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dc.creatorDobričić, Vladimir
dc.creatorBubić-Pajić, Nataša
dc.creatorMarković, Bojan
dc.creatorVladimirov, Sote
dc.creatorSavić, Snežana
dc.creatorVuleta, Gordana
dc.date.accessioned2019-09-02T11:52:59Z
dc.date.available2019-09-02T11:52:59Z
dc.date.issued2016
dc.identifier.issn0352-5139
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/2627
dc.description.abstractThe development and validation of a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of adapalene in pharmaceutical forms for skin application were presented in this study. The MS/MS analysis of adapalene was performed by use of three mobile phases, consisted of acetonitrile and a) 0.1 % formic acid, b) 0.1 % trifluoroacetic acid and c) 20 mM ammonium acetate. The strongest signals of parent ion and dominant product ion were obtained in negative mode by use of the mobile phase c). The validation of this method was performed according to the ICH guidelines. Small variations of selected chromatographic parameters (concentration of ammonium acetate, mobile phase composition, column temperature and flow rate) did not affect significantly the qualitative and quantitative system responses, which proved the method's robustness. The method is specific for the determination of adapalene. The linearity was proved in the concentration range of 6.7-700.0 ng mL(-1) (r = 0.9990), with limits of detection and quantification of 2.0 and 6.7 ng mL(-1), respectively. The accuracy was confirmed by calculated recoveries (98.4-101.5 %). The precision was tested at three levels: injection repeatability, analysis repeatability and intermediate precision. The calculated relative standard deviations were less than 1, 2 and 3 %, respectively.en
dc.publisherSrpsko hemijsko društvo, Beograd
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172041/RS//
dc.rightsopenAccess
dc.sourceJournal of the Serbian Chemical Society
dc.titleDevelopment and validation of an LC-MS/MS method for the determination of adapalene in pharmaceutical forms for skin applicationen
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractВладимиров, Соте; Бубић-Пајић, Наташа; Добричић, Владимир; Савић, Снежана; Марковић, Бојан; Вулета, Гордана;
dc.citation.volume81
dc.citation.issue10
dc.citation.spage1171
dc.citation.epage1181
dc.citation.other81(10): 1171-1181
dc.citation.rankM23
dc.identifier.wos000388028400007
dc.identifier.doi10.2298/JSC160215066D
dc.identifier.scopus2-s2.0-84994027727
dc.identifier.fulltexthttp://farfar.pharmacy.bg.ac.rs//bitstream/id/1305/2625.pdf
dc.identifier.rcubconv_3704
dc.type.versionpublishedVersion


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