The aim of this study was to develop and validate a simple, rapid, sensitive and low-cost method for determination of rutin in tablets. The proposed spectrophotometric method is based on the formation of the Zn2+-rutin complex in methanol 70% (v/v) at pH 8.52, and detection at lambda(max)= 410 nm. The concentration range over which the response was linear was 0.3-12.2 mu g ml(-1). The limit of detection (LOD) and the limit of quantification (LOQ) were 0.21 mu g ml(-1) and 0.63 mu g ml(-1), respectively. The proposed method was successfully applied to the determination of rutin in herbal dietary supplements. The reliability of the method was checked by comparison with results obtained by the established RP-HPLC/UV method. The proposed method fulfills all aimed requirements.
Source:
Macedonian Journal of Chemistry and Chemical Engineering, 2016, 35, 1, 13-18
TY - JOUR
AU - Pavun, Leposava
AU - Jelikić-Stankov, Milena
AU - Đurđević, Predrag
AU - Ćirić, Andrija
AU - Uskoković-Marković, Snežana
PY - 2016
UR - http://farfar.pharmacy.bg.ac.rs/handle/123456789/2751
AB - The aim of this study was to develop and validate a simple, rapid, sensitive and low-cost method for determination of rutin in tablets. The proposed spectrophotometric method is based on the formation of the Zn2+-rutin complex in methanol 70% (v/v) at pH 8.52, and detection at lambda(max)= 410 nm. The concentration range over which the response was linear was 0.3-12.2 mu g ml(-1). The limit of detection (LOD) and the limit of quantification (LOQ) were 0.21 mu g ml(-1) and 0.63 mu g ml(-1), respectively. The proposed method was successfully applied to the determination of rutin in herbal dietary supplements. The reliability of the method was checked by comparison with results obtained by the established RP-HPLC/UV method. The proposed method fulfills all aimed requirements.
PB - Soc Chemists Technologists Madeconia, Skopje
T2 - Macedonian Journal of Chemistry and Chemical Engineering
T1 - Zinc complex-based determination of rutin in dietary supplements
VL - 35
IS - 1
SP - 13
EP - 18
DO - 10.20450/mjcce.2016.897
ER -
@article{
author = "Pavun, Leposava and Jelikić-Stankov, Milena and Đurđević, Predrag and Ćirić, Andrija and Uskoković-Marković, Snežana",
year = "2016",
url = "http://farfar.pharmacy.bg.ac.rs/handle/123456789/2751",
abstract = "The aim of this study was to develop and validate a simple, rapid, sensitive and low-cost method for determination of rutin in tablets. The proposed spectrophotometric method is based on the formation of the Zn2+-rutin complex in methanol 70% (v/v) at pH 8.52, and detection at lambda(max)= 410 nm. The concentration range over which the response was linear was 0.3-12.2 mu g ml(-1). The limit of detection (LOD) and the limit of quantification (LOQ) were 0.21 mu g ml(-1) and 0.63 mu g ml(-1), respectively. The proposed method was successfully applied to the determination of rutin in herbal dietary supplements. The reliability of the method was checked by comparison with results obtained by the established RP-HPLC/UV method. The proposed method fulfills all aimed requirements.",
publisher = "Soc Chemists Technologists Madeconia, Skopje",
journal = "Macedonian Journal of Chemistry and Chemical Engineering",
title = "Zinc complex-based determination of rutin in dietary supplements",
volume = "35",
number = "1",
pages = "13-18",
doi = "10.20450/mjcce.2016.897"
}
Pavun L, Jelikić-Stankov M, Đurđević P, Ćirić A, Uskoković-Marković S. Zinc complex-based determination of rutin in dietary supplements. Macedonian Journal of Chemistry and Chemical Engineering. 2016;35(1):13-18
Pavun, L., Jelikić-Stankov, M., Đurđević, P., Ćirić, A.,& Uskoković-Marković, S. (2016). Zinc complex-based determination of rutin in dietary supplements.
Macedonian Journal of Chemistry and Chemical EngineeringSoc Chemists Technologists Madeconia, Skopje., 35(1), 13-18.
https://doi.org/10.20450/mjcce.2016.897
