM-CSF-triggered polarization of elicited macrophages and caused paradoxical IL-4-driven polarization of resident macrophages toward proinflammatory M1 phenotype. This age-related deregulation of macrophage inflammatory mediator secretion and phagocytosis in response to M1/M2 activators may lead to the deficient control of infectious and/or inflammatory diseases in advanced age. | en |
dc.publisher | Springer, New York | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175050/RS// | |
dc.rights | restrictedAccess | |
dc.source | Biogerontology | |
dc.title | Aging affects the responsiveness of rat peritoneal macrophages to GM-CSF and IL-4 | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Aрсеновић-Ранин, Невена; Благојевић, Вељко; Станојевић, Станислава; Цурувија, Ивана; Вујновић, Ивана; Петровић, Раиса; Димитријевић, Мирјана; Вујић, Весна; Лепосавић, Гордана; | |
dc.citation.volume | 17 | |
dc.citation.issue | 2 | |
dc.citation.spage | 359 | |
dc.citation.epage | 371 | |
dc.citation.other | 17(2): 359-371 | |
dc.citation.rank | M22 | |
dc.identifier.wos | 000373298800009 | |
dc.identifier.doi | 10.1007/s10522-015-9620-x | |
dc.identifier.pmid | 26463212 | |
dc.identifier.scopus | 2-s2.0-84944518727 | |
dc.type.version | publishedVersion |