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dc.creatorCrevar-Sakač, Milkica
dc.creatorVujić, Zorica
dc.creatorVujčić, Zoran
dc.creatorMarković, Bojan
dc.creatorVasiljević, Dragana
dc.date.accessioned2019-09-02T11:52:55Z
dc.date.available2019-09-02T11:52:55Z
dc.date.issued2016
dc.identifier.issn1233-2356
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/2623
dc.description.abstractA simple and sensitive liquid chromatography-tandem mass spectrometry method was developed for the quantification of atorvastatin, ortho-hydroxyatorvastatin, para-hydroxyatorvastatin, and atorvastatin lactone in rat plasma. Solid-phase extraction was used for preparation of samples. Rosuvastatin was chosen as an internal standard. Chromatographic separation was achieved on ZORBAX Eclipse C-18 Analytical, 4.6 x 100 mm (3.5 mu m) column with a gradient mobile phase composed of acetonitrile and 0.1% acetic acid, at a flow rate of 400 mu L min(-1). The column was kept at constant temperature (25 degrees C), and autosampler tray temperature was set at 4 degrees C. The following selected reaction monitoring (SRM) transitions were selected: (m/z, Q1 -> Q3, collision energy) atorvastatin (559.47 -> 440.03, 22 eV), atorvastatin lactone (541.36 -> 448.02, 19 eV), orthohydroxyatorvastatin (575.20 -> 440.18, 20 eV), para-hydroxyatorvastatin (575.54 -> 440.18, 20 eV), and rosuvastatin (482.25 with selected combination of two fragments 257.77, 31 eV, and 299.81, 35 eV) in positive ion mode. The method was validated over a concentration range of 0.5-20 ng mL(-1) for ortho-hydroxyatorvastatin and para-hydroxyatorvastatin and 0.1-20 ng mL(-1) for atorvastatin and atorvastatin lactone with excellent linearity (r(2) >= 0.99). This method demonstrated acceptable precision and accuracy at four quality control concentration levels. The detection limits were 0.1 and 0.13 ng mL(-1) for orthohydroxyatorvastatin and para-hydroxyatorvastatin, respectively, and 0.05 ng mL(-1) for atorvastatin and atorvastatin lactone. All analytes were found to be stable at examined conditions. Validated method was applied for determination of atorvastatin and its metabolites in plasma of experimental animals.en
dc.publisherAkademiai Kiado Rt, Budapest
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172041/RS//
dc.rightsopenAccess
dc.sourceActa Chromatographica
dc.titleLC-MS/MS Method for Quantification of Atorvastatin, o-Hydroxyatorvastatin, p-Hydroxyatorvastatin, and Atorvastatin Lactone in Rat Plasmaen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractВујић, Зорица; Цревар-Сакач, Милкица; Марковић, Бојан; Васиљевић, Драгана; Вујчић, Зоран;
dc.citation.volume28
dc.citation.issue3
dc.citation.spage281
dc.citation.epage298
dc.citation.other28(3): 281-298
dc.citation.rankM23
dc.identifier.wos000387843800002
dc.identifier.doi10.1556/1326.2016.28.3.1
dc.identifier.scopus2-s2.0-84989863847
dc.identifier.fulltexthttp://farfar.pharmacy.bg.ac.rs//bitstream/id/1301/2621.pdf
dc.identifier.rcubconv_3699
dc.type.versionpublishedVersion


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