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Structural Elucidation of Unknown Oxidative Degradation Products of Mycophenolate Mofetil Using LC-MSn
dc.creator | Protić, Ana | |
dc.creator | Radisić, Marina | |
dc.creator | Golubović, Jelena | |
dc.creator | Otašević, Biljana | |
dc.creator | Zečević, Mira | |
dc.creator | Lausević, Mila | |
dc.date.accessioned | 2019-09-02T11:56:01Z | |
dc.date.available | 2019-09-02T11:56:01Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 0009-5893 | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/2746 | |
dc.description.abstract | Unknown degradation products of mycophenolate mofetil formed under oxidative stress conditions have been characterized via LC-MSn, using an RP-LC column (50 mm length, 2.1 mm i.d., 1.9 A mu m). The mobile phase consisted of 26 % acetonitrile and 74 % 9 mM ammonium acetate pH 5.87, the column temperature was set at 40 A degrees C, and the flow rate of the mobile phase was 400 A mu L min(-1). Four degradation products formed under the influence of 15 % hydrogen peroxide after an hour. Two of the degradation products had previously been identified as mycophenolic acid (m/z 321) and the N-oxide of mycophenolate mofetil (m/z 450). The other two unknown degradation products, denoted DP I (m/z 319) and DP II (m/z 466), were investigated in depth in order to identify their structures. The fragmentation patterns of DP I and DP II were established, analyzed, and compared to the fragmentation patterns of mycophenolate mofetil and the N-oxide of mycophenolate mofetil, which aided the elucidation of the structures of the unknown degradation products. The unknown degradation product DP I (m/z 319.2) was proposed to be an oxidized unsaturated mycophenolate aldehyde which also appeared as an ammonium adduct ion at m/z 336.3. The second unknown degradation product, DP II (m/z 466.3), was characterized as a doubly oxidized derivative of mycophenolate mofetil. A possible degradation pathway of mycophenolate mofetil under the influence of an oxidizing agent was also proposed. | en |
dc.publisher | Springer Heidelberg, Heidelberg | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172033/RS// | |
dc.rights | restrictedAccess | |
dc.source | Chromatographia | |
dc.title | Structural Elucidation of Unknown Oxidative Degradation Products of Mycophenolate Mofetil Using LC-MSn | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Голубовић, Јелена; Протић, Aна; Зечевић, Мира; Оташевић, Биљана; Радисић, Марина; Лаусевић, Мила; | |
dc.citation.volume | 79 | |
dc.citation.issue | 13-14 | |
dc.citation.spage | 919 | |
dc.citation.epage | 926 | |
dc.citation.other | 79(13-14): 919-926 | |
dc.citation.rank | M23 | |
dc.identifier.wos | 000379157500012 | |
dc.identifier.doi | 10.1007/s10337-016-3092-2 | |
dc.identifier.scopus | 2-s2.0-84969793669 | |
dc.type.version | publishedVersion |