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Antioxidant activity and protective role on protein glycation of synthetic aminocoumarins

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2016
2677.pdf (457.8Kb)
Authors
Aminjafari, Akram
Miroliaei, Mehran
Angelova, Violina T.
Emamzadeh, Rahman
Đukić, Mirjana
Đurić, Ana
Saso, Luciano
Article (Published version)
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Abstract
Background: Synthesized aminocoumarins are heterocyclic compounds possessing potential for the treatment of insulin-dependent diabetes mellitus with unexplored anti-glycative action. Results: In this study 4-aminocoumarin derivatives (4-ACDs) were evaluated in vitro for antiglycation (AG) activities by using the human serum albumin (HSA)/glucose system, for 8 weeks of incubation. The glycation and conformational alteration of HSA in the presence of the tested compounds were evaluated by Congo red assay, fluorescence and circular dichroism spectroscopy. The antioxidant (AO) capacity were also tested by four different assays including: DPPH (2,2'-diphenyl-1-picrylhydrazyl radical), ABTS (2,2-azinobis (3-ethylbenzothiazoline-6-sulphonate) diammonium salt), FRAP (ferric reducing antioxidant power) and beta-carotene-linoleic acid assay. The tested compounds showed AG and AO effects. The intensity of the accomplished AO potential is related to the type of the used assay. Significant alterati...ons in the secondary (monitored by CD spectropolarimetry) and tertiary structure (assessed by spectrofluorimetry) of HSA upon glycation were mitigated by the 4-ACDs, suggesting their suppressive role in the late stage (post-Amadori) of the HSA glycation. Conclusions: By the analogues, in vitro ascertained AO and AG properties of 4-ACDmay be recognized as rationale for their protective role against oxidative changes of proteins, thereby precluding diabetic complications in humans.

Source:
Electronic Journal of Biotechnology, 2016, 24, 43-48
Publisher:
  • Univ Catolica de Valparaiso, Valparaiso
Projects:
  • Iran National Science Foundation (INSF)
  • Research Council of University of Isfahan

DOI: 10.1016/j.ejbt.2016.08.004

ISSN: 0717-3458

WoS: 000389595400008

Scopus: 2-s2.0-84995701682
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URI
http://farfar.pharmacy.bg.ac.rs/handle/123456789/2679
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Pharmacy

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