Hydrogen peroxide-induced oxidative damage in peripheral blood lymphocytes from rats chronically treated with corticosterone: The protective effect of oxytocin treatment
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2016
Authors
Stanić, Dušanka
Plećaš-Solarović, Bosiljka
Petrović, Jelena

Bogavac-Stanojević, Nataša

Sopić, Miron

Kotur-Stevuljević, Jelena

Ignjatović, Svetlana

Pešić, Vesna

Article (Published version)

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Contemporary lifestyle is commonly associated with chronic stress, an environmental factor contributing to development of various psychological and somatic disorders. Increased levels of glucocorticoids, observed in the chronic stress, induce the production of reactive oxygen species leading to genotoxicity. The aim of this study was to investigate whether chronic administration of oxytocin (OXY) 10 IU/400 mu L/day, s.c., for 14 days, a hormone presumed to exert antioxidant effect, may prevent DNA damage in the comet assay of peripheral blood lymphocytes of Wistar rats treated chronically with corticosterone (CORT) 100 mg/L ad libitum, per os, for 21 days, as well as, to influence some plasma oxidative stress parameters, i.e. levels of total lipid hydroperoxide (LOOH), and malondialdehyde (MDA), and the activity of antioxidative enzyme superoxide dismutase (SOD). Even though there was no reduction in overall number of damaged cells after oxytocin treatment only, the marked increase in ...total comet score (TCS) after incubation with H2O2 in CORT group compared to controls, was absent in the CORT + OXY experimental group. Furthermore, significant decrease of highly damaged cells compared to corticosterone group was noted. Chronic oxytocin administration thus protected lymphocytes from high intensity damage that leads to cellular death. In addition, treatment with OXY along with CORT, significantly decreased concentration of LOOH in plasma, and increased SOD compared to CORT treatment only. This finding corresponds well with current reports on beneficial effects of OXY in conditions of HPA axis hyperactivity, and supports the hypothesis of OXY-mediated antioxidant action.
Source:
Chemico-Biological Interactions, 2016, 256, 134-141Publisher:
- Elsevier Ireland Ltd, Clare
Funding / projects:
- Biomarkers of organ damage and dysfunction (RS-175036)
DOI: 10.1016/j.cbi.2016.07.006
ISSN: 0009-2797
PubMed: 27402529
WoS: 000382341100015
Scopus: 2-s2.0-84978374810
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PharmacyTY - JOUR AU - Stanić, Dušanka AU - Plećaš-Solarović, Bosiljka AU - Petrović, Jelena AU - Bogavac-Stanojević, Nataša AU - Sopić, Miron AU - Kotur-Stevuljević, Jelena AU - Ignjatović, Svetlana AU - Pešić, Vesna PY - 2016 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2704 AB - Contemporary lifestyle is commonly associated with chronic stress, an environmental factor contributing to development of various psychological and somatic disorders. Increased levels of glucocorticoids, observed in the chronic stress, induce the production of reactive oxygen species leading to genotoxicity. The aim of this study was to investigate whether chronic administration of oxytocin (OXY) 10 IU/400 mu L/day, s.c., for 14 days, a hormone presumed to exert antioxidant effect, may prevent DNA damage in the comet assay of peripheral blood lymphocytes of Wistar rats treated chronically with corticosterone (CORT) 100 mg/L ad libitum, per os, for 21 days, as well as, to influence some plasma oxidative stress parameters, i.e. levels of total lipid hydroperoxide (LOOH), and malondialdehyde (MDA), and the activity of antioxidative enzyme superoxide dismutase (SOD). Even though there was no reduction in overall number of damaged cells after oxytocin treatment only, the marked increase in total comet score (TCS) after incubation with H2O2 in CORT group compared to controls, was absent in the CORT + OXY experimental group. Furthermore, significant decrease of highly damaged cells compared to corticosterone group was noted. Chronic oxytocin administration thus protected lymphocytes from high intensity damage that leads to cellular death. In addition, treatment with OXY along with CORT, significantly decreased concentration of LOOH in plasma, and increased SOD compared to CORT treatment only. This finding corresponds well with current reports on beneficial effects of OXY in conditions of HPA axis hyperactivity, and supports the hypothesis of OXY-mediated antioxidant action. PB - Elsevier Ireland Ltd, Clare T2 - Chemico-Biological Interactions T1 - Hydrogen peroxide-induced oxidative damage in peripheral blood lymphocytes from rats chronically treated with corticosterone: The protective effect of oxytocin treatment VL - 256 SP - 134 EP - 141 DO - 10.1016/j.cbi.2016.07.006 ER -
@article{ author = "Stanić, Dušanka and Plećaš-Solarović, Bosiljka and Petrović, Jelena and Bogavac-Stanojević, Nataša and Sopić, Miron and Kotur-Stevuljević, Jelena and Ignjatović, Svetlana and Pešić, Vesna", year = "2016", abstract = "Contemporary lifestyle is commonly associated with chronic stress, an environmental factor contributing to development of various psychological and somatic disorders. Increased levels of glucocorticoids, observed in the chronic stress, induce the production of reactive oxygen species leading to genotoxicity. The aim of this study was to investigate whether chronic administration of oxytocin (OXY) 10 IU/400 mu L/day, s.c., for 14 days, a hormone presumed to exert antioxidant effect, may prevent DNA damage in the comet assay of peripheral blood lymphocytes of Wistar rats treated chronically with corticosterone (CORT) 100 mg/L ad libitum, per os, for 21 days, as well as, to influence some plasma oxidative stress parameters, i.e. levels of total lipid hydroperoxide (LOOH), and malondialdehyde (MDA), and the activity of antioxidative enzyme superoxide dismutase (SOD). Even though there was no reduction in overall number of damaged cells after oxytocin treatment only, the marked increase in total comet score (TCS) after incubation with H2O2 in CORT group compared to controls, was absent in the CORT + OXY experimental group. Furthermore, significant decrease of highly damaged cells compared to corticosterone group was noted. Chronic oxytocin administration thus protected lymphocytes from high intensity damage that leads to cellular death. In addition, treatment with OXY along with CORT, significantly decreased concentration of LOOH in plasma, and increased SOD compared to CORT treatment only. This finding corresponds well with current reports on beneficial effects of OXY in conditions of HPA axis hyperactivity, and supports the hypothesis of OXY-mediated antioxidant action.", publisher = "Elsevier Ireland Ltd, Clare", journal = "Chemico-Biological Interactions", title = "Hydrogen peroxide-induced oxidative damage in peripheral blood lymphocytes from rats chronically treated with corticosterone: The protective effect of oxytocin treatment", volume = "256", pages = "134-141", doi = "10.1016/j.cbi.2016.07.006" }
Stanić, D., Plećaš-Solarović, B., Petrović, J., Bogavac-Stanojević, N., Sopić, M., Kotur-Stevuljević, J., Ignjatović, S.,& Pešić, V.. (2016). Hydrogen peroxide-induced oxidative damage in peripheral blood lymphocytes from rats chronically treated with corticosterone: The protective effect of oxytocin treatment. in Chemico-Biological Interactions Elsevier Ireland Ltd, Clare., 256, 134-141. https://doi.org/10.1016/j.cbi.2016.07.006
Stanić D, Plećaš-Solarović B, Petrović J, Bogavac-Stanojević N, Sopić M, Kotur-Stevuljević J, Ignjatović S, Pešić V. Hydrogen peroxide-induced oxidative damage in peripheral blood lymphocytes from rats chronically treated with corticosterone: The protective effect of oxytocin treatment. in Chemico-Biological Interactions. 2016;256:134-141. doi:10.1016/j.cbi.2016.07.006 .
Stanić, Dušanka, Plećaš-Solarović, Bosiljka, Petrović, Jelena, Bogavac-Stanojević, Nataša, Sopić, Miron, Kotur-Stevuljević, Jelena, Ignjatović, Svetlana, Pešić, Vesna, "Hydrogen peroxide-induced oxidative damage in peripheral blood lymphocytes from rats chronically treated with corticosterone: The protective effect of oxytocin treatment" in Chemico-Biological Interactions, 256 (2016):134-141, https://doi.org/10.1016/j.cbi.2016.07.006 . .