Double positivity of the IgG isotype of both anticardiolipin and anti-beta 2gpI antibodies is associated with the highest number of vascular impairment parameters in patients with primary antiphospholipid syndrome: preliminary data

Becarević, Mirjana; Mirković, Duško; Ignjatović, Svetlana

doi:10.1007/s10067-016-3438-z

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2016

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Becarević, Mirjana

Mirković, Duško
Ignjatović, Svetlana

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Abstract

Although numerous studies investigated the association between homocysteine (Hcy), tumor necrosis factor alpha (TNF-alpha), C-reactive protein (CRP) and apolipoproteins (apo(s)) with thrombosis and/or recurrent pregnancy losses, studies that analyzed the abovementioned parameters and multiple positivity of antiphospholipid antibodies (aPL Abs) in patients with primary antiphospholipid syndrome (PAPS) are lacking. Therefore, the aim of this study was to analyze the presence of various combinations of the abovementioned parameters and their associations with clinical and/or serological features of PAPS. High-pressure liquid chromatography (HPLC) was used for determination of Hcy, while apoAI, apoB, and lipoprotein (Lp) (a) concentrations were estimated by immunonephelometry. High-sensitivity C-reactive protein (hsCRP) was measured by immunoturbidimetry. Apo (a), TNF-alpha, and aPL Abs were measured by ELISA. Various combinations of analyzed parameters (Hcy/CRP/TNF/apoAI/apoB/apo (a)/Lp (...

Source:
Clinical Rheumatology, 2016, 35, 12, 2947-2954

Publisher:

Springer London Ltd, London

Funding / projects:

Biomarkers of organ damage and dysfunction (RS-175036)

DOI: 10.1007/s10067-016-3438-z

ISSN: 0770-3198

PubMed: 27743141

WoS: 000388826200011

Scopus: 2-s2.0-84991080565

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TY  - JOUR
AU  - Becarević, Mirjana
AU  - Mirković, Duško
AU  - Ignjatović, Svetlana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2629
AB  - Although numerous studies investigated the association between homocysteine (Hcy), tumor necrosis factor alpha (TNF-alpha), C-reactive protein (CRP) and apolipoproteins (apo(s)) with thrombosis and/or recurrent pregnancy losses, studies that analyzed the abovementioned parameters and multiple positivity of antiphospholipid antibodies (aPL Abs) in patients with primary antiphospholipid syndrome (PAPS) are lacking. Therefore, the aim of this study was to analyze the presence of various combinations of the abovementioned parameters and their associations with clinical and/or serological features of PAPS. High-pressure liquid chromatography (HPLC) was used for determination of Hcy, while apoAI, apoB, and lipoprotein (Lp) (a) concentrations were estimated by immunonephelometry. High-sensitivity C-reactive protein (hsCRP) was measured by immunoturbidimetry. Apo (a), TNF-alpha, and aPL Abs were measured by ELISA. Various combinations of analyzed parameters (Hcy/CRP/TNF/apoAI/apoB/apo (a)/Lp (a)) were not associated with a single presence of either aPL Abs. Double positivity for both isotypes of anticardiolipin (aCL) Abs (IgG + IgM) was associated with the increased apoB levels. The presence of the IgG isotype of both aCL + anti-beta2 glycoprotein I (a beta 2gpI) Abs was associated with the highest number of analyzed parameters (i.e., increased levels of hsCRP, Lp (a), and apo (a)). The presence of the IgG isotype of both aCL + a beta 2gpI Abs was associated with the highest number of vascular impairment parameters in patients with PAPS, and this combination confers the highest risk for the recurrence of thrombotic episodes. This is the first report that analyzed the association between various combinations of vascular impairment parameters with multiple aPL Ab positivity. Our results provide a rationale for further investigations of therapeutic approaches for PAPS patients.
PB  - Springer London Ltd, London
T2  - Clinical Rheumatology
T1  - Double positivity of the IgG isotype of both anticardiolipin and anti-beta 2gpI antibodies is associated with the highest number of vascular impairment parameters in patients with primary antiphospholipid syndrome: preliminary data
VL  - 35
IS  - 12
SP  - 2947
EP  - 2954
DO  - 10.1007/s10067-016-3438-z
ER  -

@article{
author = "Becarević, Mirjana and Mirković, Duško and Ignjatović, Svetlana",
year = "2016",
abstract = "Although numerous studies investigated the association between homocysteine (Hcy), tumor necrosis factor alpha (TNF-alpha), C-reactive protein (CRP) and apolipoproteins (apo(s)) with thrombosis and/or recurrent pregnancy losses, studies that analyzed the abovementioned parameters and multiple positivity of antiphospholipid antibodies (aPL Abs) in patients with primary antiphospholipid syndrome (PAPS) are lacking. Therefore, the aim of this study was to analyze the presence of various combinations of the abovementioned parameters and their associations with clinical and/or serological features of PAPS. High-pressure liquid chromatography (HPLC) was used for determination of Hcy, while apoAI, apoB, and lipoprotein (Lp) (a) concentrations were estimated by immunonephelometry. High-sensitivity C-reactive protein (hsCRP) was measured by immunoturbidimetry. Apo (a), TNF-alpha, and aPL Abs were measured by ELISA. Various combinations of analyzed parameters (Hcy/CRP/TNF/apoAI/apoB/apo (a)/Lp (a)) were not associated with a single presence of either aPL Abs. Double positivity for both isotypes of anticardiolipin (aCL) Abs (IgG + IgM) was associated with the increased apoB levels. The presence of the IgG isotype of both aCL + anti-beta2 glycoprotein I (a beta 2gpI) Abs was associated with the highest number of analyzed parameters (i.e., increased levels of hsCRP, Lp (a), and apo (a)). The presence of the IgG isotype of both aCL + a beta 2gpI Abs was associated with the highest number of vascular impairment parameters in patients with PAPS, and this combination confers the highest risk for the recurrence of thrombotic episodes. This is the first report that analyzed the association between various combinations of vascular impairment parameters with multiple aPL Ab positivity. Our results provide a rationale for further investigations of therapeutic approaches for PAPS patients.",
publisher = "Springer London Ltd, London",
journal = "Clinical Rheumatology",
title = "Double positivity of the IgG isotype of both anticardiolipin and anti-beta 2gpI antibodies is associated with the highest number of vascular impairment parameters in patients with primary antiphospholipid syndrome: preliminary data",
volume = "35",
number = "12",
pages = "2947-2954",
doi = "10.1007/s10067-016-3438-z"
}

Becarević, M., Mirković, D.,& Ignjatović, S.. (2016). Double positivity of the IgG isotype of both anticardiolipin and anti-beta 2gpI antibodies is associated with the highest number of vascular impairment parameters in patients with primary antiphospholipid syndrome: preliminary data. in Clinical Rheumatology
Springer London Ltd, London., 35(12), 2947-2954.
https://doi.org/10.1007/s10067-016-3438-z

Becarević M, Mirković D, Ignjatović S. Double positivity of the IgG isotype of both anticardiolipin and anti-beta 2gpI antibodies is associated with the highest number of vascular impairment parameters in patients with primary antiphospholipid syndrome: preliminary data. in Clinical Rheumatology. 2016;35(12):2947-2954.
doi:10.1007/s10067-016-3438-z .

Becarević, Mirjana, Mirković, Duško, Ignjatović, Svetlana, "Double positivity of the IgG isotype of both anticardiolipin and anti-beta 2gpI antibodies is associated with the highest number of vascular impairment parameters in patients with primary antiphospholipid syndrome: preliminary data" in Clinical Rheumatology, 35, no. 12 (2016):2947-2954,
https://doi.org/10.1007/s10067-016-3438-z . .