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dc.creatorProtić, Ana
dc.creatorRadisić, Marina
dc.creatorGolubović, Jelena
dc.creatorOtašević, Biljana
dc.creatorZečević, Mira
dc.creatorLausević, Mila
dc.date.accessioned2019-09-02T11:56:01Z
dc.date.available2019-09-02T11:56:01Z
dc.date.issued2016
dc.identifier.issn0009-5893
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/2746
dc.description.abstractUnknown degradation products of mycophenolate mofetil formed under oxidative stress conditions have been characterized via LC-MSn, using an RP-LC column (50 mm length, 2.1 mm i.d., 1.9 A mu m). The mobile phase consisted of 26 % acetonitrile and 74 % 9 mM ammonium acetate pH 5.87, the column temperature was set at 40 A degrees C, and the flow rate of the mobile phase was 400 A mu L min(-1). Four degradation products formed under the influence of 15 % hydrogen peroxide after an hour. Two of the degradation products had previously been identified as mycophenolic acid (m/z 321) and the N-oxide of mycophenolate mofetil (m/z 450). The other two unknown degradation products, denoted DP I (m/z 319) and DP II (m/z 466), were investigated in depth in order to identify their structures. The fragmentation patterns of DP I and DP II were established, analyzed, and compared to the fragmentation patterns of mycophenolate mofetil and the N-oxide of mycophenolate mofetil, which aided the elucidation of the structures of the unknown degradation products. The unknown degradation product DP I (m/z 319.2) was proposed to be an oxidized unsaturated mycophenolate aldehyde which also appeared as an ammonium adduct ion at m/z 336.3. The second unknown degradation product, DP II (m/z 466.3), was characterized as a doubly oxidized derivative of mycophenolate mofetil. A possible degradation pathway of mycophenolate mofetil under the influence of an oxidizing agent was also proposed.en
dc.publisherSpringer Heidelberg, Heidelberg
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172033/RS//
dc.rightsrestrictedAccess
dc.sourceChromatographia
dc.titleStructural Elucidation of Unknown Oxidative Degradation Products of Mycophenolate Mofetil Using LC-MSnen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractГолубовић, Јелена; Протић, Aна; Зечевић, Мира; Оташевић, Биљана; Радисић, Марина; Лаусевић, Мила;
dc.citation.volume79
dc.citation.issue13-14
dc.citation.spage919
dc.citation.epage926
dc.citation.other79(13-14): 919-926
dc.citation.rankM23
dc.identifier.wos000379157500012
dc.identifier.doi10.1007/s10337-016-3092-2
dc.identifier.scopus2-s2.0-84969793669
dc.identifier.rcubconv_3608
dc.type.versionpublishedVersion


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