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dc.creatorStanković, Marija S.
dc.creatorJanjetović, Kristina
dc.creatorVelimirović, Milica
dc.creatorMilenković, Marina
dc.creatorStojković, Tihomir
dc.creatorPuskas, Nela
dc.creatorZaletel, Ivan
dc.creatorde Luka, Silvio
dc.creatorJanković, Saša
dc.creatorStefanović, Srđan
dc.creatorJapundzić-Zigon, Nina
dc.creatorPetronijević, Nataša D.
dc.creatorTrajković, Vladimir
dc.creatorTrbovich, Alexander M.
dc.date.accessioned2019-09-02T11:56:13Z
dc.date.available2019-09-02T11:56:13Z
dc.date.issued2016
dc.identifier.issn0014-4800
dc.identifier.urihttps://farfar.pharmacy.bg.ac.rs/handle/123456789/2752
dc.description.abstractAim: The aim of this study was to examine the role of IL-33/ST2 pathway in a pathogenesis of acute inflammation and its effects on tissue damage, antioxidative capacity, magnesium concentration and cytokine profile in acutely inflamed tissue. Material and methods: Male mice were randomly divided in four groups: wild-type control group (WT-C), ST2 knockout control group (KO-C), wild-type inflammatory group (WT-I), and ST2 knockout inflammatory group (KO-I). Acute inflammation was induced in WT-I and KO-I by intramuscular injection of turpentine oil, while mice in WT-C and KO-C were treated with saline. After 12 h, animals were euthanized, and blood was collected for determination of creatine kinase (CK) and aspartate transaminase (AST) activity. The treated tissue was used for histopathological analysis, determination of volume density of inflammatory infiltrate (Vdii) and necrotic fiber (Vdnf), gene expression of interleukin (IL)-33, ST2, tumor necrosis factor alpha (TNF-alpha), IL-6, IL-12p35, and transforming growth factor beta (TGF-beta), concentration of magnesium (Mg), copper (Cu), selenium (Se), manganese (Mn) and reduced glutathione (GSH), and superoxide dismutase (SOD) and glutathione peroxidase (GPx) activity. Results: Presence of inflammatory infiltration and necrosis in the treated tissue was histopathologically confirmed in WT-I and KO-I. Vdii was significantly higher in WT-I when compared to KO-I, whereas Vdnf did not significantly differ between WT-I and KO-I. CM and AST significantly increased in both inflammatory groups when compared to corresponding control groups. However, the values of CK and AST were significantly higher in WT-I than in KO-I. Mg in the treated tissue was significantly lower in WT-I in comparison to WT-C and KO-I, while there was no significant difference between KO-C and KO-I. There was no significant difference in Cu, Se, and Mn in the treated tissue between WT-C, KO-C, WT-I and KO-I. Gene expression of IL -33 in the treated tissue increased in both inflammatory groups when compared to the corresponding control groups, but it was significantly higher in KO-I than in WT-I. Gene expression of ST2 in the treated tissue was significantly higher in WT-I than in WT-C. Gene expression of TNF-alpha, IL-6, and IL-12p35 in the treated tissue was significantly higher in WT-I and KO-I than in the corresponding control groups, and IL-6 was significantly higher in KO-C than in WT-C. TGF-beta gene expression in the treated tissue was significantly higher in KO-I when compared to WT-I, while there was no difference between WT-C and KO-C. SOD activity decreased at the site of acute inflammation in both inflammatory groups, while the GPx activity increased. GSH in the treated tissue was significantly higher in KO-I than in KO-C or WT-I. Conclusion: The results of our study have indicated, to our knowledge for the first time, that IL-33/ST2 pathway plays a role in enhancing inflammation and tissue damage at the site of acute inflammation by affecting the concentration of magnesium and GSH, important for antioxidative capacity, as well as gene expression of anti-inflammatory cytokine TGF-beta.en
dc.publisherAcademic Press Inc Elsevier Science, San Diego
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41013/RS//
dc.rightsrestrictedAccess
dc.sourceExperimental and Molecular Pathology
dc.titleEffects of IL-33/ST2 pathway in acute inflammation on tissue damage, antioxidative parameters, magnesium concentration and cytokines profileen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractПускас, Нела; Трајковић, Владимир; Јапундзић-Зигон, Нина; Стојковић, Тихомир; Петронијевић, Наташа Д.; Залетел, Иван; Миленковић, Марина; Станковић, Марија С.; Јањетовић, Кристина; де Лука, Силвио; Јанковић, Саша; Велимировић, Милица; Трбовицх, Aлеxандер М.; Стефановић, Срђан;
dc.citation.volume101
dc.citation.issue1
dc.citation.spage31
dc.citation.epage37
dc.citation.other101(1): 31-37
dc.citation.rankM22
dc.identifier.wos000382416000004
dc.identifier.doi10.1016/j.yexmp.2016.05.012
dc.identifier.pmid27222019
dc.identifier.scopus2-s2.0-84975091272
dc.identifier.rcubconv_3655
dc.type.versionpublishedVersion


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