Downregulation of AdipoR1 is Associated with Increased Circulating Adiponectin Levels in Serbian Chronic Kidney Disease Patients

2016
Authors
Sopić, Miron
Joksić, Jelena
Spasojević-Kalimanovska, Vesna

Bogavac-Stanojević, Nataša

Simić-Ogrizović, Sanja

Kravljaca, Milica
Jelić-Ivanović, Zorana

Article (Published version)

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Background: Since the rise in plasma adiponectin levels in chronic kidney disease (CKD) patients has not yet been elucidated, we sought to investigate if patients on hemodialysis (HD) have altered expression of adiponectin receptors in peripheral blood mononuclear cells (PBMCs) compared to healthy subjects. Methods: This study included 31 patients with chronic kidney disease on HD and 33 healthy subjects (CG). Circulating adiponectin levels were measured by ELISA while AdipoR1 and AdipoR2 mRNA levels in PBMCs were determined by real-time PCR. Results: Plasma adiponectin levels were significantly higher in patients compared to control group (P=0.036). After adjustment for age, BMI and creatinine, this difference became even more significant (P=0.004). In both groups adiponectin correlated with creatinine (CG: r=-0.472, P=0.006; HD: r=-0.375, P=0.038), triglycerides (CG: r=0.490, P=0.004; HD: r=-0.488, P=0.005), insulin (CG: r=-0.386, P=0.038; HD: r=-0.506, P=0.012) and high density lipo...protein cholesterol (HDL-C) (CG: r=-0.672, P lt 0.001; HD: r=-0.584, P=0.001). Significantly lower expression of PBMCs AdipoR1 mRNA was found in patients compared to CG (P=0.034), while AdipoR2 mRNA levels were similarly expressed in PBMCs in both groups. Conclusions: Complex pathological processes in CKD cause downregulation of AdipoR1 which could ultimately influence AdipoR1 protein levels leading to a state of adiponectin resistance.
Source:
Journal of Medical Biochemistry, 2016, 35, 4, 436-442Publisher:
- Društvo medicinskih biohemičara Srbije, Beograd i Versita
Funding / projects:
DOI: 10.1515/jomb-2016-0007
ISSN: 1452-8258
PubMed: 28670196
WoS: 000386412400008
Scopus: 2-s2.0-84994810293
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PharmacyTY - JOUR AU - Sopić, Miron AU - Joksić, Jelena AU - Spasojević-Kalimanovska, Vesna AU - Bogavac-Stanojević, Nataša AU - Simić-Ogrizović, Sanja AU - Kravljaca, Milica AU - Jelić-Ivanović, Zorana PY - 2016 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2750 AB - Background: Since the rise in plasma adiponectin levels in chronic kidney disease (CKD) patients has not yet been elucidated, we sought to investigate if patients on hemodialysis (HD) have altered expression of adiponectin receptors in peripheral blood mononuclear cells (PBMCs) compared to healthy subjects. Methods: This study included 31 patients with chronic kidney disease on HD and 33 healthy subjects (CG). Circulating adiponectin levels were measured by ELISA while AdipoR1 and AdipoR2 mRNA levels in PBMCs were determined by real-time PCR. Results: Plasma adiponectin levels were significantly higher in patients compared to control group (P=0.036). After adjustment for age, BMI and creatinine, this difference became even more significant (P=0.004). In both groups adiponectin correlated with creatinine (CG: r=-0.472, P=0.006; HD: r=-0.375, P=0.038), triglycerides (CG: r=0.490, P=0.004; HD: r=-0.488, P=0.005), insulin (CG: r=-0.386, P=0.038; HD: r=-0.506, P=0.012) and high density lipoprotein cholesterol (HDL-C) (CG: r=-0.672, P lt 0.001; HD: r=-0.584, P=0.001). Significantly lower expression of PBMCs AdipoR1 mRNA was found in patients compared to CG (P=0.034), while AdipoR2 mRNA levels were similarly expressed in PBMCs in both groups. Conclusions: Complex pathological processes in CKD cause downregulation of AdipoR1 which could ultimately influence AdipoR1 protein levels leading to a state of adiponectin resistance. PB - Društvo medicinskih biohemičara Srbije, Beograd i Versita T2 - Journal of Medical Biochemistry T1 - Downregulation of AdipoR1 is Associated with Increased Circulating Adiponectin Levels in Serbian Chronic Kidney Disease Patients VL - 35 IS - 4 SP - 436 EP - 442 DO - 10.1515/jomb-2016-0007 ER -
@article{ author = "Sopić, Miron and Joksić, Jelena and Spasojević-Kalimanovska, Vesna and Bogavac-Stanojević, Nataša and Simić-Ogrizović, Sanja and Kravljaca, Milica and Jelić-Ivanović, Zorana", year = "2016", abstract = "Background: Since the rise in plasma adiponectin levels in chronic kidney disease (CKD) patients has not yet been elucidated, we sought to investigate if patients on hemodialysis (HD) have altered expression of adiponectin receptors in peripheral blood mononuclear cells (PBMCs) compared to healthy subjects. Methods: This study included 31 patients with chronic kidney disease on HD and 33 healthy subjects (CG). Circulating adiponectin levels were measured by ELISA while AdipoR1 and AdipoR2 mRNA levels in PBMCs were determined by real-time PCR. Results: Plasma adiponectin levels were significantly higher in patients compared to control group (P=0.036). After adjustment for age, BMI and creatinine, this difference became even more significant (P=0.004). In both groups adiponectin correlated with creatinine (CG: r=-0.472, P=0.006; HD: r=-0.375, P=0.038), triglycerides (CG: r=0.490, P=0.004; HD: r=-0.488, P=0.005), insulin (CG: r=-0.386, P=0.038; HD: r=-0.506, P=0.012) and high density lipoprotein cholesterol (HDL-C) (CG: r=-0.672, P lt 0.001; HD: r=-0.584, P=0.001). Significantly lower expression of PBMCs AdipoR1 mRNA was found in patients compared to CG (P=0.034), while AdipoR2 mRNA levels were similarly expressed in PBMCs in both groups. Conclusions: Complex pathological processes in CKD cause downregulation of AdipoR1 which could ultimately influence AdipoR1 protein levels leading to a state of adiponectin resistance.", publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita", journal = "Journal of Medical Biochemistry", title = "Downregulation of AdipoR1 is Associated with Increased Circulating Adiponectin Levels in Serbian Chronic Kidney Disease Patients", volume = "35", number = "4", pages = "436-442", doi = "10.1515/jomb-2016-0007" }
Sopić, M., Joksić, J., Spasojević-Kalimanovska, V., Bogavac-Stanojević, N., Simić-Ogrizović, S., Kravljaca, M.,& Jelić-Ivanović, Z.. (2016). Downregulation of AdipoR1 is Associated with Increased Circulating Adiponectin Levels in Serbian Chronic Kidney Disease Patients. in Journal of Medical Biochemistry Društvo medicinskih biohemičara Srbije, Beograd i Versita., 35(4), 436-442. https://doi.org/10.1515/jomb-2016-0007
Sopić M, Joksić J, Spasojević-Kalimanovska V, Bogavac-Stanojević N, Simić-Ogrizović S, Kravljaca M, Jelić-Ivanović Z. Downregulation of AdipoR1 is Associated with Increased Circulating Adiponectin Levels in Serbian Chronic Kidney Disease Patients. in Journal of Medical Biochemistry. 2016;35(4):436-442. doi:10.1515/jomb-2016-0007 .
Sopić, Miron, Joksić, Jelena, Spasojević-Kalimanovska, Vesna, Bogavac-Stanojević, Nataša, Simić-Ogrizović, Sanja, Kravljaca, Milica, Jelić-Ivanović, Zorana, "Downregulation of AdipoR1 is Associated with Increased Circulating Adiponectin Levels in Serbian Chronic Kidney Disease Patients" in Journal of Medical Biochemistry, 35, no. 4 (2016):436-442, https://doi.org/10.1515/jomb-2016-0007 . .